Matches in SemOpenAlex for { <https://semopenalex.org/work/W2807156594> ?p ?o ?g. }
- W2807156594 abstract "There is an urgent need to assess the effect of anthropogenic chemicals on model cells prior to their release, helping to predict their potential impact on the environment and human health. Laser scanning confocal microscopy (LSCM) and atomic force microscopy (AFM) have each provided an abundance of information on cell physiology. In addition to determining surface architecture, AFM in quantitative imaging (QI) mode probes surface biochemistry and cellular mechanics using minimal applied force, while LSCM offers a window into the cell for imaging fluorescently tagged macromolecules. Correlative AFM-LSCM produces complimentary information on different cellular characteristics for a comprehensive picture of cellular behaviour. We present a correlative AFM-QI-LSCM assay for the simultaneous real-time imaging of living cells in situ, producing multiplexed data on cell morphology and mechanics, surface adhesion and ultrastructure, and real-time localization of multiple fluorescently tagged macromolecules. To demonstrate the broad applicability of this method for disparate cell types, we show altered surface properties, internal molecular arrangement and oxidative stress in model bacterial, fungal and human cells exposed to 2,4-dichlorophenoxyacetic acid. AFM-QI-LSCM is broadly applicable to a variety of cell types and can be used to assess the impact of any multitude of contaminants, alone or in combination." @default.
- W2807156594 created "2018-06-13" @default.
- W2807156594 creator A5020545296 @default.
- W2807156594 creator A5020619064 @default.
- W2807156594 creator A5055839407 @default.
- W2807156594 creator A5065681168 @default.
- W2807156594 creator A5068580952 @default.
- W2807156594 creator A5077591465 @default.
- W2807156594 date "2018-05-29" @default.
- W2807156594 modified "2023-10-10" @default.
- W2807156594 title "Correlative atomic force microscopy quantitative imaging-laser scanning confocal microscopy quantifies the impact of stressors on live cells in real-time" @default.
- W2807156594 cites W1488561686 @default.
- W2807156594 cites W1954977260 @default.
- W2807156594 cites W1969453487 @default.
- W2807156594 cites W1971434563 @default.
- W2807156594 cites W1972624067 @default.
- W2807156594 cites W1980613333 @default.
- W2807156594 cites W1988021279 @default.
- W2807156594 cites W1992902248 @default.
- W2807156594 cites W1994365235 @default.
- W2807156594 cites W1995565650 @default.
- W2807156594 cites W1996426210 @default.
- W2807156594 cites W1997920853 @default.
- W2807156594 cites W1999234315 @default.
- W2807156594 cites W2001560639 @default.
- W2807156594 cites W2005149112 @default.
- W2807156594 cites W2005343502 @default.
- W2807156594 cites W2007971802 @default.
- W2807156594 cites W2008711263 @default.
- W2807156594 cites W2016013258 @default.
- W2807156594 cites W2022313171 @default.
- W2807156594 cites W2022759827 @default.
- W2807156594 cites W2024001778 @default.
- W2807156594 cites W2039297713 @default.
- W2807156594 cites W2041018206 @default.
- W2807156594 cites W2042491408 @default.
- W2807156594 cites W2042770554 @default.
- W2807156594 cites W2046460405 @default.
- W2807156594 cites W2053973736 @default.
- W2807156594 cites W2055059614 @default.
- W2807156594 cites W2058036222 @default.
- W2807156594 cites W2075452338 @default.
- W2807156594 cites W2076317623 @default.
- W2807156594 cites W2082285095 @default.
- W2807156594 cites W2083863660 @default.
- W2807156594 cites W2088481667 @default.
- W2807156594 cites W2089154372 @default.
- W2807156594 cites W2089197146 @default.
- W2807156594 cites W2089918749 @default.
- W2807156594 cites W2091911339 @default.
- W2807156594 cites W2092230232 @default.
- W2807156594 cites W2106059993 @default.
- W2807156594 cites W2106294781 @default.
- W2807156594 cites W2110096013 @default.
- W2807156594 cites W2113740367 @default.
- W2807156594 cites W2115786496 @default.
- W2807156594 cites W2120364099 @default.
- W2807156594 cites W2121825908 @default.
- W2807156594 cites W2122341467 @default.
- W2807156594 cites W2126572275 @default.
- W2807156594 cites W2127854161 @default.
- W2807156594 cites W2131437631 @default.
- W2807156594 cites W2134611117 @default.
- W2807156594 cites W2135314348 @default.
- W2807156594 cites W2144082864 @default.
- W2807156594 cites W2149704712 @default.
- W2807156594 cites W2152484471 @default.
- W2807156594 cites W2157947524 @default.
- W2807156594 cites W2187636587 @default.
- W2807156594 cites W2309646133 @default.
- W2807156594 cites W2487052020 @default.
- W2807156594 cites W2509415889 @default.
- W2807156594 cites W2542260424 @default.
- W2807156594 cites W2605776475 @default.
- W2807156594 cites W2609028313 @default.
- W2807156594 cites W2620099204 @default.
- W2807156594 cites W2742621297 @default.
- W2807156594 cites W2745316792 @default.
- W2807156594 cites W2762786825 @default.
- W2807156594 cites W2769172998 @default.
- W2807156594 cites W2793446791 @default.
- W2807156594 cites W2796863313 @default.
- W2807156594 doi "https://doi.org/10.1038/s41598-018-26433-1" @default.
- W2807156594 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5973941" @default.
- W2807156594 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29844489" @default.
- W2807156594 hasPublicationYear "2018" @default.
- W2807156594 type Work @default.
- W2807156594 sameAs 2807156594 @default.
- W2807156594 citedByCount "26" @default.
- W2807156594 countsByYear W28071565942018 @default.
- W2807156594 countsByYear W28071565942019 @default.
- W2807156594 countsByYear W28071565942020 @default.
- W2807156594 countsByYear W28071565942021 @default.
- W2807156594 countsByYear W28071565942022 @default.
- W2807156594 countsByYear W28071565942023 @default.
- W2807156594 crossrefType "journal-article" @default.
- W2807156594 hasAuthorship W2807156594A5020545296 @default.
- W2807156594 hasAuthorship W2807156594A5020619064 @default.
- W2807156594 hasAuthorship W2807156594A5055839407 @default.
- W2807156594 hasAuthorship W2807156594A5065681168 @default.