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• W2809717247 endingPage "12479" @default.
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• W2809717247 abstract "Accurate translation termination by release factors (RFs) is critical for the integrity of cellular proteomes. Premature termination on sense codons, for example, results in truncated proteins, whose accumulation could be detrimental to the cell. Nevertheless, some sense codons are prone to triggering premature termination, but the structural basis for this is unclear. To investigate premature termination, we determined a cryo-EM structure of the Escherichia coli 70S ribosome bound with RF1 in response to a UAU (Tyr) sense codon. The structure reveals that RF1 recognizes a UAU codon similarly to a UAG stop codon, suggesting that sense codons induce premature termination because they structurally mimic a stop codon. Hydrophobic interaction between the nucleobase of U3 (the third position of the UAU codon) and conserved Ile-196 in RF1 is important for misreading the UAU codon. Analyses of RNA binding in ribonucleoprotein complexes or by amino acids reveal that Ile–U packing is a frequent protein–RNA-binding motif with key functional implications. We discuss parallels with eukaryotic translation termination by the release factor eRF1. Accurate translation termination by release factors (RFs) is critical for the integrity of cellular proteomes. Premature termination on sense codons, for example, results in truncated proteins, whose accumulation could be detrimental to the cell. Nevertheless, some sense codons are prone to triggering premature termination, but the structural basis for this is unclear. To investigate premature termination, we determined a cryo-EM structure of the Escherichia coli 70S ribosome bound with RF1 in response to a UAU (Tyr) sense codon. The structure reveals that RF1 recognizes a UAU codon similarly to a UAG stop codon, suggesting that sense codons induce premature termination because they structurally mimic a stop codon. Hydrophobic interaction between the nucleobase of U3 (the third position of the UAU codon) and conserved Ile-196 in RF1 is important for misreading the UAU codon. Analyses of RNA binding in ribonucleoprotein complexes or by amino acids reveal that Ile–U packing is a frequent protein–RNA-binding motif with key functional implications. We discuss parallels with eukaryotic translation termination by the release factor eRF1." @default.
• W2809717247 created "2018-07-10" @default.
• W2809717247 creator A5004824396 @default.
• W2809717247 creator A5007705088 @default.
• W2809717247 creator A5086024663 @default.
• W2809717247 date "2018-08-01" @default.
• W2809717247 modified "2023-10-02" @default.
• W2809717247 title "Mechanism of premature translation termination on a sense codon" @default.
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• W2809717247 doi "https://doi.org/10.1074/jbc.aw118.003232" @default.
• W2809717247 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6093235" @default.
• W2809717247 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29941456" @default.
• W2809717247 hasPublicationYear "2018" @default.
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