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• W2805274160 abstract "Naïve decellularized liver scaffold (nDLS)-based tissue engineering has been impaired by the lack of a suitable extracellular matrix (ECM) to provide “active micro-environmental” support. The present study aimed to examine whether a novel, regenerative DLS (rDLS) with an active ECM improves primary hepatocyte survival and prevents thrombosis. Methods: rDLS was obtained from a 30–55% partial hepatectomy that was maintained in vivo for 3–5 days and then perfused with detergent in vitro. Compared to nDLS generated from normal livers, rDLS possesses bioactive molecules due to the regenerative period in vivo. Primary mouse hepatocyte survival was evaluated by staining for Ki-67 and Trypan blue exclusion. Thrombosis was assessed by immunohistochemistry and ex vivo diluted whole-blood perfusion. Hemocompatibility was determined by near-infrared laser-Doppler flowmetry and heterotopic transplantation. After recellularization, rDLS contained more Ki-67-positive primary hepatocytes than nDLS. rDLS had a higher oxygen saturation and blood flow velocity and a lower expression of integrin αIIb and α4 than nDLS. Tumor necrosis factor-α, hepatocyte growth factor, interleukin-10, interleukin-6 and interleukin-1β were highly expressed throughout the rDLS, whereas expression of collagen-I, collagen-IV and thrombopoietin were lower in rDLS than in nDLS. Improved blood vessel patency was observed in rDLS both in vitro and in vivo. The results in mice were confirmed in large animals (pigs). Conclusion: rDLS is an effective DLS with an “active microenvironment” that supports primary hepatocyte survival and promotes blood vessel patency. This is the first study to demonstrate a rDLS with a blood microvessel network that promotes hepatocyte survival and resists thrombosis." @default.
• W2805274160 created "2018-06-13" @default.
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• W2805274160 date "2018-09-01" @default.
• W2805274160 modified "2023-10-14" @default.
• W2805274160 title "A novel bioscaffold with naturally-occurring extracellular matrix promotes hepatocyte survival and vessel patency in mouse models of heterologous transplantation" @default.
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• W2805274160 cites W1964539622 @default.
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• W2805274160 cites W1965707694 @default.
• W2805274160 cites W1967612782 @default.
• W2805274160 cites W1969010720 @default.
• W2805274160 cites W1973913381 @default.
• W2805274160 cites W1975163366 @default.
• W2805274160 cites W1981429240 @default.
• W2805274160 cites W1997547509 @default.
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• W2805274160 doi "https://doi.org/10.1016/j.biomaterials.2018.05.026" @default.
• W2805274160 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29885586" @default.
• W2805274160 hasPublicationYear "2018" @default.
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