Matches in SemOpenAlex for { <https://semopenalex.org/work/W2808297243> ?p ?o ?g. }
- W2808297243 endingPage "94" @default.
- W2808297243 startingPage "84" @default.
- W2808297243 abstract "Laccases, multicopper oxidoreductases, are mainly produced in white-rot fungi and are considered as ideal green catalysts in industrial and biotechnological applications. However, the development of laccases is limited due to the slow growth of natural laccase producing strains and the low expression levels of laccases. In this study, we designed three regulation strategies for laccase gene expression in the model fungus Aspergillus nidulans. By introducing various promoters in front of the laccase gene pslcc from the white-rot fungus Pycnoporus sanguineus, we found that the laccase gene with the original promoter had effective expression in A. nidulans. Using the previously identified transcription factor RsmA regulatory mechanism, the aflR promoter was inserted into the pslcc expression vectors, and the laccase production was 15-fold higher in the strain overexpressing of RsmA compared to the control strain. To improve the laccase yield, the dipeptidyl-peptidase DppV, aspartic protease PepA and mannosyltransferase Mnn9 were successfully deleted in the A. nidulans host. The laccase activities were increased approximately 8-fold and 13-fold in the double deletions strains of Δmnn9ΔpepA and ΔdppVΔpepA over the control strains, respectively. Taken together, these results not only demonstrate an efficient system for heterologous protein production in the model fungus A. nidulans but also provide a general approach to applying regulatory methods to control gene expression." @default.
- W2808297243 created "2018-06-21" @default.
- W2808297243 creator A5000432967 @default.
- W2808297243 creator A5018592150 @default.
- W2808297243 creator A5058910088 @default.
- W2808297243 creator A5087785658 @default.
- W2808297243 date "2018-06-12" @default.
- W2808297243 modified "2023-09-29" @default.
- W2808297243 title "Rational design for fungal laccase production in the model host Aspergillus nidulans" @default.
- W2808297243 cites W1592766146 @default.
- W2808297243 cites W1672187802 @default.
- W2808297243 cites W1847255561 @default.
- W2808297243 cites W1903107055 @default.
- W2808297243 cites W1966282114 @default.
- W2808297243 cites W1967649036 @default.
- W2808297243 cites W1969742766 @default.
- W2808297243 cites W1973595596 @default.
- W2808297243 cites W1980299910 @default.
- W2808297243 cites W1981579563 @default.
- W2808297243 cites W1995186669 @default.
- W2808297243 cites W1999003074 @default.
- W2808297243 cites W2002760953 @default.
- W2808297243 cites W2008775392 @default.
- W2808297243 cites W2015119856 @default.
- W2808297243 cites W2020669856 @default.
- W2808297243 cites W2026491405 @default.
- W2808297243 cites W2027933379 @default.
- W2808297243 cites W2036657053 @default.
- W2808297243 cites W2047195096 @default.
- W2808297243 cites W2048022791 @default.
- W2808297243 cites W2054537975 @default.
- W2808297243 cites W2062073185 @default.
- W2808297243 cites W2062080461 @default.
- W2808297243 cites W2066020973 @default.
- W2808297243 cites W2081110928 @default.
- W2808297243 cites W2085119196 @default.
- W2808297243 cites W2095526875 @default.
- W2808297243 cites W2101307089 @default.
- W2808297243 cites W2127007298 @default.
- W2808297243 cites W2128454097 @default.
- W2808297243 cites W2131240339 @default.
- W2808297243 cites W2140808096 @default.
- W2808297243 cites W2141590023 @default.
- W2808297243 cites W2142240162 @default.
- W2808297243 cites W2145149977 @default.
- W2808297243 cites W2212838866 @default.
- W2808297243 cites W2316565238 @default.
- W2808297243 cites W2494588723 @default.
- W2808297243 cites W2568598909 @default.
- W2808297243 cites W2616688059 @default.
- W2808297243 cites W2754709197 @default.
- W2808297243 cites W2766295901 @default.
- W2808297243 cites W2801734535 @default.
- W2808297243 cites W4317528178 @default.
- W2808297243 cites W6793813 @default.
- W2808297243 doi "https://doi.org/10.1007/s11427-017-9304-8" @default.
- W2808297243 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29909473" @default.
- W2808297243 hasPublicationYear "2018" @default.
- W2808297243 type Work @default.
- W2808297243 sameAs 2808297243 @default.
- W2808297243 citedByCount "11" @default.
- W2808297243 countsByYear W28082972432019 @default.
- W2808297243 countsByYear W28082972432020 @default.
- W2808297243 countsByYear W28082972432021 @default.
- W2808297243 countsByYear W28082972432022 @default.
- W2808297243 countsByYear W28082972432023 @default.
- W2808297243 crossrefType "journal-article" @default.
- W2808297243 hasAuthorship W2808297243A5000432967 @default.
- W2808297243 hasAuthorship W2808297243A5018592150 @default.
- W2808297243 hasAuthorship W2808297243A5058910088 @default.
- W2808297243 hasAuthorship W2808297243A5087785658 @default.
- W2808297243 hasBestOaLocation W28082972431 @default.
- W2808297243 hasConcept C101762097 @default.
- W2808297243 hasConcept C104317684 @default.
- W2808297243 hasConcept C143065580 @default.
- W2808297243 hasConcept C150194340 @default.
- W2808297243 hasConcept C181199279 @default.
- W2808297243 hasConcept C2776923806 @default.
- W2808297243 hasConcept C2778906449 @default.
- W2808297243 hasConcept C2781204770 @default.
- W2808297243 hasConcept C55493867 @default.
- W2808297243 hasConcept C76935637 @default.
- W2808297243 hasConcept C86803240 @default.
- W2808297243 hasConcept C89423630 @default.
- W2808297243 hasConceptScore W2808297243C101762097 @default.
- W2808297243 hasConceptScore W2808297243C104317684 @default.
- W2808297243 hasConceptScore W2808297243C143065580 @default.
- W2808297243 hasConceptScore W2808297243C150194340 @default.
- W2808297243 hasConceptScore W2808297243C181199279 @default.
- W2808297243 hasConceptScore W2808297243C2776923806 @default.
- W2808297243 hasConceptScore W2808297243C2778906449 @default.
- W2808297243 hasConceptScore W2808297243C2781204770 @default.
- W2808297243 hasConceptScore W2808297243C55493867 @default.
- W2808297243 hasConceptScore W2808297243C76935637 @default.
- W2808297243 hasConceptScore W2808297243C86803240 @default.
- W2808297243 hasConceptScore W2808297243C89423630 @default.
- W2808297243 hasIssue "1" @default.
- W2808297243 hasLocation W28082972431 @default.