Matches in SemOpenAlex for { <https://semopenalex.org/work/W2809447948> ?p ?o ?g. }
- W2809447948 endingPage "1769" @default.
- W2809447948 startingPage "1745" @default.
- W2809447948 abstract "Malate dehydrogenases (MDHs) convert malate to oxaloacetate using NAD(H) or NADP(H) as a cofactor. Arabidopsis thaliana mutants lacking plastidial NAD-dependent MDH (pdnad-mdh) are embryo-lethal, and constitutive silencing (miR-mdh-1) causes a pale, dwarfed phenotype. The reason for these severe phenotypes is unknown. Here, we rescued the embryo lethality of pdnad-mdh via embryo-specific expression of pdNAD-MDH. Rescued seedlings developed white leaves with aberrant chloroplasts and failed to reproduce. Inducible silencing of pdNAD-MDH at the rosette stage also resulted in white newly emerging leaves. These data suggest that pdNAD-MDH is important for early plastid development, which is consistent with the reductions in major plastidial galactolipid, carotenoid, and protochlorophyllide levels in miR-mdh-1 seedlings. Surprisingly, the targeting of other NAD-dependent MDH isoforms to the plastid did not complement the embryo lethality of pdnad-mdh, while expression of enzymatically inactive pdNAD-MDH did. These complemented plants grew indistinguishably from the wild type. Both active and inactive forms of pdNAD-MDH interact with a heteromeric AAA-ATPase complex at the inner membrane of the chloroplast envelope. Silencing the expression of FtsH12, a key member of this complex, resulted in a phenotype that strongly resembles miR-mdh-1. We propose that pdNAD-MDH is essential for chloroplast development due to its moonlighting role in stabilizing FtsH12, distinct from its enzymatic function." @default.
- W2809447948 created "2018-06-29" @default.
- W2809447948 creator A5015122534 @default.
- W2809447948 creator A5021323127 @default.
- W2809447948 creator A5022573508 @default.
- W2809447948 creator A5024382631 @default.
- W2809447948 creator A5037966423 @default.
- W2809447948 creator A5041713188 @default.
- W2809447948 creator A5049659375 @default.
- W2809447948 creator A5052183230 @default.
- W2809447948 creator A5063533218 @default.
- W2809447948 creator A5067583739 @default.
- W2809447948 creator A5086855287 @default.
- W2809447948 creator A5088369506 @default.
- W2809447948 date "2018-06-22" @default.
- W2809447948 modified "2023-09-30" @default.
- W2809447948 title "Plastidial NAD-Dependent Malate Dehydrogenase: A Moonlighting Protein Involved in Early Chloroplast Development through Its Interaction with an FtsH12-FtsHi Protease Complex" @default.
- W2809447948 cites W1484312809 @default.
- W2809447948 cites W1588094533 @default.
- W2809447948 cites W1595147672 @default.
- W2809447948 cites W1681264417 @default.
- W2809447948 cites W1832711256 @default.
- W2809447948 cites W1917490216 @default.
- W2809447948 cites W1944820832 @default.
- W2809447948 cites W1966992670 @default.
- W2809447948 cites W1969227093 @default.
- W2809447948 cites W1971644029 @default.
- W2809447948 cites W1974372325 @default.
- W2809447948 cites W1976005938 @default.
- W2809447948 cites W1978628425 @default.
- W2809447948 cites W1981548700 @default.
- W2809447948 cites W1981730708 @default.
- W2809447948 cites W1992625839 @default.
- W2809447948 cites W1998708845 @default.
- W2809447948 cites W2000632075 @default.
- W2809447948 cites W2003020761 @default.
- W2809447948 cites W2009326225 @default.
- W2809447948 cites W2014716376 @default.
- W2809447948 cites W2015924572 @default.
- W2809447948 cites W2016950608 @default.
- W2809447948 cites W2018426727 @default.
- W2809447948 cites W2027980348 @default.
- W2809447948 cites W2034944263 @default.
- W2809447948 cites W2035919478 @default.
- W2809447948 cites W2038634422 @default.
- W2809447948 cites W2039278227 @default.
- W2809447948 cites W2042158497 @default.
- W2809447948 cites W2044874780 @default.
- W2809447948 cites W2048450765 @default.
- W2809447948 cites W2050748701 @default.
- W2809447948 cites W2055544854 @default.
- W2809447948 cites W2060687391 @default.
- W2809447948 cites W2061023087 @default.
- W2809447948 cites W2061133426 @default.
- W2809447948 cites W2062942348 @default.
- W2809447948 cites W2065744015 @default.
- W2809447948 cites W2066013441 @default.
- W2809447948 cites W2067535938 @default.
- W2809447948 cites W2073307748 @default.
- W2809447948 cites W2074395745 @default.
- W2809447948 cites W2081204547 @default.
- W2809447948 cites W2082513753 @default.
- W2809447948 cites W2083253012 @default.
- W2809447948 cites W2083922038 @default.
- W2809447948 cites W2086968057 @default.
- W2809447948 cites W2091319046 @default.
- W2809447948 cites W2093931964 @default.
- W2809447948 cites W2098671490 @default.
- W2809447948 cites W2103422734 @default.
- W2809447948 cites W2105202767 @default.
- W2809447948 cites W2107618065 @default.
- W2809447948 cites W2110230346 @default.
- W2809447948 cites W2110435955 @default.
- W2809447948 cites W2113151815 @default.
- W2809447948 cites W2117880160 @default.
- W2809447948 cites W2119399666 @default.
- W2809447948 cites W2121325508 @default.
- W2809447948 cites W2121631499 @default.
- W2809447948 cites W2123360519 @default.
- W2809447948 cites W2123861410 @default.
- W2809447948 cites W2127087804 @default.
- W2809447948 cites W2127967647 @default.
- W2809447948 cites W2128213933 @default.
- W2809447948 cites W2135655483 @default.
- W2809447948 cites W2138112807 @default.
- W2809447948 cites W2145465660 @default.
- W2809447948 cites W2148711203 @default.
- W2809447948 cites W2150984876 @default.
- W2809447948 cites W2152458080 @default.
- W2809447948 cites W2155036894 @default.
- W2809447948 cites W2155867012 @default.
- W2809447948 cites W2158215557 @default.
- W2809447948 cites W2171091522 @default.
- W2809447948 cites W2222440211 @default.
- W2809447948 cites W2289930669 @default.
- W2809447948 cites W2342050947 @default.
- W2809447948 cites W2424241412 @default.
- W2809447948 cites W2511729413 @default.