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• W2807381472 abstract "Denitrification is an indispensable pathway of nitrogen removal in aquatic ecosystems, and plays an important role in decreasing eutrophication induced by excessive reactive nitrogen pollution. Aquatic environments also suffer from antibiotic pollution due to runoff from farms and sewage systems. The aim of this study was to investigate the effect of oxytetracycline stress on denitrifying functional genes, the microbial community and metabolic pathways in sediments using high-throughput sequencing and metagenomic analysis. The oxytetracycline was observed to significantly inhibit the abundance of nirK and nosZ genes (P < 0.001). KEGG pathway annotation indicated that oxytetracycline treatment decreased the abundance of nitrate reductase, nitrite reductase and N2O reductase. Functional annotations revealed that oxytetracycline exposure decreased the abundance of the protein metabolism subsystem in the bacterial community. Metagenomic sequencing demonstrated that the abundance of Proteobacteria and Firmicutes increased with oxytetracycline exposure while the Actinobacteria decreased. In sediments, Pseudomonas and Bradyrhizobium were major contributors to denitrification and oxytetracycline exposure resulted in a decreased abundance of Bradyrhizobium. These results indicated that oxytetracycline residues influences the denitrifier community and may heighten occurrence of reactive nitrogen in aquatic ecosystems." @default.
• W2807381472 created "2018-06-13" @default.
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• W2807381472 date "2018-10-01" @default.
• W2807381472 modified "2023-10-18" @default.
• W2807381472 title "Metagenomic insights into the effect of oxytetracycline on microbial structures, functions and functional genes in sediment denitrification" @default.
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• W2807381472 doi "https://doi.org/10.1016/j.ecoenv.2018.05.045" @default.
• W2807381472 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29870921" @default.
• W2807381472 hasPublicationYear "2018" @default.
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