FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2806233150> ?p ?o ?g. }

• W2806233150 endingPage "0" @default.
• W2806233150 startingPage "0" @default.
• W2806233150 abstract "Objective To investigate the role of RNA binding protein─upstream-of-N-Ras (UNR) in the development of glioma and its molecular mechanism.Methods First, bioinformatics analysis of CGGA database was performed to detect UNR expression level and prognosis of patients with glioma. Western blot and real-time PCR were used to detect UNR expression level in glioma cell lines and tissues. Next, UNR siRNAs were transfected in glioma cells, and MTS assay and scratch wound-healing assay were used to detect changes in cell proliferation and migration. Then, the candidate UNR target mRNAs were identified by analyzing the sequencing data of UNR iCLIP-seq, RNA sequencing and ribosome profiling databases of human melanoma. RNA immunoprecipitation and biotin pull-down assays were used to identify the UNR target mRNAs in glioma cells. Finally, western blot was used to detect the effect of UNR knockdown on ribosomal protein L9 (RPL9) and RPL9 protein expression level in glioma cell lines. RPL9 siRNA was transfected in A172 and T98G and the expression of vimentin in the cells was detected with western blot.Results Bioinformatics analysis showed that UNR mRNA expression level was significantly higher in high-grade glioma [Grade 2 (n=126), Grade 3 (n=51), Grade 4 (n=128), P<0.001]. UNR high expression levels were associated with poor prognosis (P=0.0177). UNR had high expression level in glioma cell lines and patient samples compared with normal cell lines and normal brain samples (P<0.01). Knockdown of UNR inhibited glioma cells migration (P<0.05), but did not inhibit glioma cells growth in three glioma cell lines. UNR binded the 3' untranslated region (UTR) of PTEN and RPL9 mRNAs. RPL9 protein was significantly highly expressed in most glioma cell lines (n=9) and knockdown of UNR resulted in a downregulation of RPL9 protein expression. Epithelial-mesenchymal transition (EMT)-related marker─vimentin was positively regulated by RPL9.Conclusions UNR could bind to the 3'UTR of PTEN and RPL9 in glioma cell lines, therefore promoting glioma cell migration and regulating the expression of RPL9. Here, we establish a link between UNR and RPL9 protein, which will provide new ideas for the further study of glioma." @default.
• W2806233150 created "2018-06-13" @default.
• W2806233150 creator A5002069152 @default.
• W2806233150 creator A5005544676 @default.
• W2806233150 creator A5019309501 @default.
• W2806233150 creator A5030135901 @default.
• W2806233150 creator A5036672607 @default.
• W2806233150 creator A5037787906 @default.
• W2806233150 creator A5050213589 @default.
• W2806233150 creator A5051687553 @default.
• W2806233150 date "2018-01-01" @default.
• W2806233150 modified "2023-10-16" @default.
• W2806233150 title "RNA-Binding Protein UNR Promotes Glioma Cell Migration and Regulates the Expression of Ribosomal Protein L9" @default.
• W2806233150 cites W1816037357 @default.
• W2806233150 cites W1975989411 @default.
• W2806233150 cites W1989079357 @default.
• W2806233150 cites W1989770493 @default.
• W2806233150 cites W2001304234 @default.
• W2806233150 cites W2008131643 @default.
• W2806233150 cites W2037111595 @default.
• W2806233150 cites W2038147585 @default.
• W2806233150 cites W2038520241 @default.
• W2806233150 cites W2072486317 @default.
• W2806233150 cites W2073483343 @default.
• W2806233150 cites W2077458983 @default.
• W2806233150 cites W2085280576 @default.
• W2806233150 cites W2099571103 @default.
• W2806233150 cites W2170673091 @default.
• W2806233150 cites W2332457454 @default.
• W2806233150 cites W2520875945 @default.
• W2806233150 cites W2543449938 @default.
• W2806233150 cites W2607129810 @default.
• W2806233150 cites W2607292654 @default.
• W2806233150 cites W2726392696 @default.
• W2806233150 cites W2739581227 @default.
• W2806233150 cites W2767903603 @default.
• W2806233150 doi "https://doi.org/10.24920/11815" @default.
• W2806233150 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30266104" @default.
• W2806233150 hasPublicationYear "2018" @default.
• W2806233150 type Work @default.
• W2806233150 sameAs 2806233150 @default.
• W2806233150 citedByCount "4" @default.
• W2806233150 countsByYear W28062331502019 @default.
• W2806233150 countsByYear W28062331502020 @default.
• W2806233150 countsByYear W28062331502022 @default.
• W2806233150 crossrefType "journal-article" @default.
• W2806233150 hasAuthorship W2806233150A5002069152 @default.
• W2806233150 hasAuthorship W2806233150A5005544676 @default.
• W2806233150 hasAuthorship W2806233150A5019309501 @default.
• W2806233150 hasAuthorship W2806233150A5030135901 @default.
• W2806233150 hasAuthorship W2806233150A5036672607 @default.
• W2806233150 hasAuthorship W2806233150A5037787906 @default.
• W2806233150 hasAuthorship W2806233150A5050213589 @default.
• W2806233150 hasAuthorship W2806233150A5051687553 @default.
• W2806233150 hasBestOaLocation W28062331501 @default.
• W2806233150 hasConcept C104317684 @default.
• W2806233150 hasConcept C105580179 @default.
• W2806233150 hasConcept C127561419 @default.
• W2806233150 hasConcept C153911025 @default.
• W2806233150 hasConcept C173396325 @default.
• W2806233150 hasConcept C22615655 @default.
• W2806233150 hasConcept C2776415932 @default.
• W2806233150 hasConcept C2778227246 @default.
• W2806233150 hasConcept C502942594 @default.
• W2806233150 hasConcept C54009773 @default.
• W2806233150 hasConcept C54355233 @default.
• W2806233150 hasConcept C62112901 @default.
• W2806233150 hasConcept C67705224 @default.
• W2806233150 hasConcept C71924100 @default.
• W2806233150 hasConcept C81885089 @default.
• W2806233150 hasConcept C86803240 @default.
• W2806233150 hasConceptScore W2806233150C104317684 @default.
• W2806233150 hasConceptScore W2806233150C105580179 @default.
• W2806233150 hasConceptScore W2806233150C127561419 @default.
• W2806233150 hasConceptScore W2806233150C153911025 @default.
• W2806233150 hasConceptScore W2806233150C173396325 @default.
• W2806233150 hasConceptScore W2806233150C22615655 @default.
• W2806233150 hasConceptScore W2806233150C2776415932 @default.
• W2806233150 hasConceptScore W2806233150C2778227246 @default.
• W2806233150 hasConceptScore W2806233150C502942594 @default.
• W2806233150 hasConceptScore W2806233150C54009773 @default.
• W2806233150 hasConceptScore W2806233150C54355233 @default.
• W2806233150 hasConceptScore W2806233150C62112901 @default.
• W2806233150 hasConceptScore W2806233150C67705224 @default.
• W2806233150 hasConceptScore W2806233150C71924100 @default.
• W2806233150 hasConceptScore W2806233150C81885089 @default.
• W2806233150 hasConceptScore W2806233150C86803240 @default.
• W2806233150 hasIssue "00" @default.
• W2806233150 hasLocation W28062331501 @default.
• W2806233150 hasLocation W28062331502 @default.
• W2806233150 hasOpenAccess W2806233150 @default.
• W2806233150 hasPrimaryLocation W28062331501 @default.
• W2806233150 hasRelatedWork W1983015622 @default.
• W2806233150 hasRelatedWork W1987600894 @default.
• W2806233150 hasRelatedWork W2494327966 @default.
• W2806233150 hasRelatedWork W2508109637 @default.
• W2806233150 hasRelatedWork W2762525387 @default.
• W2806233150 hasRelatedWork W2898598696 @default.

• next