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• W2806793931 abstract "Chromatin immunoprecipitation (ChIP) is a powerful technique allowing for investigation of protein-DNA interactions in living cells. Here, we provide a detailed step-by-step protocol for ChIP and highlight important considerations, challenges and pitfalls often encountered in the ChIP procedure. Furthermore, we present data of key quality control (QC) steps and exemplify material performance validation on transcription factor ChIP to provide a QC guide for setting up ChIP. Finally, we provide guidelines for scaling of the ChIP procedure to ChIP sequencing (ChIP-seq) and discuss important considerations associated with this." @default.
• W2806793931 created "2018-06-13" @default.
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• W2806793931 date "2018-01-01" @default.
• W2806793931 modified "2023-09-27" @default.
• W2806793931 title "Chromatin Immunoprecipitation for Identification of Protein–DNA Interactions in Human Cells" @default.
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• W2806793931 doi "https://doi.org/10.1007/978-1-4939-7871-7_24" @default.
• W2806793931 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29855970" @default.
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