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- W2806281520 abstract "PURPOSE: Notch and Mechanistic Target of Rapamycin (mTOR) are important for myogenesis but their interaction is not well studied. The purpose of this project was to determine if Notch affects mTOR to correlate myotube fusion of C2C12s using a Notch inhibitor, Gamma Secretase Inhibitor (GSI) and mTOR inhibitor, Rapamycin. METHODS: C2C12s were seeded, proliferated to 90-100% confluence and then differentiated for four days. At the onset of differentiation, C2C12 cells were treated every 12 hours with one of the following conditions: 4 μmoL of GSI, 100 nmoL of Rapamycin, both, or control. At four days post-differentiation, the C2C12 cells were either fixed for immunofluorence and myotube fusion and area were determined, or were collected for western blot analysis for measurement of mTOR signaling protein expression. RESULTS: GSI treatment increased fusion index compared to the other three treatments (fusing nuclei/total nuclei) (p < 0.0001). Rapamycin subsided fusion index relative to control (p < 0.0001). GSI and Rapamycin also decreased fusion index compared to control (p < 0.0001). GSI-treated myotubes displayed elevated p-mTORSer2448 compared to control (p < 0.05). Rapamycin and GSI+Rapamycin treated myotubes displayed reduced p-mTORSer2447 expression compared to control and GSI (p < 0.05). CONCLUSIONS: Our data suggests that Notch is inhibited and mTOR activated for myotube fusion to occur." @default.
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- W2806281520 date "2018-05-01" @default.
- W2806281520 modified "2023-09-26" @default.
- W2806281520 title "Do Notch and mTOR Correlate for Myotube Formation In C2C12s?" @default.
- W2806281520 doi "https://doi.org/10.1249/01.mss.0000536907.24332.e7" @default.
- W2806281520 hasPublicationYear "2018" @default.
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