FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2807912286> ?p ?o ?g. }

• W2807912286 abstract "The strain Y1, with a notably high production of neutral protease, was isolated from naturally fermented broad beans and subsequently identified as Aspergillus oryzae, through the analysis of its morphology characteristics and 18S rDNA sequence. Naturally fermented broad beans are the main raw material in Sichuan broad-bean sauce. The neutral protease from Aspergillus oryzae Y1 was purified using ammonium sulphate precipitation and DEAE-Sepharose Fast Flow chromatography, which resulted in a 10.0-fold increase in the specific activity (2264.3 U/mg) and a recovery rate of 21%. The estimated molecular mass of the purified protease was approximately 45 kDa. The optimal pH and temperature of the purified protease were 7.0 and 55 °C, respectively. The heat resistance of the purified protease was significantly higher than the commercial protease. The effect of metal ions on the activity of the purified protease approximated that of commercial neutral protease. Furthermore, the maximum hydrolysis rate (Vmax) and apparent Michaelis–Menten constant (Km) values of the purified protease were 256.4103 μg/mL min and 20.0769 mg/mL, respectively. The purified protease had a higher affinity for the substrate than the commercial neutral protease. All the results suggest that this neutral protease exhibits the potential for application in industry due to its good resistance to high temperatures and wide range of acids and bases." @default.
• W2807912286 created "2018-06-21" @default.
• W2807912286 creator A5003753200 @default.
• W2807912286 creator A5009702089 @default.
• W2807912286 creator A5016496430 @default.
• W2807912286 creator A5021261036 @default.
• W2807912286 creator A5027064196 @default.
• W2807912286 creator A5032650507 @default.
• W2807912286 creator A5047471358 @default.
• W2807912286 creator A5066842627 @default.
• W2807912286 creator A5069458481 @default.
• W2807912286 creator A5084445575 @default.
• W2807912286 date "2018-06-12" @default.
• W2807912286 modified "2023-10-18" @default.
• W2807912286 title "Purification and characterization of neutral protease from Aspergillus oryzae Y1 isolated from naturally fermented broad beans" @default.
• W2807912286 cites W1170487763 @default.
• W2807912286 cites W1824325467 @default.
• W2807912286 cites W1966034241 @default.
• W2807912286 cites W1976039532 @default.
• W2807912286 cites W1979655538 @default.
• W2807912286 cites W1983947920 @default.
• W2807912286 cites W1985090646 @default.
• W2807912286 cites W1987467679 @default.
• W2807912286 cites W1997234557 @default.
• W2807912286 cites W1999092946 @default.
• W2807912286 cites W2001049522 @default.
• W2807912286 cites W2008057474 @default.
• W2807912286 cites W2015321942 @default.
• W2807912286 cites W2015506170 @default.
• W2807912286 cites W2019688356 @default.
• W2807912286 cites W2020994501 @default.
• W2807912286 cites W2026003146 @default.
• W2807912286 cites W2028062487 @default.
• W2807912286 cites W2036956907 @default.
• W2807912286 cites W2039864978 @default.
• W2807912286 cites W2054184938 @default.
• W2807912286 cites W2057835264 @default.
• W2807912286 cites W2072163445 @default.
• W2807912286 cites W2073717097 @default.
• W2807912286 cites W2078262420 @default.
• W2807912286 cites W2084336767 @default.
• W2807912286 cites W2085074262 @default.
• W2807912286 cites W2089173136 @default.
• W2807912286 cites W2093344889 @default.
• W2807912286 cites W2102843616 @default.
• W2807912286 cites W2118980542 @default.
• W2807912286 cites W2199475639 @default.
• W2807912286 cites W2404390622 @default.
• W2807912286 cites W2410578887 @default.
• W2807912286 cites W2460111973 @default.
• W2807912286 cites W2512794384 @default.
• W2807912286 cites W2547354888 @default.
• W2807912286 cites W2600695776 @default.
• W2807912286 cites W2612347569 @default.
• W2807912286 cites W2615103467 @default.
• W2807912286 cites W990455329 @default.
• W2807912286 doi "https://doi.org/10.1186/s13568-018-0611-6" @default.
• W2807912286 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5997607" @default.
• W2807912286 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29896640" @default.
• W2807912286 hasPublicationYear "2018" @default.
• W2807912286 type Work @default.
• W2807912286 sameAs 2807912286 @default.
• W2807912286 citedByCount "26" @default.
• W2807912286 countsByYear W28079122862018 @default.
• W2807912286 countsByYear W28079122862019 @default.
• W2807912286 countsByYear W28079122862020 @default.
• W2807912286 countsByYear W28079122862021 @default.
• W2807912286 countsByYear W28079122862022 @default.
• W2807912286 countsByYear W28079122862023 @default.
• W2807912286 crossrefType "journal-article" @default.
• W2807912286 hasAuthorship W2807912286A5003753200 @default.
• W2807912286 hasAuthorship W2807912286A5009702089 @default.
• W2807912286 hasAuthorship W2807912286A5016496430 @default.
• W2807912286 hasAuthorship W2807912286A5021261036 @default.
• W2807912286 hasAuthorship W2807912286A5027064196 @default.
• W2807912286 hasAuthorship W2807912286A5032650507 @default.
• W2807912286 hasAuthorship W2807912286A5047471358 @default.
• W2807912286 hasAuthorship W2807912286A5066842627 @default.
• W2807912286 hasAuthorship W2807912286A5069458481 @default.
• W2807912286 hasAuthorship W2807912286A5084445575 @default.
• W2807912286 hasBestOaLocation W28079122861 @default.
• W2807912286 hasConcept C100544194 @default.
• W2807912286 hasConcept C181199279 @default.
• W2807912286 hasConcept C185592680 @default.
• W2807912286 hasConcept C2776714187 @default.
• W2807912286 hasConcept C2781204770 @default.
• W2807912286 hasConcept C31903555 @default.
• W2807912286 hasConcept C43617362 @default.
• W2807912286 hasConcept C55493867 @default.
• W2807912286 hasConcept C58121356 @default.
• W2807912286 hasConcept C86803240 @default.
• W2807912286 hasConcept C94412978 @default.
• W2807912286 hasConceptScore W2807912286C100544194 @default.
• W2807912286 hasConceptScore W2807912286C181199279 @default.
• W2807912286 hasConceptScore W2807912286C185592680 @default.
• W2807912286 hasConceptScore W2807912286C2776714187 @default.
• W2807912286 hasConceptScore W2807912286C2781204770 @default.
• W2807912286 hasConceptScore W2807912286C31903555 @default.
• W2807912286 hasConceptScore W2807912286C43617362 @default.
• W2807912286 hasConceptScore W2807912286C55493867 @default.

• next