FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2806236985> ?p ?o ?g. }

• W2806236985 endingPage "10" @default.
• W2806236985 startingPage "1" @default.
• W2806236985 abstract "Preservation of a pathogen free uterine environment is critical for maintaining healthy swine herds with high reproductive performance. Considering that uterine epithelial cells are the most numerous and thus likely point of cellular contact for pathogens in the uterus, we hypothesize that these cells may be critical for activating the immune system to clear uterine infections. Although uterine epithelial cells have not been well characterized in pigs, studies in several other species have shown that these cells express several pattern recognition receptors (PRR) and thus may act as sentinels for the uterine immune response. To characterize PRR expression in the porcine uterine epithelia, we used laser-capture microdissection to isolate epithelial cells lining the porcine uterus to quantify in vivo mRNA expression levels for select PRRs. As well, primary uterine epithelial cells (UECs) were isolated, cultured, polarized and PRR expression was quantified. Immunohistofluorescence and immunofluorescence were used to determine subcellular localization of TLR3, TLR4 and TLR9 in both uterine tissue and in polarized primary UECs. Finally, polarized primary UECs were stimulated with ligands for TLR3, TLR4, TLR9 and NOD2 to determine their functional innate immune response. Uterine epithelial cells (in vivo and in vitro) were shown to express TLR1-7, TLR9, NOD1, NOD2, NLRP3, NLRP6, NLRX1, RIG1, MDA5 and LGP2. Subcellular localization of in vivo and polarized primary UECs exhibited TLR3 and TLR9 localized to the apical cell surface whereas TLR4 was localized to the intracellular space. Polarized primary UECs stimulated with TLR3, TLR4 and TLR9 ligands showed induced secretion of IL-6, IL-13 and IL-10, respectively indicating that these receptors were functional. These results indicate that pig uterine epithelial cells are functional innate immune cells that may act as sentinels to protect against uterine infection." @default.
• W2806236985 created "2018-06-13" @default.
• W2806236985 creator A5002970194 @default.
• W2806236985 creator A5027529329 @default.
• W2806236985 creator A5027757782 @default.
• W2806236985 creator A5031042092 @default.
• W2806236985 creator A5082660964 @default.
• W2806236985 date "2018-08-01" @default.
• W2806236985 modified "2023-10-18" @default.
• W2806236985 title "Expression of pattern recognition receptors in porcine uterine epithelial cells in vivo and in culture" @default.
• W2806236985 cites W1501003333 @default.
• W2806236985 cites W1583120655 @default.
• W2806236985 cites W1628777611 @default.
• W2806236985 cites W1863323524 @default.
• W2806236985 cites W1963501822 @default.
• W2806236985 cites W1964267254 @default.
• W2806236985 cites W1964584587 @default.
• W2806236985 cites W1973399759 @default.
• W2806236985 cites W1982029015 @default.
• W2806236985 cites W1983250899 @default.
• W2806236985 cites W1992499848 @default.
• W2806236985 cites W2000023984 @default.
• W2806236985 cites W2009169674 @default.
• W2806236985 cites W2013502178 @default.
• W2806236985 cites W2018792121 @default.
• W2806236985 cites W2027123878 @default.
• W2806236985 cites W2042962853 @default.
• W2806236985 cites W2044446807 @default.
• W2806236985 cites W2049088903 @default.
• W2806236985 cites W2055177612 @default.
• W2806236985 cites W2067633650 @default.
• W2806236985 cites W2070149147 @default.
• W2806236985 cites W2092294231 @default.
• W2806236985 cites W2095401221 @default.
• W2806236985 cites W2109491705 @default.
• W2806236985 cites W2113646062 @default.
• W2806236985 cites W2124996456 @default.
• W2806236985 cites W2126659405 @default.
• W2806236985 cites W2134219545 @default.
• W2806236985 cites W2143516276 @default.
• W2806236985 cites W2151730419 @default.
• W2806236985 cites W2153842328 @default.
• W2806236985 cites W2160085982 @default.
• W2806236985 cites W2164278911 @default.
• W2806236985 cites W2312601304 @default.
• W2806236985 cites W2743010921 @default.
• W2806236985 cites W2783029706 @default.
• W2806236985 cites W580600053 @default.
• W2806236985 doi "https://doi.org/10.1016/j.vetimm.2018.06.006" @default.
• W2806236985 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30078581" @default.
• W2806236985 hasPublicationYear "2018" @default.
• W2806236985 type Work @default.
• W2806236985 sameAs 2806236985 @default.
• W2806236985 citedByCount "11" @default.
• W2806236985 countsByYear W28062369852019 @default.
• W2806236985 countsByYear W28062369852020 @default.
• W2806236985 countsByYear W28062369852021 @default.
• W2806236985 countsByYear W28062369852022 @default.
• W2806236985 countsByYear W28062369852023 @default.
• W2806236985 crossrefType "journal-article" @default.
• W2806236985 hasAuthorship W2806236985A5002970194 @default.
• W2806236985 hasAuthorship W2806236985A5027529329 @default.
• W2806236985 hasAuthorship W2806236985A5027757782 @default.
• W2806236985 hasAuthorship W2806236985A5031042092 @default.
• W2806236985 hasAuthorship W2806236985A5082660964 @default.
• W2806236985 hasConcept C104317684 @default.
• W2806236985 hasConcept C111289621 @default.
• W2806236985 hasConcept C136449434 @default.
• W2806236985 hasConcept C150194340 @default.
• W2806236985 hasConcept C150903083 @default.
• W2806236985 hasConcept C170493617 @default.
• W2806236985 hasConcept C190727270 @default.
• W2806236985 hasConcept C203014093 @default.
• W2806236985 hasConcept C207001950 @default.
• W2806236985 hasConcept C2776070231 @default.
• W2806236985 hasConcept C2776709828 @default.
• W2806236985 hasConcept C2777291061 @default.
• W2806236985 hasConcept C2778420691 @default.
• W2806236985 hasConcept C2779138994 @default.
• W2806236985 hasConcept C2781236682 @default.
• W2806236985 hasConcept C55493867 @default.
• W2806236985 hasConcept C62478195 @default.
• W2806236985 hasConcept C7573103 @default.
• W2806236985 hasConcept C86803240 @default.
• W2806236985 hasConcept C8891405 @default.
• W2806236985 hasConcept C95444343 @default.
• W2806236985 hasConceptScore W2806236985C104317684 @default.
• W2806236985 hasConceptScore W2806236985C111289621 @default.
• W2806236985 hasConceptScore W2806236985C136449434 @default.
• W2806236985 hasConceptScore W2806236985C150194340 @default.
• W2806236985 hasConceptScore W2806236985C150903083 @default.
• W2806236985 hasConceptScore W2806236985C170493617 @default.
• W2806236985 hasConceptScore W2806236985C190727270 @default.
• W2806236985 hasConceptScore W2806236985C203014093 @default.
• W2806236985 hasConceptScore W2806236985C207001950 @default.
• W2806236985 hasConceptScore W2806236985C2776070231 @default.
• W2806236985 hasConceptScore W2806236985C2776709828 @default.
• W2806236985 hasConceptScore W2806236985C2777291061 @default.

• next