FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2808368410> ?p ?o ?g. }

• W2808368410 abstract "Abstract PCR-based techniques are widely used to identify disease causing bacterial and viral pathogens, especially in point-of-care or near-patient clinical settings that require rapid results and sample-to-answer workflows. However, such techniques often fail to differentiate between closely related species that have highly variable genomes. Here, a homogenous (closed-tube) pathogen identification and classification method is described that combines PCR amplification, array-based amplicon sequence verification, and real-time detection using an inverse fluorescence fluorescence-resonance energy transfer technique. The amplification is designed to satisfy the inclusivity criteria and create ssDNA amplicons, bearing a nonradiating quencher moiety at the 5ʹ-terminus, for all the related species. The array includes fluorescent-labeled probes which preferentially capture the variants of the amplicons and classify them through solid-phase thermal denaturing (melt curve) analysis. Systematic primer and probe design algorithms and empirical validation methods are presented and successfully applied to the challenging example of identification of, and differentiation between, closely related human rhinovirus and human enterovirus strains." @default.
• W2808368410 created "2018-06-21" @default.
• W2808368410 creator A5001878346 @default.
• W2808368410 creator A5009088563 @default.
• W2808368410 creator A5017973768 @default.
• W2808368410 creator A5018893803 @default.
• W2808368410 creator A5032402763 @default.
• W2808368410 creator A5034071991 @default.
• W2808368410 creator A5041641444 @default.
• W2808368410 creator A5042220718 @default.
• W2808368410 creator A5048609317 @default.
• W2808368410 creator A5049004927 @default.
• W2808368410 creator A5052053416 @default.
• W2808368410 creator A5063233419 @default.
• W2808368410 creator A5065462577 @default.
• W2808368410 creator A5073678594 @default.
• W2808368410 creator A5078145976 @default.
• W2808368410 creator A5078258748 @default.
• W2808368410 creator A5082433650 @default.
• W2808368410 creator A5087782201 @default.
• W2808368410 date "2018-01-01" @default.
• W2808368410 modified "2023-09-23" @default.
• W2808368410 title "An array-based melt curve analysis method for the identification and classification of closely related pathogen strains" @default.
• W2808368410 cites W1861083333 @default.
• W2808368410 cites W1963659754 @default.
• W2808368410 cites W1986107076 @default.
• W2808368410 cites W2004803703 @default.
• W2808368410 cites W2021805692 @default.
• W2808368410 cites W2024211260 @default.
• W2808368410 cites W2043534974 @default.
• W2808368410 cites W2045213815 @default.
• W2808368410 cites W2048255163 @default.
• W2808368410 cites W2057000344 @default.
• W2808368410 cites W2057816031 @default.
• W2808368410 cites W2062376685 @default.
• W2808368410 cites W2072556768 @default.
• W2808368410 cites W2074703985 @default.
• W2808368410 cites W2076958395 @default.
• W2808368410 cites W2082571397 @default.
• W2808368410 cites W2097865497 @default.
• W2808368410 cites W2101525386 @default.
• W2808368410 cites W2112823007 @default.
• W2808368410 cites W2113678082 @default.
• W2808368410 cites W2126784042 @default.
• W2808368410 cites W2130794350 @default.
• W2808368410 cites W2132926880 @default.
• W2808368410 cites W2135332195 @default.
• W2808368410 cites W2136309995 @default.
• W2808368410 cites W2149279986 @default.
• W2808368410 cites W2149769193 @default.
• W2808368410 cites W2414721715 @default.
• W2808368410 cites W2460266206 @default.
• W2808368410 cites W2608617863 @default.
• W2808368410 cites W4242729757 @default.
• W2808368410 cites W83049644 @default.
• W2808368410 doi "https://doi.org/10.1093/biomethods/bpy005" @default.
• W2808368410 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6994036" @default.
• W2808368410 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32161799" @default.
• W2808368410 hasPublicationYear "2018" @default.
• W2808368410 type Work @default.
• W2808368410 sameAs 2808368410 @default.
• W2808368410 citedByCount "0" @default.
• W2808368410 crossrefType "journal-article" @default.
• W2808368410 hasAuthorship W2808368410A5001878346 @default.
• W2808368410 hasAuthorship W2808368410A5009088563 @default.
• W2808368410 hasAuthorship W2808368410A5017973768 @default.
• W2808368410 hasAuthorship W2808368410A5018893803 @default.
• W2808368410 hasAuthorship W2808368410A5032402763 @default.
• W2808368410 hasAuthorship W2808368410A5034071991 @default.
• W2808368410 hasAuthorship W2808368410A5041641444 @default.
• W2808368410 hasAuthorship W2808368410A5042220718 @default.
• W2808368410 hasAuthorship W2808368410A5048609317 @default.
• W2808368410 hasAuthorship W2808368410A5049004927 @default.
• W2808368410 hasAuthorship W2808368410A5052053416 @default.
• W2808368410 hasAuthorship W2808368410A5063233419 @default.
• W2808368410 hasAuthorship W2808368410A5065462577 @default.
• W2808368410 hasAuthorship W2808368410A5073678594 @default.
• W2808368410 hasAuthorship W2808368410A5078145976 @default.
• W2808368410 hasAuthorship W2808368410A5078258748 @default.
• W2808368410 hasAuthorship W2808368410A5082433650 @default.
• W2808368410 hasAuthorship W2808368410A5087782201 @default.
• W2808368410 hasBestOaLocation W28083684101 @default.
• W2808368410 hasConcept C104317684 @default.
• W2808368410 hasConcept C116834253 @default.
• W2808368410 hasConcept C121332964 @default.
• W2808368410 hasConcept C178790620 @default.
• W2808368410 hasConcept C185592680 @default.
• W2808368410 hasConcept C2777563447 @default.
• W2808368410 hasConcept C49105822 @default.
• W2808368410 hasConcept C54355233 @default.
• W2808368410 hasConcept C59822182 @default.
• W2808368410 hasConcept C62520636 @default.
• W2808368410 hasConcept C70721500 @default.
• W2808368410 hasConcept C8185291 @default.
• W2808368410 hasConcept C86803240 @default.
• W2808368410 hasConcept C91881484 @default.
• W2808368410 hasConcept C96305047 @default.
• W2808368410 hasConceptScore W2808368410C104317684 @default.
• W2808368410 hasConceptScore W2808368410C116834253 @default.
• W2808368410 hasConceptScore W2808368410C121332964 @default.

• next