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• W2806266160 abstract "High-depth next generation sequencing data provide valuable insights into the number and distribution of RNA editing events. Here, we report the RNA editing events at cellular level of human primary monocyte using high-depth whole genomic and transcriptomic sequencing data. We identified over a ten thousand putative RNA editing sites and 69% of the sites were A-to-I editing sites. The sites enriched in repetitive sequences and intronic regions. High-depth sequencing datasets revealed that 90% of the canonical sites were edited at lower frequencies (<0.7). Single and multiple human monocytes and brain tissues samples were analyzed through genome sequence independent approach. The later approach was observed to identify more editing sites. Monocytes was observed to contain more C-to-U editing sites compared to brain tissues. Our results establish comparable pipeline that can address current limitations as well as demonstrate the potential for highly sensitive detection of RNA editing events in single cell type." @default.
• W2806266160 created "2018-06-13" @default.
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• W2806266160 date "2019-07-01" @default.
• W2806266160 modified "2023-10-17" @default.
• W2806266160 title "Transcriptogenomics identification and characterization of RNA editing sites in human primary monocytes using high-depth next generation sequencing data" @default.
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• W2806266160 doi "https://doi.org/10.1016/j.ygeno.2018.05.019" @default.
• W2806266160 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29885984" @default.
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