FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2809145153> ?p ?o ?g. }

• W2809145153 endingPage "826" @default.
• W2809145153 startingPage "815" @default.
• W2809145153 abstract "Single-domain antibodies (sdAbs), the autonomous variable domains of heavy chain-only antibodies produced naturally by camelid ungulates and cartilaginous fishes, have evolved to bind antigen using only three complementarity-determining region (CDR) loops rather than the six present in conventional VH:VL antibodies. It has been suggested, based on limited evidence, that sdAbs may adopt paratope structures that predispose them to preferential recognition of recessed protein epitopes, but poor or non-recognition of protuberant epitopes and small molecules. Here, we comprehensively surveyed the evidence in support of this hypothesis. We found some support for a global structural difference in the paratope shapes of sdAbs compared with those of conventional antibodies: sdAb paratopes have smaller molecular surface areas and diameters, more commonly have non-canonical CDR1 and CDR2 structures, and have elongated CDR3 length distributions, but have similar amino acid compositions and are no more extended (interatomic distance measured from CDR base to tip) than conventional antibody paratopes. Comparison of X-ray crystal structures of sdAbs and conventional antibodies in complex with cognate antigens showed that sdAbs and conventional antibodies bury similar solvent-exposed surface areas on proteins and form similar types of non-covalent interactions, although these are more concentrated in the compact sdAb paratope. Thus, sdAbs likely have privileged access to distinct antigenic regions on proteins, but only owing to their small molecular size and not to general differences in molecular recognition mechanism. The evidence surrounding the purported inability of sdAbs to bind small molecules was less clear. The available data provide a structural framework for understanding the evolutionary emergence and function of autonomous heavy chain-only antibodies." @default.
• W2809145153 created "2018-06-29" @default.
• W2809145153 creator A5016428141 @default.
• W2809145153 creator A5077226777 @default.
• W2809145153 date "2018-08-15" @default.
• W2809145153 modified "2023-10-13" @default.
• W2809145153 title "Antigen recognition by single-domain antibodies: structural latitudes and constraints" @default.
• W2809145153 cites W1487255561 @default.
• W2809145153 cites W1532849119 @default.
• W2809145153 cites W1758955113 @default.
• W2809145153 cites W1902656581 @default.
• W2809145153 cites W1918796765 @default.
• W2809145153 cites W1964538987 @default.
• W2809145153 cites W1973032325 @default.
• W2809145153 cites W1975758824 @default.
• W2809145153 cites W1978217631 @default.
• W2809145153 cites W1980758834 @default.
• W2809145153 cites W1984227078 @default.
• W2809145153 cites W1985154010 @default.
• W2809145153 cites W1985534966 @default.
• W2809145153 cites W1985731056 @default.
• W2809145153 cites W1988176721 @default.
• W2809145153 cites W1988778953 @default.
• W2809145153 cites W1989201900 @default.
• W2809145153 cites W1989275845 @default.
• W2809145153 cites W1989672802 @default.
• W2809145153 cites W1992021982 @default.
• W2809145153 cites W1992032455 @default.
• W2809145153 cites W1993575340 @default.
• W2809145153 cites W1993791286 @default.
• W2809145153 cites W1996538960 @default.
• W2809145153 cites W1998292458 @default.
• W2809145153 cites W1999879329 @default.
• W2809145153 cites W2004682643 @default.
• W2809145153 cites W2009843473 @default.
• W2809145153 cites W2014327275 @default.
• W2809145153 cites W2014604536 @default.
• W2809145153 cites W2019486739 @default.
• W2809145153 cites W2020601776 @default.
• W2809145153 cites W2020972820 @default.
• W2809145153 cites W2021256658 @default.
• W2809145153 cites W2021643610 @default.
• W2809145153 cites W2028671938 @default.
• W2809145153 cites W2028868321 @default.
• W2809145153 cites W2031132894 @default.
• W2809145153 cites W2031989831 @default.
• W2809145153 cites W2033079214 @default.
• W2809145153 cites W2034821602 @default.
• W2809145153 cites W2036745443 @default.
• W2809145153 cites W2037042031 @default.
• W2809145153 cites W2037302035 @default.
• W2809145153 cites W2038652838 @default.
• W2809145153 cites W2039757405 @default.
• W2809145153 cites W2041875415 @default.
• W2809145153 cites W2042401358 @default.
• W2809145153 cites W2043051805 @default.
• W2809145153 cites W2043656421 @default.
• W2809145153 cites W2051994079 @default.
• W2809145153 cites W2053422042 @default.
• W2809145153 cites W2055378587 @default.
• W2809145153 cites W2062653170 @default.
• W2809145153 cites W2063926965 @default.
• W2809145153 cites W2073926543 @default.
• W2809145153 cites W2079277731 @default.
• W2809145153 cites W2080799466 @default.
• W2809145153 cites W2081991152 @default.
• W2809145153 cites W2083669011 @default.
• W2809145153 cites W2089788910 @default.
• W2809145153 cites W2090383712 @default.
• W2809145153 cites W2090916171 @default.
• W2809145153 cites W2094656087 @default.
• W2809145153 cites W2095103144 @default.
• W2809145153 cites W2095553811 @default.
• W2809145153 cites W2098017245 @default.
• W2809145153 cites W2099090318 @default.
• W2809145153 cites W2101398436 @default.
• W2809145153 cites W2104115178 @default.
• W2809145153 cites W2105360462 @default.
• W2809145153 cites W2106355732 @default.
• W2809145153 cites W2108162986 @default.
• W2809145153 cites W2110036226 @default.
• W2809145153 cites W2112168752 @default.
• W2809145153 cites W2112782031 @default.
• W2809145153 cites W2113155348 @default.
• W2809145153 cites W2114287433 @default.
• W2809145153 cites W2115912680 @default.
• W2809145153 cites W2118375897 @default.
• W2809145153 cites W2124759299 @default.
• W2809145153 cites W2124967403 @default.
• W2809145153 cites W2128297456 @default.
• W2809145153 cites W2130575889 @default.
• W2809145153 cites W2134599979 @default.
• W2809145153 cites W2140060739 @default.
• W2809145153 cites W2150804466 @default.
• W2809145153 cites W2151197219 @default.
• W2809145153 cites W2159047076 @default.
• W2809145153 cites W2160652302 @default.
• W2809145153 cites W2161709523 @default.

• next