FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2809050290> ?p ?o ?g. }

• W2809050290 endingPage "e201800088" @default.
• W2809050290 startingPage "e201800088" @default.
• W2809050290 abstract "Proteins interact with nucleic acids to regulate cellular functions. The study of these regulatory interactions is often hampered by the limited efficiency of current protocols to isolate the relevant nucleic acid–protein complexes. In this report, we describe a rapid and simple procedure to highly enrich cross-linked nucleic acid–bound proteins, referred to as “2C” for “complex capture.” This method is based on the observation that silica matrix–based columns used for nucleic acid purification also effectively retain UV cross-linked nucleic acid–protein complexes. As a proof of principle, 2C was used to isolate RNA-bound proteins from yeast and mammalian Huh7 cells. The 2C method makes RNA labelling redundant, and specific RNA–protein interactions can be observed and validated by Western blotting. RNA–protein complexes isolated by 2C can subsequently be immunoprecipitated, showing that 2C is in principle compatible with sensitive downstream applications. We suggest that 2C can dramatically simplify the study of nucleic acid–protein interactions and benefit researchers in the fields of DNA and RNA biology." @default.
• W2809050290 created "2018-06-29" @default.
• W2809050290 creator A5000270755 @default.
• W2809050290 creator A5050096213 @default.
• W2809050290 creator A5061953363 @default.
• W2809050290 date "2018-06-01" @default.
• W2809050290 modified "2023-10-11" @default.
• W2809050290 title "Silica-based solid-phase extraction of cross-linked nucleic acid–bound proteins" @default.
• W2809050290 cites W1010098085 @default.
• W2809050290 cites W1545744330 @default.
• W2809050290 cites W1696199194 @default.
• W2809050290 cites W1942208622 @default.
• W2809050290 cites W1972464137 @default.
• W2809050290 cites W1974848291 @default.
• W2809050290 cites W1986911150 @default.
• W2809050290 cites W2025075195 @default.
• W2809050290 cites W2033253992 @default.
• W2809050290 cites W2040356728 @default.
• W2809050290 cites W2041440237 @default.
• W2809050290 cites W2052668460 @default.
• W2809050290 cites W2060333964 @default.
• W2809050290 cites W2116635614 @default.
• W2809050290 cites W2126194787 @default.
• W2809050290 cites W2134193463 @default.
• W2809050290 cites W2136794803 @default.
• W2809050290 cites W2166229810 @default.
• W2809050290 cites W2170265331 @default.
• W2809050290 cites W2171111703 @default.
• W2809050290 cites W2182726182 @default.
• W2809050290 cites W2307041907 @default.
• W2809050290 cites W2480300184 @default.
• W2809050290 cites W2523076424 @default.
• W2809050290 cites W2539232660 @default.
• W2809050290 cites W2568577966 @default.
• W2809050290 cites W2592838654 @default.
• W2809050290 cites W2632422275 @default.
• W2809050290 cites W2760202013 @default.
• W2809050290 cites W2765126569 @default.
• W2809050290 cites W2766052208 @default.
• W2809050290 cites W2785329615 @default.
• W2809050290 cites W2786634643 @default.
• W2809050290 cites W278790693 @default.
• W2809050290 cites W2797140845 @default.
• W2809050290 cites W4294216491 @default.
• W2809050290 cites W4790534 @default.
• W2809050290 doi "https://doi.org/10.26508/lsa.201800088" @default.
• W2809050290 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6054301" @default.
• W2809050290 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30035255" @default.
• W2809050290 hasPublicationYear "2018" @default.
• W2809050290 type Work @default.
• W2809050290 sameAs 2809050290 @default.
• W2809050290 citedByCount "44" @default.
• W2809050290 countsByYear W28090502902018 @default.
• W2809050290 countsByYear W28090502902019 @default.
• W2809050290 countsByYear W28090502902020 @default.
• W2809050290 countsByYear W28090502902021 @default.
• W2809050290 countsByYear W28090502902022 @default.
• W2809050290 countsByYear W28090502902023 @default.
• W2809050290 crossrefType "journal-article" @default.
• W2809050290 hasAuthorship W2809050290A5000270755 @default.
• W2809050290 hasAuthorship W2809050290A5050096213 @default.
• W2809050290 hasAuthorship W2809050290A5061953363 @default.
• W2809050290 hasBestOaLocation W28090502901 @default.
• W2809050290 hasConcept C104317684 @default.
• W2809050290 hasConcept C126142528 @default.
• W2809050290 hasConcept C153289436 @default.
• W2809050290 hasConcept C185592680 @default.
• W2809050290 hasConcept C24107716 @default.
• W2809050290 hasConcept C25862648 @default.
• W2809050290 hasConcept C41282012 @default.
• W2809050290 hasConcept C54871578 @default.
• W2809050290 hasConcept C552990157 @default.
• W2809050290 hasConcept C55493867 @default.
• W2809050290 hasConcept C67705224 @default.
• W2809050290 hasConcept C70721500 @default.
• W2809050290 hasConcept C86803240 @default.
• W2809050290 hasConceptScore W2809050290C104317684 @default.
• W2809050290 hasConceptScore W2809050290C126142528 @default.
• W2809050290 hasConceptScore W2809050290C153289436 @default.
• W2809050290 hasConceptScore W2809050290C185592680 @default.
• W2809050290 hasConceptScore W2809050290C24107716 @default.
• W2809050290 hasConceptScore W2809050290C25862648 @default.
• W2809050290 hasConceptScore W2809050290C41282012 @default.
• W2809050290 hasConceptScore W2809050290C54871578 @default.
• W2809050290 hasConceptScore W2809050290C552990157 @default.
• W2809050290 hasConceptScore W2809050290C55493867 @default.
• W2809050290 hasConceptScore W2809050290C67705224 @default.
• W2809050290 hasConceptScore W2809050290C70721500 @default.
• W2809050290 hasConceptScore W2809050290C86803240 @default.
• W2809050290 hasIssue "3" @default.
• W2809050290 hasLocation W28090502901 @default.
• W2809050290 hasLocation W28090502902 @default.
• W2809050290 hasLocation W28090502903 @default.
• W2809050290 hasLocation W28090502904 @default.
• W2809050290 hasLocation W28090502905 @default.
• W2809050290 hasOpenAccess W2809050290 @default.
• W2809050290 hasPrimaryLocation W28090502901 @default.
• W2809050290 hasRelatedWork W1508798449 @default.

• next