Matches in SemOpenAlex for { <https://semopenalex.org/work/W2805232019> ?p ?o ?g. }
- W2805232019 abstract "Abstract Lasso peptides are ribosomally synthesized and post-translationally modified peptides produced by bacteria. They are characterized by an unusual lariat-knot structure. Targeted genome scanning revealed a wide diversity of lasso peptides encoded in actinobacterial genomes, but cloning and heterologous expression of these clusters turned out to be problematic. To circumvent this, we developed an orthogonal expression system for heterologous production of actinobacterial lasso peptides in Streptomyces hosts based on a newly-identified regulatory circuit from Actinoalloteichus fjordicus . Six lasso peptide gene clusters, mainly originating from marine Actinobacteria , were chosen for proof-of-concept studies. By varying the Streptomyces expression hosts and a small set of culture conditions, three new lasso peptides were successfully produced and characterized by tandem MS. The newly developed expression system thus sets the stage to uncover and bioengineer the chemo-diversity of actinobacterial lasso peptides. Moreover, our data provide some considerations for future bioprospecting efforts for such peptides." @default.
- W2805232019 created "2018-06-13" @default.
- W2805232019 creator A5000812586 @default.
- W2805232019 creator A5003866201 @default.
- W2805232019 creator A5007595891 @default.
- W2805232019 creator A5035375779 @default.
- W2805232019 creator A5040085155 @default.
- W2805232019 creator A5048304089 @default.
- W2805232019 creator A5063713538 @default.
- W2805232019 creator A5064829612 @default.
- W2805232019 creator A5085419006 @default.
- W2805232019 creator A5085993833 @default.
- W2805232019 date "2018-05-29" @default.
- W2805232019 modified "2023-10-17" @default.
- W2805232019 title "An orthogonal system for heterologous expression of actinobacterial lasso peptides in Streptomyces hosts" @default.
- W2805232019 cites W125558696 @default.
- W2805232019 cites W1442130020 @default.
- W2805232019 cites W1495926848 @default.
- W2805232019 cites W1587997563 @default.
- W2805232019 cites W1827213771 @default.
- W2805232019 cites W1908709110 @default.
- W2805232019 cites W1911723573 @default.
- W2805232019 cites W1965906674 @default.
- W2805232019 cites W1971439989 @default.
- W2805232019 cites W1995525221 @default.
- W2805232019 cites W2003514067 @default.
- W2805232019 cites W2011357172 @default.
- W2805232019 cites W2011934361 @default.
- W2805232019 cites W2016146312 @default.
- W2805232019 cites W2023950913 @default.
- W2805232019 cites W2031012171 @default.
- W2805232019 cites W2041888890 @default.
- W2805232019 cites W2052591459 @default.
- W2805232019 cites W2059397761 @default.
- W2805232019 cites W2064744296 @default.
- W2805232019 cites W2064935894 @default.
- W2805232019 cites W2065392872 @default.
- W2805232019 cites W2065544983 @default.
- W2805232019 cites W2068092274 @default.
- W2805232019 cites W2078920879 @default.
- W2805232019 cites W2104854018 @default.
- W2805232019 cites W2109756237 @default.
- W2805232019 cites W2112579911 @default.
- W2805232019 cites W2125932096 @default.
- W2805232019 cites W2133580395 @default.
- W2805232019 cites W2142498662 @default.
- W2805232019 cites W2143395000 @default.
- W2805232019 cites W2148022166 @default.
- W2805232019 cites W2153723384 @default.
- W2805232019 cites W2162854463 @default.
- W2805232019 cites W2187341651 @default.
- W2805232019 cites W2190255122 @default.
- W2805232019 cites W2286914510 @default.
- W2805232019 cites W2321143980 @default.
- W2805232019 cites W2334939353 @default.
- W2805232019 cites W2335051262 @default.
- W2805232019 cites W2335399423 @default.
- W2805232019 cites W2402466092 @default.
- W2805232019 cites W2467031173 @default.
- W2805232019 cites W2491519599 @default.
- W2805232019 cites W2511965546 @default.
- W2805232019 cites W2535017472 @default.
- W2805232019 cites W2547082603 @default.
- W2805232019 cites W2547800356 @default.
- W2805232019 cites W2593189583 @default.
- W2805232019 cites W2740540641 @default.
- W2805232019 cites W2784971727 @default.
- W2805232019 cites W3100647865 @default.
- W2805232019 doi "https://doi.org/10.1038/s41598-018-26620-0" @default.
- W2805232019 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5974421" @default.
- W2805232019 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29844351" @default.
- W2805232019 hasPublicationYear "2018" @default.
- W2805232019 type Work @default.
- W2805232019 sameAs 2805232019 @default.
- W2805232019 citedByCount "25" @default.
- W2805232019 countsByYear W28052320192018 @default.
- W2805232019 countsByYear W28052320192019 @default.
- W2805232019 countsByYear W28052320192020 @default.
- W2805232019 countsByYear W28052320192021 @default.
- W2805232019 countsByYear W28052320192022 @default.
- W2805232019 crossrefType "journal-article" @default.
- W2805232019 hasAuthorship W2805232019A5000812586 @default.
- W2805232019 hasAuthorship W2805232019A5003866201 @default.
- W2805232019 hasAuthorship W2805232019A5007595891 @default.
- W2805232019 hasAuthorship W2805232019A5035375779 @default.
- W2805232019 hasAuthorship W2805232019A5040085155 @default.
- W2805232019 hasAuthorship W2805232019A5048304089 @default.
- W2805232019 hasAuthorship W2805232019A5063713538 @default.
- W2805232019 hasAuthorship W2805232019A5064829612 @default.
- W2805232019 hasAuthorship W2805232019A5085419006 @default.
- W2805232019 hasAuthorship W2805232019A5085993833 @default.
- W2805232019 hasBestOaLocation W28052320191 @default.
- W2805232019 hasConcept C104317684 @default.
- W2805232019 hasConcept C108305142 @default.
- W2805232019 hasConcept C2776180452 @default.
- W2805232019 hasConcept C2778169975 @default.
- W2805232019 hasConcept C2779920670 @default.
- W2805232019 hasConcept C2780115692 @default.
- W2805232019 hasConcept C40767141 @default.
- W2805232019 hasConcept C42062724 @default.