FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W280973352> ?p ?o ?g. }

• W280973352 endingPage "261" @default.
• W280973352 startingPage "253" @default.
• W280973352 abstract "Glucose kinase of Streptomyces coelicolor A3(2) is essential for glucose utilisation and is required for carbon catabolite repression (CCR) exerted through glucose and other carbon sources. The protein belongs to the ROK-family, which comprises bacterial sugar kinases and regulators. To better understand glucose kinase function, we have monitored the cellular activity and demonstrated that the choice of carbon sources did not significantly change the synthesis and activity of the enzyme. The DNA sequence of the Streptomyces lividans glucose kinase gene glkA was determined. The predicted gene product of 317 amino acids was found to be identical to S. coelicolor glucose kinase, suggesting a similar role for this protein in both organisms. A procedure was developed to produce pure histidine-tagged glucose kinase with a yield of approximately 10 mg/l culture. The protein was stable for several weeks and was used to raise polyclonal antibodies. Purified glucose kinase was used to explore protein-protein interaction by surface plasmon resonance. The experiments revealed the existence of a binding activity present in S. coelicolor cell extracts. This indicated that glucose kinase may interact with (an)other factor(s), most likely of protein nature. A possible cross-talk with proteins of the phosphotransferase system, which are involved in carbon catabolite repression in other bacteria, was investigated." @default.
• W280973352 created "2016-06-24" @default.
• W280973352 creator A5033578450 @default.
• W280973352 creator A5056003905 @default.
• W280973352 creator A5066000639 @default.
• W280973352 creator A5070347575 @default.
• W280973352 creator A5088736673 @default.
• W280973352 creator A5091052284 @default.
• W280973352 date "2000-01-01" @default.
• W280973352 modified "2023-10-08" @default.
• W280973352 cites W1259650361 @default.
• W280973352 cites W1520001214 @default.
• W280973352 cites W1540276392 @default.
• W280973352 cites W1606418040 @default.
• W280973352 cites W1826496811 @default.
• W280973352 cites W1873666879 @default.
• W280973352 cites W1903153800 @default.
• W280973352 cites W1989734191 @default.
• W280973352 cites W2006417508 @default.
• W280973352 cites W2012940246 @default.
• W280973352 cites W2037564380 @default.
• W280973352 cites W2068040855 @default.
• W280973352 cites W2071955064 @default.
• W280973352 cites W2074776737 @default.
• W280973352 cites W2081308808 @default.
• W280973352 cites W2086432676 @default.
• W280973352 cites W2087903423 @default.
• W280973352 cites W2090307824 @default.
• W280973352 cites W2105005778 @default.
• W280973352 cites W2113756362 @default.
• W280973352 cites W2114742658 @default.
• W280973352 cites W2118817344 @default.
• W280973352 cites W2123569104 @default.
• W280973352 cites W2123722285 @default.
• W280973352 cites W2126403099 @default.
• W280973352 cites W2130394726 @default.
• W280973352 cites W2133270649 @default.
• W280973352 cites W2144634347 @default.
• W280973352 cites W2146220380 @default.
• W280973352 cites W2157151483 @default.
• W280973352 cites W2157902039 @default.
• W280973352 cites W2417143753 @default.
• W280973352 cites W2911960924 @default.
• W280973352 cites W3114686187 @default.
• W280973352 doi "https://doi.org/10.1023/a:1010234916745" @default.
• W280973352 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11386347" @default.
• W280973352 hasPublicationYear "2000" @default.
• W280973352 type Work @default.
• W280973352 sameAs 280973352 @default.
• W280973352 citedByCount "46" @default.
• W280973352 countsByYear W2809733522012 @default.
• W280973352 countsByYear W2809733522013 @default.
• W280973352 countsByYear W2809733522015 @default.
• W280973352 countsByYear W2809733522016 @default.
• W280973352 countsByYear W2809733522017 @default.
• W280973352 countsByYear W2809733522018 @default.
• W280973352 countsByYear W2809733522019 @default.
• W280973352 countsByYear W2809733522020 @default.
• W280973352 countsByYear W2809733522021 @default.
• W280973352 countsByYear W2809733522022 @default.
• W280973352 countsByYear W2809733522023 @default.
• W280973352 crossrefType "journal-article" @default.
• W280973352 hasAuthorship W280973352A5033578450 @default.
• W280973352 hasAuthorship W280973352A5056003905 @default.
• W280973352 hasAuthorship W280973352A5066000639 @default.
• W280973352 hasAuthorship W280973352A5070347575 @default.
• W280973352 hasAuthorship W280973352A5088736673 @default.
• W280973352 hasAuthorship W280973352A5091052284 @default.
• W280973352 hasBestOaLocation W2809733522 @default.
• W280973352 hasConcept C104317684 @default.
• W280973352 hasConcept C143065580 @default.
• W280973352 hasConcept C181199279 @default.
• W280973352 hasConcept C184235292 @default.
• W280973352 hasConcept C2778460671 @default.
• W280973352 hasConcept C2778905257 @default.
• W280973352 hasConcept C2780681812 @default.
• W280973352 hasConcept C55493867 @default.
• W280973352 hasConcept C83941689 @default.
• W280973352 hasConcept C86803240 @default.
• W280973352 hasConcept C97029542 @default.
• W280973352 hasConceptScore W280973352C104317684 @default.
• W280973352 hasConceptScore W280973352C143065580 @default.
• W280973352 hasConceptScore W280973352C181199279 @default.
• W280973352 hasConceptScore W280973352C184235292 @default.
• W280973352 hasConceptScore W280973352C2778460671 @default.
• W280973352 hasConceptScore W280973352C2778905257 @default.
• W280973352 hasConceptScore W280973352C2780681812 @default.
• W280973352 hasConceptScore W280973352C55493867 @default.
• W280973352 hasConceptScore W280973352C83941689 @default.
• W280973352 hasConceptScore W280973352C86803240 @default.
• W280973352 hasConceptScore W280973352C97029542 @default.
• W280973352 hasIssue "3/4" @default.
• W280973352 hasLocation W2809733521 @default.
• W280973352 hasLocation W2809733522 @default.
• W280973352 hasLocation W2809733523 @default.
• W280973352 hasOpenAccess W280973352 @default.
• W280973352 hasPrimaryLocation W2809733521 @default.
• W280973352 hasRelatedWork W1482415806 @default.

• next