Matches in SemOpenAlex for { <https://semopenalex.org/work/W2805722689> ?p ?o ?g. }
- W2805722689 endingPage "7714" @default.
- W2805722689 startingPage "7708" @default.
- W2805722689 abstract "A true-color electrochemiluminescent (ECL) imaging strategy was designed for a multi-immunoassay of proteins by coupling highly efficient polymer dots (Pdots) with dual DNA amplification. The Pdots were prepared by nanoprecipitation of poly[(9,9-dioctylfuorenyl-2,7-diyl)-alt-co-(1,4-benzo-{2,1′,3}-thiadiazole)] in the presence of poly(styrene-co-maleic anhydride) and functionalized with DNA1 that hybridized with black hole quencher-labeled DNA2 to self-quench the ECL emission. The Pdots modified Au/ITO electrode showed 100-fold stronger ECL emission than the Pdots modified ITO electrode. After the capture antibody immobilized on Au/ITO slide recognized the target protein and then reacted with biotin-labeled antibody, streptavidin and biotin-labeled oligonucleotide, respectively, a large number of DNA1 functionalized Pdots could be introduced onto the slide surface by rolling circle amplification of the oligonucleotide to trigger the enzymatically cyclic release of the Pdots from the self-quenched probes to solution in the presence of Exo III. The dual DNA amplification produced a greatly amplified ECL signal for true-color ECL imaging. Using carcinoembryonic antigen, cytokeratin-19-fragment, and neuron-specific enolase as a lung cancer-specific biomarker panel, the ECL imaging-based multi-immunoassay exhibited excellent performance with a linear range of 1 pg mL–1 to 500 ng mL–1 and limits of detection of 0.17, 0.12, and 0.22 pg mL–1, respectively. The proposed method could accurately detect these biomarkers in clinical human serum samples for lung cancer screening. The Pdots-based true-color ECL imaging approach possessed the advantages of visual analysis along with wide detection range and high sensitivity and thus has great potential in clinical application." @default.
- W2805722689 created "2018-06-13" @default.
- W2805722689 creator A5012804068 @default.
- W2805722689 creator A5017375525 @default.
- W2805722689 creator A5030432218 @default.
- W2805722689 creator A5062573259 @default.
- W2805722689 creator A5072379029 @default.
- W2805722689 date "2018-05-31" @default.
- W2805722689 modified "2023-10-11" @default.
- W2805722689 title "Electrochemiluminescent Imaging for Multi-immunoassay Sensitized by Dual DNA Amplification of Polymer Dot Signal" @default.
- W2805722689 cites W1028979225 @default.
- W2805722689 cites W1884863062 @default.
- W2805722689 cites W1934979396 @default.
- W2805722689 cites W1963428971 @default.
- W2805722689 cites W1974440523 @default.
- W2805722689 cites W1979902035 @default.
- W2805722689 cites W1982248426 @default.
- W2805722689 cites W1986233161 @default.
- W2805722689 cites W1990522741 @default.
- W2805722689 cites W1996844579 @default.
- W2805722689 cites W2008262280 @default.
- W2805722689 cites W2010354460 @default.
- W2805722689 cites W2014358738 @default.
- W2805722689 cites W2019416185 @default.
- W2805722689 cites W2035950049 @default.
- W2805722689 cites W2037988112 @default.
- W2805722689 cites W2040172043 @default.
- W2805722689 cites W2040294415 @default.
- W2805722689 cites W2053345104 @default.
- W2805722689 cites W2054308157 @default.
- W2805722689 cites W2056892594 @default.
- W2805722689 cites W2056913972 @default.
- W2805722689 cites W2057359931 @default.
- W2805722689 cites W2068664917 @default.
- W2805722689 cites W2069825538 @default.
- W2805722689 cites W2089996676 @default.
- W2805722689 cites W2091362123 @default.
- W2805722689 cites W2109338111 @default.
- W2805722689 cites W2114303511 @default.
- W2805722689 cites W2131710804 @default.
- W2805722689 cites W2236220015 @default.
- W2805722689 cites W2312510777 @default.
- W2805722689 cites W2471117741 @default.
- W2805722689 cites W2516564153 @default.
- W2805722689 cites W2522975919 @default.
- W2805722689 cites W2586259503 @default.
- W2805722689 cites W2591804865 @default.
- W2805722689 cites W2630554082 @default.
- W2805722689 cites W2733936264 @default.
- W2805722689 cites W2765710114 @default.
- W2805722689 cites W2771391965 @default.
- W2805722689 cites W4233654167 @default.
- W2805722689 cites W4236780543 @default.
- W2805722689 doi "https://doi.org/10.1021/acs.analchem.8b01610" @default.
- W2805722689 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29847924" @default.
- W2805722689 hasPublicationYear "2018" @default.
- W2805722689 type Work @default.
- W2805722689 sameAs 2805722689 @default.
- W2805722689 citedByCount "69" @default.
- W2805722689 countsByYear W28057226892018 @default.
- W2805722689 countsByYear W28057226892019 @default.
- W2805722689 countsByYear W28057226892020 @default.
- W2805722689 countsByYear W28057226892021 @default.
- W2805722689 countsByYear W28057226892022 @default.
- W2805722689 countsByYear W28057226892023 @default.
- W2805722689 crossrefType "journal-article" @default.
- W2805722689 hasAuthorship W2805722689A5012804068 @default.
- W2805722689 hasAuthorship W2805722689A5017375525 @default.
- W2805722689 hasAuthorship W2805722689A5030432218 @default.
- W2805722689 hasAuthorship W2805722689A5062573259 @default.
- W2805722689 hasAuthorship W2805722689A5072379029 @default.
- W2805722689 hasConcept C119128265 @default.
- W2805722689 hasConcept C121608353 @default.
- W2805722689 hasConcept C126322002 @default.
- W2805722689 hasConcept C129312508 @default.
- W2805722689 hasConcept C159654299 @default.
- W2805722689 hasConcept C178790620 @default.
- W2805722689 hasConcept C185592680 @default.
- W2805722689 hasConcept C203014093 @default.
- W2805722689 hasConcept C21951064 @default.
- W2805722689 hasConcept C2776190903 @default.
- W2805722689 hasConcept C2777387746 @default.
- W2805722689 hasConcept C2778204606 @default.
- W2805722689 hasConcept C2780420688 @default.
- W2805722689 hasConcept C43617362 @default.
- W2805722689 hasConcept C521977710 @default.
- W2805722689 hasConcept C552990157 @default.
- W2805722689 hasConcept C55493867 @default.
- W2805722689 hasConcept C71924100 @default.
- W2805722689 hasConcept C7452308 @default.
- W2805722689 hasConcept C82381507 @default.
- W2805722689 hasConcept C86803240 @default.
- W2805722689 hasConceptScore W2805722689C119128265 @default.
- W2805722689 hasConceptScore W2805722689C121608353 @default.
- W2805722689 hasConceptScore W2805722689C126322002 @default.
- W2805722689 hasConceptScore W2805722689C129312508 @default.
- W2805722689 hasConceptScore W2805722689C159654299 @default.
- W2805722689 hasConceptScore W2805722689C178790620 @default.