Matches in SemOpenAlex for { <https://semopenalex.org/work/W2806760957> ?p ?o ?g. }
- W2806760957 endingPage "1459" @default.
- W2806760957 startingPage "1449" @default.
- W2806760957 abstract "LncRNAs and EGFRvIII sequestered in TEPs enable blood-based NSCLC diagnosis Chang-Liang Luo,1,* Zhi-Gao Xu,2,* Hao Chen,2 Jia Ji,1 Yu-Hui Wang,1 Wei Hu,1 Kun Wang,3 Wu-Wen Zhang,1 Chun-Hui Yuan,4 Fu-Bing Wang1 1Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan, China; 2Department of Pathology, Zhongnan Hospital of Wuhan University, Wuhan, China; 3Department of Laboratory Medicine, Hubei Cancer Hospital, Wuhan, China; 4Department of Laboratory Medicine, Wuhan Children’s Hospital, Huazhong University of Science and Technology, Wuhan, China *These authors contributed equally to this work Background: Tissue biopsy-based cancer diagnosis has limitations because of the fact that tumor tissues are in constant evolution and extremely heterogeneous. The current study was aimed to examine whether tumor-educated blood platelets (TEPs) might be a potential all-in-one source for blood-based cancer diagnostics to overcome the limitations of conventional cancer biopsy.Methods: In the present study, we evaluated the expression pattern of MAGI2 antisense RNA 3 (MAGI2-AS3) and ZNFX1 antisense RNA 1 (ZFAS1) in both plasma and platelets of 101 non-small-cell lung cancer (NSCLC) patients. Receiver operating characteristic (ROC) curve was generated to evaluate their diagnostic potential. In addition, epidermal growth factor receptor (EGFR) mutations were detected in DNA and RNA samples of platelets for companion diagnostics.Results: Our results showed that the levels of MAGI2-AS3 and ZFAS1 in both plasma and platelets of NSCLC patients were significantly downregulated than those in healthy controls. A positive correlation of long noncoding RNA expression was observed between platelets and plasma (r=0.738 for MAGI2-AS3, r=0.751 for ZFAS1, respectively). By ROC analysis, we found that molecular interrogation of MAGI2-AS3 and ZFAS1 in TEPs and plasma can offer valuable diagnostic performance for NSCLC patients (area under the ROC curve [AUC]MAGI2-AS3=0.853/0.892, and AUCZFAS1=0.780/0.744 for diagnosing adenocarcinoma and squamous cell carcinoma cases from controls, respectively). Clinicopathologic characteristic analysis further revealed that MAGI2-AS3 level significantly correlated with tumor–node–metastasis (TNM) stage (p=0.001 in TEPs, p=0.003 in plasma), lymph-node metastasis (p=0.016 in TEPs, p=0.023 in plasma), and distant metastasis (p=0.045 in TEPs, p=0.045 in plasma), while ZFAS1 level was only correlated with TNM stage (p=0.005 in TEPs, p=0.044 in plasma). Furthermore, EGFRvIII RNA existed in both TEPs and plasma, but EGFR intracellular mutations cannot be detected in DNA of TEPs isolated from NSCLC.Conclusion: Our data suggested that TEP is a promising source for NSCLC diagnosis and companion diagnostics. Keywords: tumor-educated platelets, lncRNA-MAGI2-AS3, lncRNA-ZFAS1, EGFR mutations, NSCLC" @default.
- W2806760957 created "2018-06-13" @default.
- W2806760957 creator A5004270683 @default.
- W2806760957 creator A5008491549 @default.
- W2806760957 creator A5009012652 @default.
- W2806760957 creator A5011671476 @default.
- W2806760957 creator A5022499603 @default.
- W2806760957 creator A5030691366 @default.
- W2806760957 creator A5046891366 @default.
- W2806760957 creator A5058292168 @default.
- W2806760957 creator A5069860538 @default.
- W2806760957 creator A5071701211 @default.
- W2806760957 date "2018-06-01" @default.
- W2806760957 modified "2023-10-04" @default.
- W2806760957 title "LncRNAs and <em>EGFRvIII</em> sequestered in TEPs enable blood-based NSCLC diagnosis" @default.
- W2806760957 cites W130099911 @default.
- W2806760957 cites W1507361162 @default.
- W2806760957 cites W1537177384 @default.
- W2806760957 cites W1567896933 @default.
- W2806760957 cites W1851952311 @default.
- W2806760957 cites W1868198369 @default.
- W2806760957 cites W1968197443 @default.
- W2806760957 cites W1974035668 @default.
- W2806760957 cites W1978884144 @default.
- W2806760957 cites W1985033899 @default.
- W2806760957 cites W1998441943 @default.
- W2806760957 cites W2004006640 @default.
- W2806760957 cites W2014484261 @default.
- W2806760957 cites W2030596316 @default.
- W2806760957 cites W2062625397 @default.
- W2806760957 cites W2068132555 @default.
- W2806760957 cites W2069774245 @default.
- W2806760957 cites W2081128658 @default.
- W2806760957 cites W2089135124 @default.
- W2806760957 cites W2089956125 @default.
- W2806760957 cites W2102723548 @default.
- W2806760957 cites W2109130949 @default.
- W2806760957 cites W2109225342 @default.
- W2806760957 cites W2111662961 @default.
- W2806760957 cites W2112848779 @default.
- W2806760957 cites W2116168949 @default.
- W2806760957 cites W2119488140 @default.
- W2806760957 cites W2132157071 @default.
- W2806760957 cites W2141410747 @default.
- W2806760957 cites W2141569879 @default.
- W2806760957 cites W2142767404 @default.
- W2806760957 cites W2143859186 @default.
- W2806760957 cites W2148629421 @default.
- W2806760957 cites W2148874508 @default.
- W2806760957 cites W2157820287 @default.
- W2806760957 cites W2159007127 @default.
- W2806760957 cites W2161817637 @default.
- W2806760957 cites W2221837786 @default.
- W2806760957 cites W2427038222 @default.
- W2806760957 cites W2544979428 @default.
- W2806760957 cites W2731334242 @default.
- W2806760957 cites W2981408405 @default.
- W2806760957 doi "https://doi.org/10.2147/cmar.s164227" @default.
- W2806760957 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5997181" @default.
- W2806760957 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29922089" @default.
- W2806760957 hasPublicationYear "2018" @default.
- W2806760957 type Work @default.
- W2806760957 sameAs 2806760957 @default.
- W2806760957 citedByCount "35" @default.
- W2806760957 countsByYear W28067609572018 @default.
- W2806760957 countsByYear W28067609572019 @default.
- W2806760957 countsByYear W28067609572020 @default.
- W2806760957 countsByYear W28067609572021 @default.
- W2806760957 countsByYear W28067609572022 @default.
- W2806760957 countsByYear W28067609572023 @default.
- W2806760957 crossrefType "journal-article" @default.
- W2806760957 hasAuthorship W2806760957A5004270683 @default.
- W2806760957 hasAuthorship W2806760957A5008491549 @default.
- W2806760957 hasAuthorship W2806760957A5009012652 @default.
- W2806760957 hasAuthorship W2806760957A5011671476 @default.
- W2806760957 hasAuthorship W2806760957A5022499603 @default.
- W2806760957 hasAuthorship W2806760957A5030691366 @default.
- W2806760957 hasAuthorship W2806760957A5046891366 @default.
- W2806760957 hasAuthorship W2806760957A5058292168 @default.
- W2806760957 hasAuthorship W2806760957A5069860538 @default.
- W2806760957 hasAuthorship W2806760957A5071701211 @default.
- W2806760957 hasBestOaLocation W28067609571 @default.
- W2806760957 hasConcept C121608353 @default.
- W2806760957 hasConcept C126322002 @default.
- W2806760957 hasConcept C142724271 @default.
- W2806760957 hasConcept C143998085 @default.
- W2806760957 hasConcept C2775934546 @default.
- W2806760957 hasConcept C2776256026 @default.
- W2806760957 hasConcept C502942594 @default.
- W2806760957 hasConcept C58471807 @default.
- W2806760957 hasConcept C71924100 @default.
- W2806760957 hasConcept C89560881 @default.
- W2806760957 hasConceptScore W2806760957C121608353 @default.
- W2806760957 hasConceptScore W2806760957C126322002 @default.
- W2806760957 hasConceptScore W2806760957C142724271 @default.
- W2806760957 hasConceptScore W2806760957C143998085 @default.
- W2806760957 hasConceptScore W2806760957C2775934546 @default.
- W2806760957 hasConceptScore W2806760957C2776256026 @default.