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• W2808075666 abstract "Loss-of-function studies are critically important in gene functional analysis of model organisms and cells. However, conditional gene inactivation in diploid cells is difficult to achieve, as it involves laborious vector construction, multifold electroporation, and complicated genotyping. Here, a strategy is presented for generating biallelic conditional gene and DNA regulatory region knockouts in mouse embryonic stem cells by codelivery of CRISPR-Cas9 and short-homology-arm targeting vectors sequentially or simultaneously. Collectively, a simple and rapid method was presented to knock out any DNA element conditionally. This approach will facilitate the functional studies of essential genes and regulatory regions during development." @default.
• W2808075666 created "2018-06-21" @default.
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• W2808075666 date "2018-06-14" @default.
• W2808075666 modified "2023-09-30" @default.
• W2808075666 title "User-Friendly Genetic Conditional Knockout Strategies by CRISPR/Cas9" @default.
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• W2808075666 doi "https://doi.org/10.1155/2018/9576959" @default.
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