FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2806242092> ?p ?o ?g. }

• W2806242092 endingPage "623" @default.
• W2806242092 startingPage "613" @default.
• W2806242092 abstract "Regulatory guidelines require the sponsors to provide assurance of clonality of the production cell line, and when such evidence is not available, additional studies are typically required to further ensure consistent long-term manufacturing of the product. One potential approach to provide such assurance of clonal derivation of a production cell line is to characterize subclones generated from the original cell line and assess their phenotypic and genotypic similarity with the hypothesis that cell lines derived from a clonal bank will share performance, productivity and product quality characteristics. In this study, a production cell line that was cloned by a validated FACS approach coupled with day 0 imaging for verification of single-cell deposition was subcloned using validated FACS and imaging methods. A total of 46 subclones were analyzed for growth, productivity, product quality, copy number, and integration site analysis. Significant diversity in cell growth, protein productivity, product quality attributes, and copy number was observed between the subclones, despite stability of the parent clone over time. The diversity in protein productivity and quality of the subclones were reproduced across time and production scales, suggesting that the resulting population post sub-cloning originating from a single cell is stable but with unique properties. Overall, this work demonstrates that the characteristics of isolated subclones are not predictive of a clonally derived parental clone. Consequently, the analysis of subclones may not be an effective approach to demonstrate clonal origin of a cell bank. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:613–623, 2018" @default.
• W2806242092 created "2018-06-13" @default.
• W2806242092 creator A5028545931 @default.
• W2806242092 creator A5033648075 @default.
• W2806242092 creator A5037776223 @default.
• W2806242092 creator A5043666824 @default.
• W2806242092 creator A5044692469 @default.
• W2806242092 creator A5052246733 @default.
• W2806242092 creator A5061831769 @default.
• W2806242092 creator A5074769495 @default.
• W2806242092 creator A5081552952 @default.
• W2806242092 creator A5088327133 @default.
• W2806242092 date "2018-05-01" @default.
• W2806242092 modified "2023-10-16" @default.
• W2806242092 title "Characterization of phenotypic and genotypic diversity in subclones derived from a clonal cell line" @default.
• W2806242092 cites W1758061342 @default.
• W2806242092 cites W1909944287 @default.
• W2806242092 cites W1924851260 @default.
• W2806242092 cites W1971085275 @default.
• W2806242092 cites W1990149904 @default.
• W2806242092 cites W1996759600 @default.
• W2806242092 cites W2027716659 @default.
• W2806242092 cites W2031200858 @default.
• W2806242092 cites W2053284223 @default.
• W2806242092 cites W2066499437 @default.
• W2806242092 cites W2085754069 @default.
• W2806242092 cites W2087783847 @default.
• W2806242092 cites W2093132278 @default.
• W2806242092 cites W2106342149 @default.
• W2806242092 cites W2115124797 @default.
• W2806242092 cites W2137334007 @default.
• W2806242092 cites W2155218352 @default.
• W2806242092 cites W2171020364 @default.
• W2806242092 cites W2204063000 @default.
• W2806242092 cites W2254007717 @default.
• W2806242092 cites W2340837533 @default.
• W2806242092 cites W2597047713 @default.
• W2806242092 cites W2604975625 @default.
• W2806242092 cites W2607237411 @default.
• W2806242092 cites W2616082873 @default.
• W2806242092 cites W2619770944 @default.
• W2806242092 cites W2751799374 @default.
• W2806242092 cites W2754820805 @default.
• W2806242092 cites W2774505065 @default.
• W2806242092 cites W2783705227 @default.
• W2806242092 doi "https://doi.org/10.1002/btpr.2666" @default.
• W2806242092 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6099272" @default.
• W2806242092 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29882350" @default.
• W2806242092 hasPublicationYear "2018" @default.
• W2806242092 type Work @default.
• W2806242092 sameAs 2806242092 @default.
• W2806242092 citedByCount "29" @default.
• W2806242092 countsByYear W28062420922019 @default.
• W2806242092 countsByYear W28062420922020 @default.
• W2806242092 countsByYear W28062420922021 @default.
• W2806242092 countsByYear W28062420922022 @default.
• W2806242092 countsByYear W28062420922023 @default.
• W2806242092 crossrefType "journal-article" @default.
• W2806242092 hasAuthorship W2806242092A5028545931 @default.
• W2806242092 hasAuthorship W2806242092A5033648075 @default.
• W2806242092 hasAuthorship W2806242092A5037776223 @default.
• W2806242092 hasAuthorship W2806242092A5043666824 @default.
• W2806242092 hasAuthorship W2806242092A5044692469 @default.
• W2806242092 hasAuthorship W2806242092A5052246733 @default.
• W2806242092 hasAuthorship W2806242092A5061831769 @default.
• W2806242092 hasAuthorship W2806242092A5074769495 @default.
• W2806242092 hasAuthorship W2806242092A5081552952 @default.
• W2806242092 hasAuthorship W2806242092A5088327133 @default.
• W2806242092 hasBestOaLocation W28062420921 @default.
• W2806242092 hasConcept C104317684 @default.
• W2806242092 hasConcept C121050878 @default.
• W2806242092 hasConcept C127716648 @default.
• W2806242092 hasConcept C135763542 @default.
• W2806242092 hasConcept C139719470 @default.
• W2806242092 hasConcept C144024400 @default.
• W2806242092 hasConcept C1491633281 @default.
• W2806242092 hasConcept C149923435 @default.
• W2806242092 hasConcept C162324750 @default.
• W2806242092 hasConcept C199360897 @default.
• W2806242092 hasConcept C204983608 @default.
• W2806242092 hasConcept C2908647359 @default.
• W2806242092 hasConcept C41008148 @default.
• W2806242092 hasConcept C54355233 @default.
• W2806242092 hasConcept C62112901 @default.
• W2806242092 hasConcept C70721500 @default.
• W2806242092 hasConcept C81089528 @default.
• W2806242092 hasConcept C81885089 @default.
• W2806242092 hasConcept C86803240 @default.
• W2806242092 hasConceptScore W2806242092C104317684 @default.
• W2806242092 hasConceptScore W2806242092C121050878 @default.
• W2806242092 hasConceptScore W2806242092C127716648 @default.
• W2806242092 hasConceptScore W2806242092C135763542 @default.
• W2806242092 hasConceptScore W2806242092C139719470 @default.
• W2806242092 hasConceptScore W2806242092C144024400 @default.
• W2806242092 hasConceptScore W2806242092C1491633281 @default.
• W2806242092 hasConceptScore W2806242092C149923435 @default.
• W2806242092 hasConceptScore W2806242092C162324750 @default.
• W2806242092 hasConceptScore W2806242092C199360897 @default.

• next