FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2804636356> ?p ?o ?g. }

• W2804636356 abstract "Abstract Homologous recombination-based gene targeting is a powerful tool for precise genome modification and has been widely used in organisms ranging from yeast to higher organisms such as Drosophila and mouse. However, gene targeting in higher plants, including the most widely used model plant Arabidopsis thaliana , remains challenging. Here we report a sequential transformation method for gene targeting in Arabidopsis . We find that parental lines expressing the bacterial endonuclease Cas9 from the egg cell- and early embryo-specific DD45 gene promoter can improve the frequency of single-guide RNA-targeted gene knock-ins and sequence replacements via homologous recombination at several endogenous sites in the Arabidopsis genome. These heritable gene targeting can be identified by regular PCR. Our approach enables routine and fine manipulation of the Arabidopsis genome." @default.
• W2804636356 created "2018-06-01" @default.
• W2804636356 creator A5007874566 @default.
• W2804636356 creator A5029464861 @default.
• W2804636356 creator A5042610567 @default.
• W2804636356 creator A5049293054 @default.
• W2804636356 creator A5066652500 @default.
• W2804636356 date "2018-05-17" @default.
• W2804636356 modified "2023-10-11" @default.
• W2804636356 title "CRISPR/Cas9-mediated gene targeting in Arabidopsis using sequential transformation" @default.
• W2804636356 cites W1570739956 @default.
• W2804636356 cites W1749960549 @default.
• W2804636356 cites W1937054661 @default.
• W2804636356 cites W1966423273 @default.
• W2804636356 cites W1971295892 @default.
• W2804636356 cites W1980201684 @default.
• W2804636356 cites W1983568123 @default.
• W2804636356 cites W1986703291 @default.
• W2804636356 cites W1993673609 @default.
• W2804636356 cites W1993834457 @default.
• W2804636356 cites W1996756493 @default.
• W2804636356 cites W1996788258 @default.
• W2804636356 cites W2015015564 @default.
• W2804636356 cites W2017303675 @default.
• W2804636356 cites W2021767455 @default.
• W2804636356 cites W2022016707 @default.
• W2804636356 cites W2037473198 @default.
• W2804636356 cites W2050480410 @default.
• W2804636356 cites W2077510216 @default.
• W2804636356 cites W2080335963 @default.
• W2804636356 cites W2089812281 @default.
• W2804636356 cites W2089832295 @default.
• W2804636356 cites W2101655911 @default.
• W2804636356 cites W2116041063 @default.
• W2804636356 cites W2118514050 @default.
• W2804636356 cites W2129086389 @default.
• W2804636356 cites W2133872029 @default.
• W2804636356 cites W2135008913 @default.
• W2804636356 cites W2136457138 @default.
• W2804636356 cites W2136656609 @default.
• W2804636356 cites W2137888977 @default.
• W2804636356 cites W2148302985 @default.
• W2804636356 cites W2149058703 @default.
• W2804636356 cites W2150798416 @default.
• W2804636356 cites W2152416923 @default.
• W2804636356 cites W2155194596 @default.
• W2804636356 cites W2157114569 @default.
• W2804636356 cites W2161802095 @default.
• W2804636356 cites W2168460045 @default.
• W2804636356 cites W2170821019 @default.
• W2804636356 cites W2172659855 @default.
• W2804636356 cites W2196121581 @default.
• W2804636356 cites W2207417194 @default.
• W2804636356 cites W2224094873 @default.
• W2804636356 cites W2263622228 @default.
• W2804636356 cites W2289813125 @default.
• W2804636356 cites W2311483216 @default.
• W2804636356 cites W2317995052 @default.
• W2804636356 cites W2342846132 @default.
• W2804636356 cites W2343876552 @default.
• W2804636356 cites W2488393959 @default.
• W2804636356 cites W2492321596 @default.
• W2804636356 cites W2519197628 @default.
• W2804636356 cites W2551575280 @default.
• W2804636356 cites W2560685378 @default.
• W2804636356 cites W25811119 @default.
• W2804636356 cites W2595015883 @default.
• W2804636356 cites W2611992562 @default.
• W2804636356 cites W2619703893 @default.
• W2804636356 cites W2949446120 @default.
• W2804636356 cites W2953171546 @default.
• W2804636356 cites W298763606 @default.
• W2804636356 cites W4231527100 @default.
• W2804636356 doi "https://doi.org/10.1038/s41467-018-04416-0" @default.
• W2804636356 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5958078" @default.
• W2804636356 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29773790" @default.
• W2804636356 hasPublicationYear "2018" @default.
• W2804636356 type Work @default.
• W2804636356 sameAs 2804636356 @default.
• W2804636356 citedByCount "164" @default.
• W2804636356 countsByYear W28046363562018 @default.
• W2804636356 countsByYear W28046363562019 @default.
• W2804636356 countsByYear W28046363562020 @default.
• W2804636356 countsByYear W28046363562021 @default.
• W2804636356 countsByYear W28046363562022 @default.
• W2804636356 countsByYear W28046363562023 @default.
• W2804636356 crossrefType "journal-article" @default.
• W2804636356 hasAuthorship W2804636356A5007874566 @default.
• W2804636356 hasAuthorship W2804636356A5029464861 @default.
• W2804636356 hasAuthorship W2804636356A5042610567 @default.
• W2804636356 hasAuthorship W2804636356A5049293054 @default.
• W2804636356 hasAuthorship W2804636356A5066652500 @default.
• W2804636356 hasBestOaLocation W28046363561 @default.
• W2804636356 hasConcept C102744134 @default.
• W2804636356 hasConcept C104317684 @default.
• W2804636356 hasConcept C111829913 @default.
• W2804636356 hasConcept C132455925 @default.
• W2804636356 hasConcept C141231307 @default.
• W2804636356 hasConcept C143065580 @default.
• W2804636356 hasConcept C144501496 @default.

• next