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- W2809860508 abstract "Within today's downstream processing of therapeutic proteins, there is an imminent need for alternatives to chromatographic separations, such as aqueous two-phase extraction. Challenges in the development and performance of aqueous two-phase extraction include a number of experiments to define appropriate process windows as well as enable multistage extraction. To reduce experimental effort, a hybrid thermodynamic model is presented in this contribution which allows the extraction system to be optimized with a smaller number of experiments. Moreover, multistage applications of aqueous two-phase extraction are presented to purify enzymes. To purify the investigated enzymes, two different apparatuses were used: namely, a mixer–settler unit and a centrifugal partition chromatograph." @default.
- W2809860508 created "2018-07-10" @default.
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- W2809860508 date "2018-06-19" @default.
- W2809860508 modified "2023-09-26" @default.
- W2809860508 title "Intensification of Aqueous Two-phase Extraction for Protein Purification" @default.
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- W2809860508 doi "https://doi.org/10.1039/9781788010320-00344" @default.
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