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• W2809961663 abstract "Background Perturbations of epidermal and follicular homeostasis have been attributed to a variety of skin diseases affecting dogs. The availability of an in vitro system to investigate these diseases is important to understand underlying pathomechanisms. Objectives To establish an accurate and reliable in vitro 3D system of canine keratinocyte organoids to lay the basis for studying functional defects in interfollicular epidermis ( IFE ) and hair follicle ( HF ) morphogenesis, reconstitution and differentiation that lead to alopecic and epidermal diseases. Animals Skin biopsies were obtained from freshly euthanized dogs of different breeds with no skin abnormalities. Methods Cells derived from microdissected IFE and HF s were seeded in Matrigel and keratinocyte organoids were grown and characterized using immunohistochemistry, RT ‐ qPCR and RNA sequencing. Results Both organoid lines develop into a basal IFE ‐like cell type. Gene and protein expression analysis revealed high mRNA and protein levels of keratins 5 and 14, IFE differentiation markers and intercellular molecules. Key markers of HF stem cells were lacking. Withdrawal of growth factors resulted in upregulation of markers such as KRT 16, Involucrin, KRT 17 and SOX 9, showing the potential of the organoids to develop towards more differentiated tissue. Conclusion and clinical importance Our 3D in vitro culture system provides the basis to explore epidermal function, to investigate the culture conditions necessary for the development of organoids with a HF signature and to address cutaneous disorders in dogs. However, for induction of HF signatures or hair growth, addition of different growth factors or co‐culture with dermal papilla will be required." @default.
• W2809961663 created "2018-07-10" @default.
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• W2809961663 date "2018-07-02" @default.
• W2809961663 modified "2023-10-01" @default.
• W2809961663 title "Establishment and characterization of a canine keratinocyte organoid culture system" @default.
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• W2809961663 doi "https://doi.org/10.1111/vde.12541" @default.
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