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• W2807410003 abstract "Iron plays important roles in the growth, reproduction and pathogenicity of Aeromonas hydrophila. In this study, we detected and compared the mRNA and protein expression profiles of A. hydrophila under normal and iron restricted medium with 200 μM 2,2-Dipyridyl using RNA Sequencing (RNA-seq) and isobaric tags for relative and absolute quantification (iTRAQ) analyses. There were 1204 genes (601 up- and 603 down-regulated) and 236 proteins (90 up- and 146 down-regulated) shown to be differentially expressed, and 167 genes and proteins that showed consistent expression. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed that the differentially expressed genes and proteins were mainly involved in iron ion transport, protein activity, energy metabolism and virulence processes. Further validation of the RNA-seq and iTRAQ results by quantitative real-time PCR (qPCR) revealed that 18 of the 20 selected genes were consistently expressed. The iron-ion absorption and concentration of A. hydrophila under iron-limited conditions were enhanced, and most virulence factors (protease activity, hemolytic activity, lipase activity, and swimming ability) were also increased. Artificial A. hydrophila infection caused higher mortality in cyprinid Megalobrama amblycephala under iron-limited conditions. Understanding the responses of pathogenic Aeromonas hydrophila within the hostile environment of the fish host, devoid of free iron, is important to reveal bacterial infection and pathogenesis. This study further confirmed the previous finding that iron-limitation efficiently enhanced the virulence of A. hydrophila using multi-omics analyses. We identified differentially expressed genes and proteins, related to enterobactin synthesis and virulence establishment, that play important roles in addressing iron scarcity." @default.
• W2807410003 created "2018-06-13" @default.
• W2807410003 creator A5000838832 @default.
• W2807410003 creator A5011868343 @default.
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• W2807410003 creator A5068690304 @default.
• W2807410003 creator A5074339191 @default.
• W2807410003 date "2018-06-04" @default.
• W2807410003 modified "2023-09-24" @default.
• W2807410003 title "Comparative transcriptomic and proteomic analyses reveal upregulated expression of virulence and iron transport factors of Aeromonas hydrophila under iron limitation" @default.
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• W2807410003 doi "https://doi.org/10.1186/s12866-018-1178-8" @default.
• W2807410003 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5987420" @default.
• W2807410003 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29866030" @default.
• W2807410003 hasPublicationYear "2018" @default.
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