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• W2808894503 abstract "Deinagkistrodon acutus, Trimeresurus stejnegeri, Protobothrops mucrosquamatus, Daboia russelii siamensis, Bungarus multicinctus and Naja atra are the six medically important venomous snake species in Taiwan. In this study, we characterized and compared their venom protein profiles using proteomic approaches. The major snake venom proteins were identified by GeLC-MS/MS and the total venom proteome was characterized by in-solution digestion coupled with LC-MS/MS. A total of 27–52 proteins, categorized into 23 protein families, were identified in each snake's venom. The major venom components found in Viperidae species (D. acutus, T. stejnegeri, P. mucrosquamatus and D. russelii) were C-type lectin, snake venom serine proteinase, venom metalloproteinase and phospholipase A2, whereas three-finger toxin and phospholipase A2 were the major components detected in the venom of Elapidae snakes (B. multicinctus and N. atra). This study also provided the first demonstration of some low-abundance proteins in these six snake venoms, including 5′-nucleotidase, glutaminyl-peptide cyclotransferase and phosphodiesterase, among others. Furthermore, we found that cobra venom factor (CVF) is a cobra-specific protein. We produced anti-peptide antibodies against CVF and used it to develop a highly sensitive SISCAPA-MRM assay for quantifying CVF. The limit of detection and lower limit of quantification were 3.2 and 9.6 attomoles, respectively. This assay was used to precisely quantify CVF in 1 μg crude venom proteins from three Naja species and king cobra. The amount of CVF varied from 0.9 to 54.36 femtomoles (equivalent to 0.16–10.03 mg/g of venom protein). There are six medically significant venomous snakes in Taiwan. The venoms of the four Viperidae species (Deinagkistrodon acutus, Trimeresurus stejnegeri, Protobothrops mucrosquamatus and Daboia russelii siamensis) cause local tissue swelling; this symptom is also seen in N. atra envenomation in humans, potentially complicating the differential diagnosis of envenomation by N. atra and Viperidae species. Thus, characterization of the venom proteomes of the six Taiwanese snakes, including the relative abundance of the major components and species-specific protein(s) in each venom type, could be useful for future venom research, including the development of new assay(s) for detecting snake species-specific venom protein(s) and new type(s) of antivenom." @default.
• W2808894503 created "2018-06-29" @default.
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• W2808894503 date "2018-09-01" @default.
• W2808894503 modified "2023-10-17" @default.
• W2808894503 title "Proteomic characterization of six Taiwanese snake venoms: Identification of species-specific proteins and development of a SISCAPA-MRM assay for cobra venom factors" @default.
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• W2808894503 cites W1892810170 @default.
• W2808894503 cites W1964233256 @default.
• W2808894503 cites W1965102118 @default.
• W2808894503 cites W1966556984 @default.
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• W2808894503 cites W1980428805 @default.
• W2808894503 cites W1985237517 @default.
• W2808894503 cites W1986643911 @default.
• W2808894503 cites W1995640513 @default.
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• W2808894503 cites W2021640998 @default.
• W2808894503 cites W2023897662 @default.
• W2808894503 cites W2029622823 @default.
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• W2808894503 cites W2033408480 @default.
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• W2808894503 cites W2041547469 @default.
• W2808894503 cites W2043276694 @default.
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• W2808894503 doi "https://doi.org/10.1016/j.jprot.2018.06.003" @default.
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