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- W2805955461 abstract "Aim: The study aimed to develop an LC–MS/MS assay to measure dermatan sulfate (DS) in human cerebrospinal fluid (CSF). Methods & results: DS was quantified by ion pairing LC–MS/MS analysis of the major disaccharides derived from chondroitinase B digestion. Artificial CSF was utilized as a surrogate for calibration curve preparation. The assay was fully validated, with a linear range of 20.0–4000 ng/ml, accuracy within ±20%, and precision of ≤20%. CSF samples from mucopolysaccharidoses (MPS) II patients showed an average of 11-fold increase in DS levels compared with controls. Conclusion: The described assay is capable of differentiating DS levels in the CSF of MPS II patients from controls and can be used to monitor disease progression and therapeutic responses." @default.
- W2805955461 created "2018-06-13" @default.
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- W2805955461 date "2018-06-01" @default.
- W2805955461 modified "2023-09-25" @default.
- W2805955461 title "A novel LC–MS/MS assay to quantify dermatan sulfate in cerebrospinal fluid as a biomarker for mucopolysaccharidosis II" @default.
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- W2805955461 doi "https://doi.org/10.4155/bio-2018-0025" @default.
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