Matches in SemOpenAlex for { <https://semopenalex.org/work/W2808201997> ?p ?o ?g. }
- W2808201997 endingPage "2256" @default.
- W2808201997 startingPage "2241" @default.
- W2808201997 abstract "Biosynthesis of the azasugar 1-deoxynojirimycin (DNJ) critically involves a transamination in the first committed step. Here, we identify the azasugar biosynthetic cluster signature in Paenibacillus polymyxa SC2 (Ppo), homologous to that reported in Bacillus amyloliquefaciens FZB42 (Bam), and report the characterization of the aminotransferase GabT1 (named from Bam). GabT1 from Ppo exhibits a specific activity of 4.9 nmol/min/mg at 30°C (pH 7.5), a somewhat promiscuous amino donor selectivity, and curvilinear steady-state kinetics that do not reflect the predicted ping-pong behavior typical of aminotransferases. Analysis of the first half reaction with l-glutamate in the absence of the acceptor fructose 6-phosphate revealed that it was capable of catalyzing multiple turnovers of glutamate. Kinetic modeling of steady-state initial velocity data was consistent with a novel hybrid branching kinetic mechanism which included dissociation of PMP after the first half reaction to generate the apoenzyme which could bind PLP for another catalytic deamination event. Based on comparative sequence analyses, we identified an uncommon His-Val dyad in the PLP-binding pocket which we hypothesized was responsible for the unusual kinetics. Restoration of the conserved PLP-binding site motif via the mutant H119F restored classic ping-pong kinetic behavior." @default.
- W2808201997 created "2018-06-21" @default.
- W2808201997 creator A5036314386 @default.
- W2808201997 creator A5063185042 @default.
- W2808201997 date "2018-07-16" @default.
- W2808201997 modified "2023-09-26" @default.
- W2808201997 title "Characterization of the PLP-dependent transaminase initiating azasugar biosynthesis" @default.
- W2808201997 cites W1508136483 @default.
- W2808201997 cites W1580746914 @default.
- W2808201997 cites W1873468772 @default.
- W2808201997 cites W1963650624 @default.
- W2808201997 cites W1967999247 @default.
- W2808201997 cites W1972151960 @default.
- W2808201997 cites W1973389245 @default.
- W2808201997 cites W1979635562 @default.
- W2808201997 cites W1984546214 @default.
- W2808201997 cites W1989916228 @default.
- W2808201997 cites W1991944881 @default.
- W2808201997 cites W1994334199 @default.
- W2808201997 cites W1996829379 @default.
- W2808201997 cites W2004650695 @default.
- W2808201997 cites W2018849208 @default.
- W2808201997 cites W2022524141 @default.
- W2808201997 cites W2022961858 @default.
- W2808201997 cites W2031200361 @default.
- W2808201997 cites W2031860944 @default.
- W2808201997 cites W2033735656 @default.
- W2808201997 cites W2040995525 @default.
- W2808201997 cites W2048161430 @default.
- W2808201997 cites W2054159133 @default.
- W2808201997 cites W2057987155 @default.
- W2808201997 cites W2063665475 @default.
- W2808201997 cites W2067338816 @default.
- W2808201997 cites W2069482173 @default.
- W2808201997 cites W2077381233 @default.
- W2808201997 cites W2077723497 @default.
- W2808201997 cites W2079639372 @default.
- W2808201997 cites W2084619555 @default.
- W2808201997 cites W2085285145 @default.
- W2808201997 cites W2089530500 @default.
- W2808201997 cites W2094902573 @default.
- W2808201997 cites W2103351205 @default.
- W2808201997 cites W2110094991 @default.
- W2808201997 cites W2111066410 @default.
- W2808201997 cites W2116919278 @default.
- W2808201997 cites W2118707645 @default.
- W2808201997 cites W2119683617 @default.
- W2808201997 cites W2123326337 @default.
- W2808201997 cites W2125184044 @default.
- W2808201997 cites W2125868974 @default.
- W2808201997 cites W2148828869 @default.
- W2808201997 cites W2150549254 @default.
- W2808201997 cites W2159103092 @default.
- W2808201997 cites W2164045406 @default.
- W2808201997 cites W2319751463 @default.
- W2808201997 cites W2529420624 @default.
- W2808201997 cites W2544811605 @default.
- W2808201997 cites W2950699433 @default.
- W2808201997 cites W3026590205 @default.
- W2808201997 cites W39209531 @default.
- W2808201997 cites W4301020220 @default.
- W2808201997 cites W64499177 @default.
- W2808201997 cites W74666171 @default.
- W2808201997 cites W2050453576 @default.
- W2808201997 doi "https://doi.org/10.1042/bcj20180340" @default.
- W2808201997 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29907615" @default.
- W2808201997 hasPublicationYear "2018" @default.
- W2808201997 type Work @default.
- W2808201997 sameAs 2808201997 @default.
- W2808201997 citedByCount "5" @default.
- W2808201997 countsByYear W28082019972021 @default.
- W2808201997 countsByYear W28082019972022 @default.
- W2808201997 countsByYear W28082019972023 @default.
- W2808201997 crossrefType "journal-article" @default.
- W2808201997 hasAuthorship W2808201997A5036314386 @default.
- W2808201997 hasAuthorship W2808201997A5063185042 @default.
- W2808201997 hasConcept C121332964 @default.
- W2808201997 hasConcept C148898269 @default.
- W2808201997 hasConcept C181199279 @default.
- W2808201997 hasConcept C185592680 @default.
- W2808201997 hasConcept C2776877979 @default.
- W2808201997 hasConcept C2778886173 @default.
- W2808201997 hasConcept C41183919 @default.
- W2808201997 hasConcept C553450214 @default.
- W2808201997 hasConcept C55493867 @default.
- W2808201997 hasConcept C56856141 @default.
- W2808201997 hasConcept C62520636 @default.
- W2808201997 hasConcept C68793091 @default.
- W2808201997 hasConcept C71240020 @default.
- W2808201997 hasConcept C86803240 @default.
- W2808201997 hasConceptScore W2808201997C121332964 @default.
- W2808201997 hasConceptScore W2808201997C148898269 @default.
- W2808201997 hasConceptScore W2808201997C181199279 @default.
- W2808201997 hasConceptScore W2808201997C185592680 @default.
- W2808201997 hasConceptScore W2808201997C2776877979 @default.
- W2808201997 hasConceptScore W2808201997C2778886173 @default.
- W2808201997 hasConceptScore W2808201997C41183919 @default.
- W2808201997 hasConceptScore W2808201997C553450214 @default.