FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2805634366> ?p ?o ?g. }

• W2805634366 abstract "Loop-mediated isothermal amplification (LAMP) is an isothermal nucleic acid amplification (iNAAT) technique known for its simplicity, sensitivity and speed. Its low-cost feature has resulted in its wide scale application, especially in low resource settings. The major disadvantage of LAMP is its heavy reliance on indirect detection methods like turbidity and non-specific dyes, which often leads to the detection of false positive results. In the present work, we have developed a direct detection approach, whereby a labelled loop probe quenched in its unbound state, fluoresces only when bound to its target (amplicon). Henceforth, referred to as Fluorescence of Loop Primer Upon Self Dequenching-LAMP (FLOS-LAMP), it allows for the sequence-specific detection of LAMP amplicons. The FLOS-LAMP concept was validated for rapid detection of the human pathogen, Varicella-zoster virus, from clinical samples. The FLOS-LAMP had a limit of detection of 500 copies of the target with a clinical sensitivity and specificity of 96.8% and 100%, respectively. The high level of specificity is a major advance and solves one of the main shortcomings of the LAMP technology, i.e. false positives. Self-quenching/de-quenching probes were further used with other LAMP primer sets and different fluorophores, thereby demonstrating its versatility and adaptability." @default.
• W2805634366 created "2018-06-13" @default.
• W2805634366 creator A5003055720 @default.
• W2805634366 creator A5017672029 @default.
• W2805634366 creator A5024647757 @default.
• W2805634366 creator A5039597079 @default.
• W2805634366 date "2018-04-03" @default.
• W2805634366 modified "2023-10-16" @default.
• W2805634366 title "Real-time Detection and Monitoring of Loop Mediated Amplification (LAMP) Reaction Using Self-quenching and De-quenching Fluorogenic Probes" @default.
• W2805634366 cites W162279249 @default.
• W2805634366 cites W1895298047 @default.
• W2805634366 cites W1896186489 @default.
• W2805634366 cites W1964759166 @default.
• W2805634366 cites W1973382808 @default.
• W2805634366 cites W1975801865 @default.
• W2805634366 cites W1978113357 @default.
• W2805634366 cites W1979974453 @default.
• W2805634366 cites W2003256070 @default.
• W2805634366 cites W2006824546 @default.
• W2805634366 cites W2009263251 @default.
• W2805634366 cites W2026710381 @default.
• W2805634366 cites W2036080216 @default.
• W2805634366 cites W2037017264 @default.
• W2805634366 cites W2041381764 @default.
• W2805634366 cites W2044136144 @default.
• W2805634366 cites W2048255163 @default.
• W2805634366 cites W2063533401 @default.
• W2805634366 cites W2066385972 @default.
• W2805634366 cites W2070721758 @default.
• W2805634366 cites W2100538295 @default.
• W2805634366 cites W2100808809 @default.
• W2805634366 cites W2105275554 @default.
• W2805634366 cites W2117240806 @default.
• W2805634366 cites W2129411233 @default.
• W2805634366 cites W2140363623 @default.
• W2805634366 cites W2141012160 @default.
• W2805634366 cites W2142539585 @default.
• W2805634366 cites W2153350094 @default.
• W2805634366 cites W2156250557 @default.
• W2805634366 cites W2156326901 @default.
• W2805634366 cites W2157463520 @default.
• W2805634366 cites W2160892791 @default.
• W2805634366 cites W2161877568 @default.
• W2805634366 cites W2164575752 @default.
• W2805634366 cites W2171820773 @default.
• W2805634366 cites W2316397625 @default.
• W2805634366 cites W2404804741 @default.
• W2805634366 cites W2482982567 @default.
• W2805634366 cites W2569168468 @default.
• W2805634366 cites W81757823 @default.
• W2805634366 doi "https://doi.org/10.1038/s41598-018-23930-1" @default.
• W2805634366 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5883045" @default.
• W2805634366 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29615801" @default.
• W2805634366 hasPublicationYear "2018" @default.
• W2805634366 type Work @default.
• W2805634366 sameAs 2805634366 @default.
• W2805634366 citedByCount "106" @default.
• W2805634366 countsByYear W28056343662018 @default.
• W2805634366 countsByYear W28056343662019 @default.
• W2805634366 countsByYear W28056343662020 @default.
• W2805634366 countsByYear W28056343662021 @default.
• W2805634366 countsByYear W28056343662022 @default.
• W2805634366 countsByYear W28056343662023 @default.
• W2805634366 crossrefType "journal-article" @default.
• W2805634366 hasAuthorship W2805634366A5003055720 @default.
• W2805634366 hasAuthorship W2805634366A5017672029 @default.
• W2805634366 hasAuthorship W2805634366A5024647757 @default.
• W2805634366 hasAuthorship W2805634366A5039597079 @default.
• W2805634366 hasBestOaLocation W28056343661 @default.
• W2805634366 hasConcept C104317684 @default.
• W2805634366 hasConcept C119128265 @default.
• W2805634366 hasConcept C120665830 @default.
• W2805634366 hasConcept C121332964 @default.
• W2805634366 hasConcept C121745418 @default.
• W2805634366 hasConcept C178790620 @default.
• W2805634366 hasConcept C185592680 @default.
• W2805634366 hasConcept C2777563447 @default.
• W2805634366 hasConcept C43617362 @default.
• W2805634366 hasConcept C49105822 @default.
• W2805634366 hasConcept C552990157 @default.
• W2805634366 hasConcept C55493867 @default.
• W2805634366 hasConcept C60635243 @default.
• W2805634366 hasConcept C8185291 @default.
• W2805634366 hasConcept C91881484 @default.
• W2805634366 hasConceptScore W2805634366C104317684 @default.
• W2805634366 hasConceptScore W2805634366C119128265 @default.
• W2805634366 hasConceptScore W2805634366C120665830 @default.
• W2805634366 hasConceptScore W2805634366C121332964 @default.
• W2805634366 hasConceptScore W2805634366C121745418 @default.
• W2805634366 hasConceptScore W2805634366C178790620 @default.
• W2805634366 hasConceptScore W2805634366C185592680 @default.
• W2805634366 hasConceptScore W2805634366C2777563447 @default.
• W2805634366 hasConceptScore W2805634366C43617362 @default.
• W2805634366 hasConceptScore W2805634366C49105822 @default.
• W2805634366 hasConceptScore W2805634366C552990157 @default.
• W2805634366 hasConceptScore W2805634366C55493867 @default.
• W2805634366 hasConceptScore W2805634366C60635243 @default.
• W2805634366 hasConceptScore W2805634366C8185291 @default.
• W2805634366 hasConceptScore W2805634366C91881484 @default.
• W2805634366 hasIssue "1" @default.

• next