FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2807740134> ?p ?o ?g. }

• W2807740134 abstract "Abstract Bacteria take up carbohydrates by membrane-integral sugar specific phosphoenolpyruvate-dependent carbohydrate:phosphotransferase systems (PTS). Although PTS is at the heart of bacterial carbon uptake and centrally involved in regulation of carbon metabolism, little is known about localization and putative oligomerization of the permease subunits (EII) of PTS. Here, we analyzed localization of the fructose specific PtsF and the glucose specific PtsG transporters from C. glutamicum using widefield and single molecule localization microscopy. PtsG and PtsF form membrane embedded clusters that localize in a punctate pattern within the cell membrane. The size, number and fluorescence of the observed clusters changes upon presence or absence of the transported substrate. In presence of the transport substrate clusters significantly increased in size. Photo-activated localization microscopy (PALM) data revealed that, in presence of different carbon sources, the number of EII protein events per cluster remain the same, however the density of PTS molecules within a cluster reduces. Our work reveals a simple mechanism for efficient membrane occupancy regulation. Clusters of PTS EII transporters are densely packed in absence of a suitable substrate. In presence of a transport substrate the EII proteins in individual clusters occupy larger membrane areas, thereby decreasing protein density in individual clusters. This mechanism allows for efficient use of the limited membrane space under varying growth conditions without need of protein removal and re-synthesis. Importance The carbohydrate transport system PTS is centrally involved in the regulation of sugar metabolism. Although much is known about the regulatory interaction, the genetic control and the structure/function relationship of the individual PTS components, we know almost nothing about the spatio-temporal organization of the PTS proteins within the cell. We find dynamic clustering of PTS permeases in Corynebacterium glutamicum. Using single molecule resolution photo-activated localization microscopy we could show that PTS EII protein cluster are dynamically changing protein density upon substrate availability. Our findings imply a novel strategy of regulating limited membrane space efficiently. Furthermore, these data will provide important insights in modelling carbohydrate fluxes in cells, since current models assume a homogeneous distribution of PTS permeases within the membrane." @default.
• W2807740134 created "2018-06-21" @default.
• W2807740134 creator A5028088775 @default.
• W2807740134 creator A5030611032 @default.
• W2807740134 creator A5077193755 @default.
• W2807740134 date "2018-06-18" @default.
• W2807740134 modified "2023-09-25" @default.
• W2807740134 title "Substrate-dependent cluster density dynamics in bacterial phosphotransferase system permeases" @default.
• W2807740134 cites W1501727668 @default.
• W2807740134 cites W1523542975 @default.
• W2807740134 cites W1524075358 @default.
• W2807740134 cites W1931147290 @default.
• W2807740134 cites W1969186425 @default.
• W2807740134 cites W1973180388 @default.
• W2807740134 cites W1976861576 @default.
• W2807740134 cites W1977110567 @default.
• W2807740134 cites W1977986827 @default.
• W2807740134 cites W1979581724 @default.
• W2807740134 cites W1985761352 @default.
• W2807740134 cites W1986176620 @default.
• W2807740134 cites W1999439314 @default.
• W2807740134 cites W2036449301 @default.
• W2807740134 cites W2049253186 @default.
• W2807740134 cites W2050436990 @default.
• W2807740134 cites W2057276007 @default.
• W2807740134 cites W2061842603 @default.
• W2807740134 cites W2073923636 @default.
• W2807740134 cites W2088114805 @default.
• W2807740134 cites W2090855137 @default.
• W2807740134 cites W2091775757 @default.
• W2807740134 cites W2094254360 @default.
• W2807740134 cites W2095883429 @default.
• W2807740134 cites W2099630547 @default.
• W2807740134 cites W2104413400 @default.
• W2807740134 cites W2105627149 @default.
• W2807740134 cites W2108528740 @default.
• W2807740134 cites W2111275357 @default.
• W2807740134 cites W2114828024 @default.
• W2807740134 cites W2115714059 @default.
• W2807740134 cites W2115955917 @default.
• W2807740134 cites W2118561262 @default.
• W2807740134 cites W2119831600 @default.
• W2807740134 cites W2121462614 @default.
• W2807740134 cites W2123180594 @default.
• W2807740134 cites W2123697930 @default.
• W2807740134 cites W2129502305 @default.
• W2807740134 cites W2136626347 @default.
• W2807740134 cites W2136700922 @default.
• W2807740134 cites W2142776290 @default.
• W2807740134 cites W2143521363 @default.
• W2807740134 cites W2156804656 @default.
• W2807740134 cites W2156901916 @default.
• W2807740134 cites W2164190876 @default.
• W2807740134 cites W2166374078 @default.
• W2807740134 cites W2167279371 @default.
• W2807740134 cites W2167796588 @default.
• W2807740134 cites W2167874836 @default.
• W2807740134 cites W2403232642 @default.
• W2807740134 cites W2492885664 @default.
• W2807740134 cites W2501938907 @default.
• W2807740134 cites W2551533810 @default.
• W2807740134 cites W2588179750 @default.
• W2807740134 cites W2598405591 @default.
• W2807740134 cites W2804136912 @default.
• W2807740134 cites W3023958910 @default.
• W2807740134 doi "https://doi.org/10.1101/349514" @default.
• W2807740134 hasPublicationYear "2018" @default.
• W2807740134 type Work @default.
• W2807740134 sameAs 2807740134 @default.
• W2807740134 citedByCount "0" @default.
• W2807740134 crossrefType "posted-content" @default.
• W2807740134 hasAuthorship W2807740134A5028088775 @default.
• W2807740134 hasAuthorship W2807740134A5030611032 @default.
• W2807740134 hasAuthorship W2807740134A5077193755 @default.
• W2807740134 hasBestOaLocation W28077401341 @default.
• W2807740134 hasConcept C104317684 @default.
• W2807740134 hasConcept C115335371 @default.
• W2807740134 hasConcept C12554922 @default.
• W2807740134 hasConcept C143937172 @default.
• W2807740134 hasConcept C144647389 @default.
• W2807740134 hasConcept C149011108 @default.
• W2807740134 hasConcept C161200384 @default.
• W2807740134 hasConcept C181199279 @default.
• W2807740134 hasConcept C185592680 @default.
• W2807740134 hasConcept C18903297 @default.
• W2807740134 hasConcept C190114821 @default.
• W2807740134 hasConcept C2777289219 @default.
• W2807740134 hasConcept C41625074 @default.
• W2807740134 hasConcept C45656491 @default.
• W2807740134 hasConcept C55493867 @default.
• W2807740134 hasConcept C86803240 @default.
• W2807740134 hasConceptScore W2807740134C104317684 @default.
• W2807740134 hasConceptScore W2807740134C115335371 @default.
• W2807740134 hasConceptScore W2807740134C12554922 @default.
• W2807740134 hasConceptScore W2807740134C143937172 @default.
• W2807740134 hasConceptScore W2807740134C144647389 @default.
• W2807740134 hasConceptScore W2807740134C149011108 @default.
• W2807740134 hasConceptScore W2807740134C161200384 @default.
• W2807740134 hasConceptScore W2807740134C181199279 @default.
• W2807740134 hasConceptScore W2807740134C185592680 @default.

• next