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- W2805935997 abstract "The aim of this study was to evaluate application of a crude enzymatic extract from the mixed culture of Trametes maxima (T. maxima) and Paecilomyces carneus (P. carneus) in removal of phenanthrene (PHE) from water. Since PHE belong to the polycyclic aromatic hydrocarbons (PAHs), it has been used as a model compound for bioremediation treatments. PAHs are highly stable molecules, and are resistant to conventional wastewater treatment. Production of crude extract from a mixed culture of T. maxima and P. carneus does not require special conditions yet the use of 20 g/L of glycerol as carbon source enhances laccase (Lcc) and manganese-dependent peroxidase (MnP) activities. Application of crude extracts becomes feasible because it does not require further purification. The crude enzymes entrapped in alginate beads in a fixed-bed packed column reached 94.3% ± 0.7% of PHE removal after 13 h of continuous treatment under nonsterile conditions, while the alginate beads without enzymes reached only 45.3% ± 23% of PHE removal. The difference between the removal of alginate beads with and without enzymes was the oxidative activity on the surface of the alginate beads, which was observed in the increase in the C═O group and in the X-ray spectroscopy analyses of the C 1s and O 1s regions. Beads without enzymes had less C═O groups. Polyoxyethylene sorbitan monooleate and dimethyl sulfoxide also influence enzymatic activity to carry out a level of PHE removal similar to a photocatalytic treatment." @default.
- W2805935997 created "2018-06-13" @default.
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- W2805935997 date "2018-10-01" @default.
- W2805935997 modified "2023-10-14" @default.
- W2805935997 title "Crude Enzymatic Extract from<i>Trametes maxima</i>and<i>Paecilomyces carneus</i>Mixed Culture Entrapped on Alginate for Phenanthrene Removal in Water" @default.
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- W2805935997 doi "https://doi.org/10.1089/ees.2018.0064" @default.
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