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• W2808415072 abstract "Lactose malabsorption is normally evaluated by measuring exhaled H2 produced by intestinal flora, from unabsorbed lactose. However, differing microbiome composition can lead to the production of CH4 instead of H2; hence, some authors challenge the H2 method sensitivity and favor the evaluation of both intestinal gases.To compare different approaches to usage of a lactose breath test for lactose malabsorption diagnosis, after medical evaluation of gastrointestinal symptoms.In a retrospective observational study, we compared the 2 approaches in a population of 282 subjects in Northern Italy. Following oral lactose administration, exhaled samples were harvested every 30 minutes for 4 hours and prepared for H2 and CH4 analysis. Basal gas levels were subtracted from H2 and CH4 ppm and values at 4 hours and peaks were considered for analysis.Applying the standard methodology, which takes separately into consideration H2 and CH4 produced in the intestinal lumen, the results indicated that 11.7% of the patients were diagnosed positive for hypolactasia, differently from what was expected. Conversely, taking into consideration the sum of H2 and CH4, the percentage increased to 62.8%, closer to the expected one. No significant differences were found when comparing the 2 groups for age, gender, or symptoms. The sizable difference between the 2 approaches is likely linked to gut microbiome variability, and consequently the different production of the 2 gases, in the population.The threshold normally used for lactose breath test should be reconsidered and changed, merging H2 and CH4 stoichiometric values to increase sensitivity." @default.
• W2808415072 created "2018-06-21" @default.
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• W2808415072 date "2018-06-01" @default.
• W2808415072 modified "2023-10-16" @default.
• W2808415072 title "Retrospective analysis of a lactose breath test in a gastrointestinal symptomatic population of Northeast Italy: use of (H₂+2CH₄) versus H₂ threshold" @default.
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• W2808415072 doi "https://doi.org/10.2147/ceg.s163962" @default.
• W2808415072 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6011881" @default.
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