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- W2807990110 abstract "This method provides a fast approach for the determination of plasma membrane partitioning of any fluorescently-tagged peripherally-associated protein using the profiles of fluorescence intensity across the plasma membrane. Measured fluorescence profiles are fitted by a model for membrane and cytoplasm fluorescence distribution along a line applied perpendicularly to the cell periphery. This model is constructed from the fluorescence intensity values in reference cells expressing a fluorescently-tagged marker for cytoplasm and with FM 4-64-labeled plasma membrane. The method can be applied to various cell types and organisms; however, only plasma membranes of non-neighboring cells can be evaluated. This fast microscopy-based method is suitable for experiments, where subtle and dynamic changes of plasma membrane-associated markers are expected and need to be quantified, e.g., in the analysis of mutant versions of proteins, inhibitor treatments, and signal transduction observations. The method is implemented in a multi-platform R package that is coupled with an ImageJ macro that serves as a user-friendly interface." @default.
- W2807990110 created "2018-06-21" @default.
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- W2807990110 date "2018-06-15" @default.
- W2807990110 modified "2023-09-26" @default.
- W2807990110 title "Determination of Plasma Membrane Partitioning for Peripherally-associated Proteins" @default.
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- W2807990110 doi "https://doi.org/10.3791/57837" @default.
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