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• W2806984841 abstract "Significance Efficient manufacturing is critical for the translation of cell-based therapies to clinical applications. To date, high-yield expansion of human pluripotent stem cells (hPSC) in suspension bioreactors has not been reported. Here, we present a strategy to shift suspension-grown hPSC to a high-yield state without compromising their ability to differentiate to all three germ layers. In this new state, hPSC expand to densities 5.7 ± 0.2-fold higher than conventional hPSC each passage in suspension bioreactors. High-density suspension cultures enable process intensification, cost reduction, and more efficient manufacturing. This work advances cell-state engineering as a valuable tool to overcome current challenges in therapeutic cell production and processing." @default.
• W2806984841 created "2018-06-13" @default.
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• W2806984841 date "2018-06-04" @default.
• W2806984841 modified "2023-09-28" @default.
• W2806984841 title "Modulating cell state to enhance suspension expansion of human pluripotent stem cells" @default.
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• W2806984841 doi "https://doi.org/10.1073/pnas.1714099115" @default.
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