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- W2809417427 abstract "In this study, we presented a new coating regulation of silica by the reverse microemulsion method to obtain biocompatible high quality silica-encapsulated quantum dot (QD). The hydrophobic CdSe/ZnS QDs were coated with silica successfully and demonstrated good photostability and thermal stability and high fluorescent intensity. The corresponding silica-encapsulated QDs-antibody probes were used to detect C-reactive protein (CRP) with quantum dot-labeled immunosorbent assay (QLISA). Only micro dosage of probes was required due to good antibody-antigen combination in optimized buffer environment. The assay results are comparable to commercial assay kits with a wide analytical range of 1-1000 ng/mL, a low limit of detection of 0.76 ng/mL, and good recovery rates of 87.31–105.48%. The convenience and cost effective aspects of this silica-encapsulated QDs immunosorbent assay motivate future development of in vitro diagnostic kits." @default.
- W2809417427 created "2018-06-29" @default.
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- W2809417427 date "2018-09-01" @default.
- W2809417427 modified "2023-10-13" @default.
- W2809417427 title "Silica-encapsulated quantum dots for highly efficient and stable fluorescence immunoassay of C-reactive protein" @default.
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- W2809417427 doi "https://doi.org/10.1016/j.bej.2018.06.016" @default.
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