FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2808021566> ?p ?o ?g. }

• W2808021566 endingPage "2314" @default.
• W2808021566 startingPage "2305" @default.
• W2808021566 abstract "Abstract The oxygenic photosynthetic bacterium Synechocystis sp. PCC 6803 (S6803) is a model cyanobacterium widely used for fundamental research and biotechnology applications. Due to its polyploidy, existing methods for genome engineering of S6803 require multiple rounds of selection to modify all genome copies, which is time‐consuming and inefficient. In this study, we engineered the Cas9 tool for one‐step, segregation‐free genome engineering. We further used our Cas9 tool to delete three of seven S6803 native plasmids. Our results show that all three small‐size native plasmids, but not the large‐size native plasmids, can be deleted with this tool. To further facilitate heterologous gene expression in S6803, a shuttle vector based on the native plasmid pCC5.2 was created. The shuttle vector can be introduced into Cas9‐containing S6803 in one step without requiring segregation and can be stably maintained without antibiotic pressure for at least 30 days. Moreover, genes encoded on the shuttle vector remain functional after 30 days of continuous cultivation without selective pressure. Thus, this study provides a set of new tools for rapid modification of the S6803 genome and for stable expression of heterologous genes, potentially facilitating both fundamental research and biotechnology applications using S6803." @default.
• W2808021566 created "2018-06-21" @default.
• W2808021566 creator A5008156184 @default.
• W2808021566 creator A5013799944 @default.
• W2808021566 creator A5025437312 @default.
• W2808021566 creator A5030281278 @default.
• W2808021566 creator A5035403159 @default.
• W2808021566 creator A5065869083 @default.
• W2808021566 creator A5074036155 @default.
• W2808021566 creator A5086276509 @default.
• W2808021566 date "2018-06-29" @default.
• W2808021566 modified "2023-10-14" @default.
• W2808021566 title "Developing a Cas9‐based tool to engineer native plasmids in <i>Synechocystis</i> sp. PCC 6803" @default.
• W2808021566 cites W1486208928 @default.
• W2808021566 cites W1783450596 @default.
• W2808021566 cites W2000046950 @default.
• W2808021566 cites W2002669907 @default.
• W2808021566 cites W2004541440 @default.
• W2808021566 cites W2015555968 @default.
• W2808021566 cites W2016667239 @default.
• W2808021566 cites W2029218099 @default.
• W2808021566 cites W2040912668 @default.
• W2808021566 cites W2053512440 @default.
• W2808021566 cites W2068219766 @default.
• W2808021566 cites W2073317899 @default.
• W2808021566 cites W2085679883 @default.
• W2808021566 cites W2107523606 @default.
• W2808021566 cites W2111332150 @default.
• W2808021566 cites W2128556638 @default.
• W2808021566 cites W2129741035 @default.
• W2808021566 cites W2133248262 @default.
• W2808021566 cites W2160051211 @default.
• W2808021566 cites W2182456467 @default.
• W2808021566 cites W2203862394 @default.
• W2808021566 cites W2470889463 @default.
• W2808021566 cites W2527387613 @default.
• W2808021566 cites W2563521043 @default.
• W2808021566 cites W2610391087 @default.
• W2808021566 cites W2734284759 @default.
• W2808021566 cites W2740041002 @default.
• W2808021566 cites W2751757223 @default.
• W2808021566 cites W2765214919 @default.
• W2808021566 cites W2768722917 @default.
• W2808021566 cites W2781510265 @default.
• W2808021566 cites W37174705 @default.
• W2808021566 cites W414350953 @default.
• W2808021566 doi "https://doi.org/10.1002/bit.26747" @default.
• W2808021566 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29896914" @default.
• W2808021566 hasPublicationYear "2018" @default.
• W2808021566 type Work @default.
• W2808021566 sameAs 2808021566 @default.
• W2808021566 citedByCount "26" @default.
• W2808021566 countsByYear W28080215662018 @default.
• W2808021566 countsByYear W28080215662019 @default.
• W2808021566 countsByYear W28080215662020 @default.
• W2808021566 countsByYear W28080215662021 @default.
• W2808021566 countsByYear W28080215662022 @default.
• W2808021566 countsByYear W28080215662023 @default.
• W2808021566 crossrefType "journal-article" @default.
• W2808021566 hasAuthorship W2808021566A5008156184 @default.
• W2808021566 hasAuthorship W2808021566A5013799944 @default.
• W2808021566 hasAuthorship W2808021566A5025437312 @default.
• W2808021566 hasAuthorship W2808021566A5030281278 @default.
• W2808021566 hasAuthorship W2808021566A5035403159 @default.
• W2808021566 hasAuthorship W2808021566A5065869083 @default.
• W2808021566 hasAuthorship W2808021566A5074036155 @default.
• W2808021566 hasAuthorship W2808021566A5086276509 @default.
• W2808021566 hasConcept C104317684 @default.
• W2808021566 hasConcept C132455925 @default.
• W2808021566 hasConcept C141231307 @default.
• W2808021566 hasConcept C143065580 @default.
• W2808021566 hasConcept C144501496 @default.
• W2808021566 hasConcept C145290725 @default.
• W2808021566 hasConcept C145640855 @default.
• W2808021566 hasConcept C191908910 @default.
• W2808021566 hasConcept C22744801 @default.
• W2808021566 hasConcept C2780115692 @default.
• W2808021566 hasConcept C2780238584 @default.
• W2808021566 hasConcept C40767141 @default.
• W2808021566 hasConcept C54355233 @default.
• W2808021566 hasConcept C70721500 @default.
• W2808021566 hasConcept C86803240 @default.
• W2808021566 hasConcept C92087593 @default.
• W2808021566 hasConcept C98108389 @default.
• W2808021566 hasConceptScore W2808021566C104317684 @default.
• W2808021566 hasConceptScore W2808021566C132455925 @default.
• W2808021566 hasConceptScore W2808021566C141231307 @default.
• W2808021566 hasConceptScore W2808021566C143065580 @default.
• W2808021566 hasConceptScore W2808021566C144501496 @default.
• W2808021566 hasConceptScore W2808021566C145290725 @default.
• W2808021566 hasConceptScore W2808021566C145640855 @default.
• W2808021566 hasConceptScore W2808021566C191908910 @default.
• W2808021566 hasConceptScore W2808021566C22744801 @default.
• W2808021566 hasConceptScore W2808021566C2780115692 @default.
• W2808021566 hasConceptScore W2808021566C2780238584 @default.
• W2808021566 hasConceptScore W2808021566C40767141 @default.
• W2808021566 hasConceptScore W2808021566C54355233 @default.
• W2808021566 hasConceptScore W2808021566C70721500 @default.

• next