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- W2807924894 abstract "The fabrication of a novel electrochemical immunosensor for rapid and precise determination of prolactin was carried out using carbon paste electrode (CPE) consist of ionic liquid (IL) and graphite. Gold nanoparticles were employed as a modifier on the surface of CPE to immobilize the prolactin antibody (anti-PRL). The immunoassay was set up by sandwiching the antigen between prolactin antibody and the polyclonal anti-human-prolactin antibody labeled with horseradish peroxidase (HRP-labeled anti-PRL) as secondary antibody, on the surface of modified CPE. The reaction between O-aminophenol (OAP) and H2O2 which is catalyzed by labeled HRP on the sandwich immunosensor generate a signal in differential pulse voltammetry (DPV) that is used to determine the concentration of prolactin. This immunosensor provides the measurement of prolactin concentration in a linear range of 25.0–2000.0 mIU L−1 with a detection limit 12.5 mIU L−1. Moreover, it is applicable in the clinical assay of prolactin due to its high sensitivity and acceptable stability." @default.
- W2807924894 created "2018-06-21" @default.
- W2807924894 creator A5056486522 @default.
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- W2807924894 date "2018-10-01" @default.
- W2807924894 modified "2023-09-27" @default.
- W2807924894 title "Amperometric immunosensor for prolactin hormone measurement using antibodies loaded on a nano-Au monolayer modified ionic liquid carbon paste electrode" @default.
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- W2807924894 doi "https://doi.org/10.1016/j.talanta.2018.06.044" @default.
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