FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2805419034> ?p ?o ?g. }

• W2805419034 abstract "ABSTRACT Diversity-generating retroelements (DGRs) create unparalleled levels of protein sequence variation through mutagenic retrohoming. Sequence information is transferred from an invariant template region ( TR ), through an RNA intermediate, to a protein-coding variable region. Selective infidelity at adenines during transfer is a hallmark of DGRs from disparate bacteria, archaea, and microbial viruses. We recapitulated selective infidelity in vitro for the prototypical Bordetella bacteriophage DGR. A complex of the DGR reverse transcriptase bRT and pentameric accessory variability determinant (Avd) protein along with DGR RNA were necessary and sufficient for synthesis of template-primed, covalently linked RNA-cDNA molecules, as observed in vivo . We identified RNAcDNA molecules to be branched and most plausibly linked through 2′-5′ phosphodiester bonds. Adenine-mutagenesis was intrinsic to the bRT-Avd complex, which displayed unprecedented promiscuity while reverse transcribing adenines of either DGR or non-DGR RNA templates. In contrast, bRT-Avd processivity was strictly dependent on the template, occurring only for the DGR RNA. This restriction was mainly due to a noncoding segment downstream of TR , which specifically bound Avd and created a privileged site for processive polymerization. Restriction to DGR RNA may protect the host genome from damage. These results define the early steps in a novel pathway for massive sequence diversification." @default.
• W2805419034 created "2018-06-13" @default.
• W2805419034 creator A5021828457 @default.
• W2805419034 creator A5027328699 @default.
• W2805419034 creator A5044658721 @default.
• W2805419034 creator A5048109007 @default.
• W2805419034 creator A5087052821 @default.
• W2805419034 creator A5088309742 @default.
• W2805419034 creator A5090832501 @default.
• W2805419034 date "2018-06-12" @default.
• W2805419034 modified "2023-10-03" @default.
• W2805419034 title "Template-assisted synthesis of adenine-mutagenized cDNA by a retroelement protein complex" @default.
• W2805419034 cites W155224798 @default.
• W2805419034 cites W1625830054 @default.
• W2805419034 cites W1803102843 @default.
• W2805419034 cites W1944771638 @default.
• W2805419034 cites W1954687613 @default.
• W2805419034 cites W1965141089 @default.
• W2805419034 cites W1967516224 @default.
• W2805419034 cites W1967886452 @default.
• W2805419034 cites W1972924519 @default.
• W2805419034 cites W1975902254 @default.
• W2805419034 cites W1982536924 @default.
• W2805419034 cites W1991333931 @default.
• W2805419034 cites W2003409413 @default.
• W2805419034 cites W2013637593 @default.
• W2805419034 cites W2021636529 @default.
• W2805419034 cites W2024675966 @default.
• W2805419034 cites W2027473839 @default.
• W2805419034 cites W2044529833 @default.
• W2805419034 cites W2046052383 @default.
• W2805419034 cites W2046597315 @default.
• W2805419034 cites W2056906285 @default.
• W2805419034 cites W2064928476 @default.
• W2805419034 cites W2077930115 @default.
• W2805419034 cites W2080475151 @default.
• W2805419034 cites W2083250638 @default.
• W2805419034 cites W2086166210 @default.
• W2805419034 cites W2098436054 @default.
• W2805419034 cites W2108087602 @default.
• W2805419034 cites W2108234281 @default.
• W2805419034 cites W2108580856 @default.
• W2805419034 cites W2117583060 @default.
• W2805419034 cites W2157753093 @default.
• W2805419034 cites W2162365719 @default.
• W2805419034 cites W2170551349 @default.
• W2805419034 cites W2345803327 @default.
• W2805419034 cites W2346405161 @default.
• W2805419034 cites W2508568913 @default.
• W2805419034 cites W2604389286 @default.
• W2805419034 cites W2767240189 @default.
• W2805419034 cites W2769034238 @default.
• W2805419034 cites W2769865067 @default.
• W2805419034 cites W4245457270 @default.
• W2805419034 doi "https://doi.org/10.1101/344556" @default.
• W2805419034 hasPublicationYear "2018" @default.
• W2805419034 type Work @default.
• W2805419034 sameAs 2805419034 @default.
• W2805419034 citedByCount "1" @default.
• W2805419034 countsByYear W28054190342018 @default.
• W2805419034 crossrefType "posted-content" @default.
• W2805419034 hasAuthorship W2805419034A5021828457 @default.
• W2805419034 hasAuthorship W2805419034A5027328699 @default.
• W2805419034 hasAuthorship W2805419034A5044658721 @default.
• W2805419034 hasAuthorship W2805419034A5048109007 @default.
• W2805419034 hasAuthorship W2805419034A5087052821 @default.
• W2805419034 hasAuthorship W2805419034A5088309742 @default.
• W2805419034 hasAuthorship W2805419034A5090832501 @default.
• W2805419034 hasBestOaLocation W28054190341 @default.
• W2805419034 hasConcept C104317684 @default.
• W2805419034 hasConcept C153911025 @default.
• W2805419034 hasConcept C156719811 @default.
• W2805419034 hasConcept C187882448 @default.
• W2805419034 hasConcept C194993378 @default.
• W2805419034 hasConcept C36457064 @default.
• W2805419034 hasConcept C54355233 @default.
• W2805419034 hasConcept C54458228 @default.
• W2805419034 hasConcept C552990157 @default.
• W2805419034 hasConcept C64962909 @default.
• W2805419034 hasConcept C67705224 @default.
• W2805419034 hasConcept C86803240 @default.
• W2805419034 hasConceptScore W2805419034C104317684 @default.
• W2805419034 hasConceptScore W2805419034C153911025 @default.
• W2805419034 hasConceptScore W2805419034C156719811 @default.
• W2805419034 hasConceptScore W2805419034C187882448 @default.
• W2805419034 hasConceptScore W2805419034C194993378 @default.
• W2805419034 hasConceptScore W2805419034C36457064 @default.
• W2805419034 hasConceptScore W2805419034C54355233 @default.
• W2805419034 hasConceptScore W2805419034C54458228 @default.
• W2805419034 hasConceptScore W2805419034C552990157 @default.
• W2805419034 hasConceptScore W2805419034C64962909 @default.
• W2805419034 hasConceptScore W2805419034C67705224 @default.
• W2805419034 hasConceptScore W2805419034C86803240 @default.
• W2805419034 hasLocation W28054190341 @default.
• W2805419034 hasLocation W28054190342 @default.
• W2805419034 hasLocation W28054190343 @default.
• W2805419034 hasOpenAccess W2805419034 @default.
• W2805419034 hasPrimaryLocation W28054190341 @default.
• W2805419034 hasRelatedWork W1509680800 @default.
• W2805419034 hasRelatedWork W1972866618 @default.

• next