FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2809438503> ?p ?o ?g. }

• W2809438503 endingPage "332" @default.
• W2809438503 startingPage "319" @default.
• W2809438503 abstract "Biomaterials can interact with cells directly, that is, by direct contact of the cells with the material surface, or indirectly, through soluble species that can be released to or uptaken from the surrounding fluids. However, it is difficult to characterise the relevance of this fluid-mediated interaction separately from the topography and composition of the substrate, because they are coupled variables. These fluid-mediated interactions are amplified in the case of highly reactive calcium phosphates (CaPs) such as biomimetic calcium deficient hydroxyapatite (CDHA), particularly in static in vitro cultures. The present work proposes a strategy to decouple the effect of ion exchange from topographical features by adjusting the volume ratio between the cell culture medium and biomaterial (VCM/VB). Increasing this ratio allowed mitigating the drastic ionic exchanges associated to the compositional changes experienced by the material exposed to the cell culture medium. This strategy was validated using rat mesenchymal stem cells (rMSCs) cultured on CDHA and beta-tricalcium phosphate (β-TCP) discs using different VCM/VB ratios. Whereas in the case of β-TCP the cell response was not affected by this ratio, a significant effect on cell adhesion and proliferation was found for the more reactive CDHA. The ionic exchange, produced by CDHA at low VCM/VB, altered cell adhesion due to the reduced number of focal adhesions, caused cell shrinkage and further rMCSs apoptosis. This was mitigated when using a high VCM/VB, which attenuated the changes of calcium and phosphate concentrations in the cell culture medium, resulting in rMSCs spreading and a viability over time. Moreover, rMSCs showed an earlier expression of osteogenic genes on CDHA compared to sintered β-TCP when extracellular calcium fluctuations were reduced. Fluid mediated interactions play a significant role in the bioactivity of calcium phosphates. Ionic exchange is amplified in the case of biomimetic hydroxyapatite, which makes the in vitro characterisation of cell-material interactions especially challenging. The present work proposes a novel and simple strategy to explore the mechanisms of interaction of biomimetic and sintered calcium phosphates with mesenchymal stem cells. The effects of topography and ion exchange are analysed separately by modifying the volume ratio between cell culture medium and biomaterial. High ionic fluctuations interfered in the maturation of focal adhesions, hampering cell adhesion and leading to increased apoptosis and reduced proliferation rate." @default.
• W2809438503 created "2018-06-29" @default.
• W2809438503 creator A5020563927 @default.
• W2809438503 creator A5027548306 @default.
• W2809438503 creator A5037293169 @default.
• W2809438503 creator A5053954520 @default.
• W2809438503 creator A5058778087 @default.
• W2809438503 creator A5083229558 @default.
• W2809438503 date "2018-08-01" @default.
• W2809438503 modified "2023-10-18" @default.
• W2809438503 title "In vitro response of mesenchymal stem cells to biomimetic hydroxyapatite substrates: A new strategy to assess the effect of ion exchange" @default.
• W2809438503 cites W1531447386 @default.
• W2809438503 cites W1599277155 @default.
• W2809438503 cites W1607531254 @default.
• W2809438503 cites W1966935284 @default.
• W2809438503 cites W1976881265 @default.
• W2809438503 cites W1979552683 @default.
• W2809438503 cites W1985648725 @default.
• W2809438503 cites W2001457449 @default.
• W2809438503 cites W2003852585 @default.
• W2809438503 cites W2005983541 @default.
• W2809438503 cites W2011586509 @default.
• W2809438503 cites W2022213375 @default.
• W2809438503 cites W2027461314 @default.
• W2809438503 cites W2032659134 @default.
• W2809438503 cites W2044070730 @default.
• W2809438503 cites W2048379336 @default.
• W2809438503 cites W2050362176 @default.
• W2809438503 cites W2055418337 @default.
• W2809438503 cites W2061621619 @default.
• W2809438503 cites W2065751523 @default.
• W2809438503 cites W2077230011 @default.
• W2809438503 cites W2078494459 @default.
• W2809438503 cites W2080443939 @default.
• W2809438503 cites W2082027561 @default.
• W2809438503 cites W2085162903 @default.
• W2809438503 cites W2086831062 @default.
• W2809438503 cites W2087625365 @default.
• W2809438503 cites W2107438214 @default.
• W2809438503 cites W2108244474 @default.
• W2809438503 cites W2112440550 @default.
• W2809438503 cites W2117223537 @default.
• W2809438503 cites W2119763108 @default.
• W2809438503 cites W2125693919 @default.
• W2809438503 cites W2128777443 @default.
• W2809438503 cites W2147169897 @default.
• W2809438503 cites W2155507447 @default.
• W2809438503 cites W2158005183 @default.
• W2809438503 cites W2159167977 @default.
• W2809438503 cites W2169727595 @default.
• W2809438503 cites W2171179595 @default.
• W2809438503 cites W2244726815 @default.
• W2809438503 cites W2269730577 @default.
• W2809438503 cites W2289952993 @default.
• W2809438503 cites W2334165213 @default.
• W2809438503 cites W2578305725 @default.
• W2809438503 cites W2620146629 @default.
• W2809438503 cites W262912863 @default.
• W2809438503 cites W2737516629 @default.
• W2809438503 cites W2749377108 @default.
• W2809438503 cites W2767237545 @default.
• W2809438503 doi "https://doi.org/10.1016/j.actbio.2018.06.025" @default.
• W2809438503 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29933107" @default.
• W2809438503 hasPublicationYear "2018" @default.
• W2809438503 type Work @default.
• W2809438503 sameAs 2809438503 @default.
• W2809438503 citedByCount "33" @default.
• W2809438503 countsByYear W28094385032018 @default.
• W2809438503 countsByYear W28094385032019 @default.
• W2809438503 countsByYear W28094385032020 @default.
• W2809438503 countsByYear W28094385032021 @default.
• W2809438503 countsByYear W28094385032022 @default.
• W2809438503 crossrefType "journal-article" @default.
• W2809438503 hasAuthorship W2809438503A5020563927 @default.
• W2809438503 hasAuthorship W2809438503A5027548306 @default.
• W2809438503 hasAuthorship W2809438503A5037293169 @default.
• W2809438503 hasAuthorship W2809438503A5053954520 @default.
• W2809438503 hasAuthorship W2809438503A5058778087 @default.
• W2809438503 hasAuthorship W2809438503A5083229558 @default.
• W2809438503 hasBestOaLocation W28094385032 @default.
• W2809438503 hasConcept C12554922 @default.
• W2809438503 hasConcept C136229726 @default.
• W2809438503 hasConcept C1491633281 @default.
• W2809438503 hasConcept C159985019 @default.
• W2809438503 hasConcept C171250308 @default.
• W2809438503 hasConcept C185592680 @default.
• W2809438503 hasConcept C191897082 @default.
• W2809438503 hasConcept C192562407 @default.
• W2809438503 hasConcept C198826908 @default.
• W2809438503 hasConcept C202751555 @default.
• W2809438503 hasConcept C2778414984 @default.
• W2809438503 hasConcept C519063684 @default.
• W2809438503 hasConcept C53227056 @default.
• W2809438503 hasConcept C54355233 @default.
• W2809438503 hasConcept C55493867 @default.
• W2809438503 hasConcept C71924100 @default.
• W2809438503 hasConcept C81885089 @default.
• W2809438503 hasConcept C84416704 @default.

• next