FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2808566909> ?p ?o ?g. }

• W2808566909 abstract "To investigate the effect of stimulation of human bronchial epithelial cells (HBECs) by arterial traffic ambient PM2.5 (TAPM2.5) and wood smoke PM2.5 (WSPM2.5) on the expression of long non-coding RNAs (lncRNAs) in order to find new therapeutic targets for treatment of chronic obstructive pulmonary disease (COPD). HBECs were exposed to TAPM2.5 and WSPM2.5 at a series of concentrations. The microarray analysis was used to detect the lncRNA and mRNA expression profiles. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis and gene ontology (GO) enrichment were conducted to analyze the differentially expressed lncRNAs and mRNAs. Quantitative real-time PCR (qRT-PCR) was performed to confirm the differential expression of lncRNAs. Western blot was performed to study the expression of autophagy and apoptosis-associated proteins. Flow cytometry was used to detect the apoptotic cells. The results indicated that fine particulate matter (PM2.5)-induced cell damage of HBECs occurred in a dose-dependent manner. The microarray analysis indicated that treatment with TAPM2.5 and WSPM2.5 led to the alteration of lncRNA and mRNA expression profiles. LncRNA maternally expressed gene 3 (MEG3) was significantly up-regulated in HBECs after PM2.5 treatment. The results of Western blot showed that PM2.5 induced cell apoptosis and autophagy by up-regulating apoptosis-associated gene, caspase-3, and down-regulating autophagy-associated markers, Bcl-2 and LC3 expression. In addition, we demonstrated that TAPM2.5 and WSPM2.5 accelerated apoptosis of human bronchial (HBE) cells, silencing of MEG3 suppressed apoptosis and autophagy of HBE cells. These findings suggested that the lncRNA MEG3 mediates PM2.5-induced cell apoptosis and autophagy, and probably through regulating the expression of p53." @default.
• W2808566909 created "2018-06-21" @default.
• W2808566909 creator A5002244546 @default.
• W2808566909 creator A5002882614 @default.
• W2808566909 creator A5002941281 @default.
• W2808566909 creator A5019216175 @default.
• W2808566909 creator A5038804418 @default.
• W2808566909 creator A5055285964 @default.
• W2808566909 creator A5056630066 @default.
• W2808566909 creator A5071081238 @default.
• W2808566909 creator A5075196242 @default.
• W2808566909 creator A5079156087 @default.
• W2808566909 creator A5080235935 @default.
• W2808566909 date "2018-09-12" @default.
• W2808566909 modified "2023-10-05" @default.
• W2808566909 title "Identification of abnormally expressed lncRNAs induced by PM2.5 in human bronchial epithelial cells" @default.
• W2808566909 cites W1013088823 @default.
• W2808566909 cites W1528865568 @default.
• W2808566909 cites W1571858136 @default.
• W2808566909 cites W1830298985 @default.
• W2808566909 cites W1864729394 @default.
• W2808566909 cites W1968711495 @default.
• W2808566909 cites W1971120393 @default.
• W2808566909 cites W1972009654 @default.
• W2808566909 cites W1979529929 @default.
• W2808566909 cites W1979783084 @default.
• W2808566909 cites W1980402007 @default.
• W2808566909 cites W1981790437 @default.
• W2808566909 cites W1984972028 @default.
• W2808566909 cites W1991885150 @default.
• W2808566909 cites W1999114313 @default.
• W2808566909 cites W1999746344 @default.
• W2808566909 cites W2000833392 @default.
• W2808566909 cites W2003544524 @default.
• W2808566909 cites W2005165818 @default.
• W2808566909 cites W2012738194 @default.
• W2808566909 cites W2021129711 @default.
• W2808566909 cites W2025126989 @default.
• W2808566909 cites W2031880275 @default.
• W2808566909 cites W2036057895 @default.
• W2808566909 cites W2039816251 @default.
• W2808566909 cites W2053911859 @default.
• W2808566909 cites W2057094700 @default.
• W2808566909 cites W2064587667 @default.
• W2808566909 cites W2065911962 @default.
• W2808566909 cites W2076810056 @default.
• W2808566909 cites W2082540550 @default.
• W2808566909 cites W2083033504 @default.
• W2808566909 cites W2089417149 @default.
• W2808566909 cites W2093007278 @default.
• W2808566909 cites W2100982253 @default.
• W2808566909 cites W2102146512 @default.
• W2808566909 cites W2103639573 @default.
• W2808566909 cites W2104786214 @default.
• W2808566909 cites W2104798171 @default.
• W2808566909 cites W2108238605 @default.
• W2808566909 cites W2113558571 @default.
• W2808566909 cites W2113629891 @default.
• W2808566909 cites W2115201309 @default.
• W2808566909 cites W2116168949 @default.
• W2808566909 cites W2116236561 @default.
• W2808566909 cites W2120435825 @default.
• W2808566909 cites W2126504590 @default.
• W2808566909 cites W2128643205 @default.
• W2808566909 cites W2130388134 @default.
• W2808566909 cites W2136625809 @default.
• W2808566909 cites W2148629421 @default.
• W2808566909 cites W2151456458 @default.
• W2808566909 cites W2161483818 @default.
• W2808566909 cites W2174891444 @default.
• W2808566909 cites W2204583106 @default.
• W2808566909 cites W2212385672 @default.
• W2808566909 cites W2231496468 @default.
• W2808566909 cites W2268917047 @default.
• W2808566909 cites W2282578737 @default.
• W2808566909 cites W2313170633 @default.
• W2808566909 cites W2321127785 @default.
• W2808566909 cites W2337910658 @default.
• W2808566909 cites W2345769277 @default.
• W2808566909 cites W2384762556 @default.
• W2808566909 cites W2403101766 @default.
• W2808566909 cites W2409650768 @default.
• W2808566909 cites W2471252803 @default.
• W2808566909 cites W2474160866 @default.
• W2808566909 cites W2511592073 @default.
• W2808566909 cites W2552206015 @default.
• W2808566909 cites W2555885116 @default.
• W2808566909 cites W2590972178 @default.
• W2808566909 cites W2592919648 @default.
• W2808566909 cites W2602344822 @default.
• W2808566909 cites W2610236305 @default.
• W2808566909 cites W2753546472 @default.
• W2808566909 cites W2981709490 @default.
• W2808566909 cites W2397374099 @default.
• W2808566909 doi "https://doi.org/10.1042/bsr20171577" @default.
• W2808566909 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6131355" @default.
• W2808566909 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29899163" @default.
• W2808566909 hasPublicationYear "2018" @default.
• W2808566909 type Work @default.
• W2808566909 sameAs 2808566909 @default.

• next