FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2805062009> ?p ?o ?g. }

• W2805062009 endingPage "584" @default.
• W2805062009 startingPage "575" @default.
• W2805062009 abstract "ABSTRACT Aim Wilms’ tumour 1 (WT1) is essential for normal podocyte function. Previous reports have demonstrated that the WT1 promoter is often methylated in cancers, leading to transcriptional silencing. Transforming growth factor‐β1 (TGF‐β1) is reported to down‐regulate WT1 expression in podocytes. Based on the hypothesis that epigenetic modification plays a role in this process, we examined whether TGF‐β1 affects the methylation status of WT1 regulatory regions. Methods Conditional immortalized human podocytes were treated with TGF‐β1. A human renal proximal tubular epithelial cell line (HK2), which does not express WT1, was used as a control. The degree of DNA methylation of the WT1 promoter, 5′ enhancer, intron 3 enhancer and 3′ enhancer was determined using quantitative methylation‐specific PCR, bisulfite sequencing and pyrosequencing. Results Both WT1 mRNA and protein expression were reduced by long‐term treatment with TGF‐β1. The WT1 promoter was hypomethylated, and the 5′ enhancer and intron 3 enhancer were substantially methylated in untreated podocytes. In contrast, in HK2 cells, the WT1 promoter was strongly methylated, and the 5′ enhancer and intron 3 enhancer were less methylated than in untreated podocytes. TGF‐β1 tended to increase WT1 promoter methylation, tended to decrease 5′ enhancer methylation and significantly decreased intron 3 enhancer methylation in podocytes. Methylation levels of the 3′ enhancer did not differ among untreated cells, TGF‐β1‐treated podocytes or HK2 cells. Conclusion Our data suggest that the methylation pattern of the WT1 promoter and enhancers in human podocytes are distinctive from those in HK2. Furthermore, TGF‐β1 alters the methylation levels of the WT1 promoter and enhancers in human podocytes. This modification may be relevant to the attenuation of WT1 by TGF‐β1, which could contribute to podocyte injury." @default.
• W2805062009 created "2018-06-13" @default.
• W2805062009 creator A5002633417 @default.
• W2805062009 creator A5004957967 @default.
• W2805062009 creator A5009036785 @default.
• W2805062009 creator A5022382465 @default.
• W2805062009 creator A5029280623 @default.
• W2805062009 creator A5034122874 @default.
• W2805062009 creator A5037618766 @default.
• W2805062009 date "2019-04-17" @default.
• W2805062009 modified "2023-10-02" @default.
• W2805062009 title "TGF‐β1 alters DNA methylation levels in promoter and enhancer regions of the <i>WT1</i> gene in human podocytes" @default.
• W2805062009 cites W1643500256 @default.
• W2805062009 cites W1968451804 @default.
• W2805062009 cites W1978127755 @default.
• W2805062009 cites W1978376502 @default.
• W2805062009 cites W1983228128 @default.
• W2805062009 cites W1984621232 @default.
• W2805062009 cites W1997446136 @default.
• W2805062009 cites W2004417708 @default.
• W2805062009 cites W2017731046 @default.
• W2805062009 cites W2019676554 @default.
• W2805062009 cites W2036094765 @default.
• W2805062009 cites W2038508315 @default.
• W2805062009 cites W2039508988 @default.
• W2805062009 cites W2048951606 @default.
• W2805062009 cites W2053856108 @default.
• W2805062009 cites W2054823635 @default.
• W2805062009 cites W2060446375 @default.
• W2805062009 cites W2063369009 @default.
• W2805062009 cites W2077276614 @default.
• W2805062009 cites W2108664872 @default.
• W2805062009 cites W2109273482 @default.
• W2805062009 cites W2139492356 @default.
• W2805062009 cites W2145865611 @default.
• W2805062009 cites W2149336866 @default.
• W2805062009 cites W2151367032 @default.
• W2805062009 cites W2153896122 @default.
• W2805062009 cites W2159784476 @default.
• W2805062009 cites W2219300018 @default.
• W2805062009 cites W2406712460 @default.
• W2805062009 cites W813160293 @default.
• W2805062009 doi "https://doi.org/10.1111/nep.13411" @default.
• W2805062009 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29851165" @default.
• W2805062009 hasPublicationYear "2019" @default.
• W2805062009 type Work @default.
• W2805062009 sameAs 2805062009 @default.
• W2805062009 citedByCount "5" @default.
• W2805062009 countsByYear W28050620092021 @default.
• W2805062009 countsByYear W28050620092022 @default.
• W2805062009 countsByYear W28050620092023 @default.
• W2805062009 crossrefType "journal-article" @default.
• W2805062009 hasAuthorship W2805062009A5002633417 @default.
• W2805062009 hasAuthorship W2805062009A5004957967 @default.
• W2805062009 hasAuthorship W2805062009A5009036785 @default.
• W2805062009 hasAuthorship W2805062009A5022382465 @default.
• W2805062009 hasAuthorship W2805062009A5029280623 @default.
• W2805062009 hasAuthorship W2805062009A5034122874 @default.
• W2805062009 hasAuthorship W2805062009A5037618766 @default.
• W2805062009 hasBestOaLocation W28050620091 @default.
• W2805062009 hasConcept C101762097 @default.
• W2805062009 hasConcept C104317684 @default.
• W2805062009 hasConcept C111936080 @default.
• W2805062009 hasConcept C150194340 @default.
• W2805062009 hasConcept C153911025 @default.
• W2805062009 hasConcept C190727270 @default.
• W2805062009 hasConcept C33288867 @default.
• W2805062009 hasConcept C37080517 @default.
• W2805062009 hasConcept C41091548 @default.
• W2805062009 hasConcept C502942594 @default.
• W2805062009 hasConcept C54355233 @default.
• W2805062009 hasConcept C86803240 @default.
• W2805062009 hasConcept C94671646 @default.
• W2805062009 hasConceptScore W2805062009C101762097 @default.
• W2805062009 hasConceptScore W2805062009C104317684 @default.
• W2805062009 hasConceptScore W2805062009C111936080 @default.
• W2805062009 hasConceptScore W2805062009C150194340 @default.
• W2805062009 hasConceptScore W2805062009C153911025 @default.
• W2805062009 hasConceptScore W2805062009C190727270 @default.
• W2805062009 hasConceptScore W2805062009C33288867 @default.
• W2805062009 hasConceptScore W2805062009C37080517 @default.
• W2805062009 hasConceptScore W2805062009C41091548 @default.
• W2805062009 hasConceptScore W2805062009C502942594 @default.
• W2805062009 hasConceptScore W2805062009C54355233 @default.
• W2805062009 hasConceptScore W2805062009C86803240 @default.
• W2805062009 hasConceptScore W2805062009C94671646 @default.
• W2805062009 hasFunder F4320320912 @default.
• W2805062009 hasIssue "5" @default.
• W2805062009 hasLocation W28050620091 @default.
• W2805062009 hasLocation W28050620092 @default.
• W2805062009 hasOpenAccess W2805062009 @default.
• W2805062009 hasPrimaryLocation W28050620091 @default.
• W2805062009 hasRelatedWork W2033874367 @default.
• W2805062009 hasRelatedWork W2167996825 @default.
• W2805062009 hasRelatedWork W2440715386 @default.
• W2805062009 hasRelatedWork W2980158117 @default.
• W2805062009 hasRelatedWork W3004424038 @default.
• W2805062009 hasRelatedWork W4210480614 @default.

• next