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- W2810167907 abstract "Introduction: Magnetic nanoparticles (MNPs) are one of the most useful particulate systems in analytical applications such as specific aptamer selection. Proteins are the most noted targets of aptamer selection. Generally, covalently immobilized protein coated MNPs are more stable structures. Methods: In this study, coagulation factor VIII (FVIII) was immobilized on MNPs. A silica coating provided isocyanate functional groups was considered to interact covalently with reactive groups of the protein, resulting in a stable protein immobilization. The reactions was run in dried toluene. At end, these MNPs were applied for affinity determination of a previously selected FVIII specific aptamers. Results: Immobilization of 1 mg FVIII (~ 3 nmol) on 5 mg particles was achieved with no significant particle aggregation. Using a fluorescence-based method, affinity measurement resulted in a calculated dissociation constant of 120 ± 5.6 nM for the FVIII-specific aptamer to the FVIII-coated MNPs. Conclusion: The final product could be a suitable protein-coated solid support for magnetic-based aptamer selection processes." @default.
- W2810167907 created "2018-07-10" @default.
- W2810167907 creator A5000699715 @default.
- W2810167907 creator A5003572154 @default.
- W2810167907 creator A5045780428 @default.
- W2810167907 date "2018-04-02" @default.
- W2810167907 modified "2023-09-25" @default.
- W2810167907 title "Covalent immobilization of coagulation factor VIII on magnetic nanoparticles for aptamer development" @default.
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- W2810167907 doi "https://doi.org/10.1177/2280800018765046" @default.
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