FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2806484916> ?p ?o ?g. }

• W2806484916 abstract "ABSTRACT A pathway for CFTR degradation is initiated by Hsp27 which cooperates with Ubc9 and binds to the common F508del mutant to modify it with SUMO-2/3. These SUMO paralogs form poly-chains, which are recognized by the ubiquitin ligase, RNF4, for proteosomal degradation. Here, protein array analysis identified the SUMO E3, PIAS4, which increased WT and F508del CFTR biogenesis in CFBE airway cells. PIAS4 increased immature CFTR three-fold and doubled expression of mature CFTR, detected by biochemical and functional assays. In cycloheximide chase assays, PIAS4 slowed immature F508del degradation 3-fold and stabilized mature WT CFTR at the PM. PIAS4 knockdown reduced WT and F508del CFTR expression by 40-50%, suggesting a physiological role in CFTR biogenesis. PIAS4 modified F508del CFTR with SUMO-1 in vivo and reduced its conjugation to SUMO-2/3. These SUMO paralog specific effects of PIAS4 were reproduced in vitro using purified F508del NBD1 and SUMOylation reaction components. PIAS4 reduced endogenous ubiquitin conjugation to F508del CFTR by ~50%, and blocked the impact of RNF4 on mutant CFTR disposal. These findings indicate that different SUMO paralogs determine the fates of WT and mutant CFTRs, and they suggest that a paralog switch during biogenesis can direct these proteins to different outcomes: biogenesis vs. degradation." @default.
• W2806484916 created "2018-06-13" @default.
• W2806484916 creator A5026190252 @default.
• W2806484916 creator A5037544930 @default.
• W2806484916 creator A5046421753 @default.
• W2806484916 creator A5057490932 @default.
• W2806484916 creator A5064105582 @default.
• W2806484916 creator A5073715173 @default.
• W2806484916 creator A5076006747 @default.
• W2806484916 date "2018-06-07" @default.
• W2806484916 modified "2023-09-27" @default.
• W2806484916 title "Different SUMO Paralogs Determine the Fate of WT and Mutant CFTRs: Biogenesis vs. Degradation" @default.
• W2806484916 cites W1233554811 @default.
• W2806484916 cites W1520171546 @default.
• W2806484916 cites W1606812696 @default.
• W2806484916 cites W188963926 @default.
• W2806484916 cites W1944380643 @default.
• W2806484916 cites W19539656 @default.
• W2806484916 cites W1970886429 @default.
• W2806484916 cites W1993860856 @default.
• W2806484916 cites W1995692151 @default.
• W2806484916 cites W1996779865 @default.
• W2806484916 cites W1999746307 @default.
• W2806484916 cites W2005971954 @default.
• W2806484916 cites W2006905408 @default.
• W2806484916 cites W2008559063 @default.
• W2806484916 cites W2015357935 @default.
• W2806484916 cites W2016776278 @default.
• W2806484916 cites W2021044341 @default.
• W2806484916 cites W2021992655 @default.
• W2806484916 cites W2040690917 @default.
• W2806484916 cites W2043927273 @default.
• W2806484916 cites W2049696186 @default.
• W2806484916 cites W2053929225 @default.
• W2806484916 cites W2054320133 @default.
• W2806484916 cites W2054879089 @default.
• W2806484916 cites W2057846579 @default.
• W2806484916 cites W2059699662 @default.
• W2806484916 cites W2061093635 @default.
• W2806484916 cites W2065444910 @default.
• W2806484916 cites W2067262379 @default.
• W2806484916 cites W2069156386 @default.
• W2806484916 cites W2071407356 @default.
• W2806484916 cites W2080434522 @default.
• W2806484916 cites W2089453574 @default.
• W2806484916 cites W2090289021 @default.
• W2806484916 cites W2100200896 @default.
• W2806484916 cites W2102886871 @default.
• W2806484916 cites W2106064900 @default.
• W2806484916 cites W2107156397 @default.
• W2806484916 cites W2113445876 @default.
• W2806484916 cites W2116328441 @default.
• W2806484916 cites W2117811962 @default.
• W2806484916 cites W2130619361 @default.
• W2806484916 cites W2134025840 @default.
• W2806484916 cites W2137704498 @default.
• W2806484916 cites W2139398847 @default.
• W2806484916 cites W2146335990 @default.
• W2806484916 cites W2148602450 @default.
• W2806484916 cites W2151837778 @default.
• W2806484916 cites W2152355434 @default.
• W2806484916 cites W2158824937 @default.
• W2806484916 cites W2159994070 @default.
• W2806484916 cites W2165019570 @default.
• W2806484916 cites W2166608358 @default.
• W2806484916 cites W2167536088 @default.
• W2806484916 cites W2170032136 @default.
• W2806484916 cites W2264953651 @default.
• W2806484916 cites W2402259557 @default.
• W2806484916 cites W2462325035 @default.
• W2806484916 cites W2490844245 @default.
• W2806484916 cites W2558997250 @default.
• W2806484916 cites W2588387286 @default.
• W2806484916 cites W2597003300 @default.
• W2806484916 cites W2754125972 @default.
• W2806484916 cites W4236923426 @default.
• W2806484916 cites W60430684 @default.
• W2806484916 doi "https://doi.org/10.1101/340851" @default.
• W2806484916 hasPublicationYear "2018" @default.
• W2806484916 type Work @default.
• W2806484916 sameAs 2806484916 @default.
• W2806484916 citedByCount "0" @default.
• W2806484916 crossrefType "posted-content" @default.
• W2806484916 hasAuthorship W2806484916A5026190252 @default.
• W2806484916 hasAuthorship W2806484916A5037544930 @default.
• W2806484916 hasAuthorship W2806484916A5046421753 @default.
• W2806484916 hasAuthorship W2806484916A5057490932 @default.
• W2806484916 hasAuthorship W2806484916A5064105582 @default.
• W2806484916 hasAuthorship W2806484916A5073715173 @default.
• W2806484916 hasAuthorship W2806484916A5076006747 @default.
• W2806484916 hasBestOaLocation W28064849161 @default.
• W2806484916 hasConcept C104317684 @default.
• W2806484916 hasConcept C131934819 @default.
• W2806484916 hasConcept C134459356 @default.
• W2806484916 hasConcept C138599941 @default.
• W2806484916 hasConcept C143065580 @default.
• W2806484916 hasConcept C144519050 @default.
• W2806484916 hasConcept C153911025 @default.
• W2806484916 hasConcept C171350616 @default.
• W2806484916 hasConcept C173396325 @default.

• next