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- W281027450 abstract "The biology of the human filaria Mansonella perstans has been poorly studied due in part to the lack of experimental animal models in which its life-cycle could be reproduced. In nature Culicodes spp. (and probably simuliids) are the vectors involved in disease transmission. In the present work Aedes aegypti black-eyed strain was experimentally infected with M. perstans microfilariae by intrathoracic inoculation of 8 to 15 parasites contained in 0.4-0.6 microliters of RPMI 1640. Concentration of microfilariae was achieved by means of a ficoll separation technique. A. aegypti were maintained at 26 degrees C and 80% relative humidity with a sugar-water diet, except on day 4 post-infection on which they received an uninfected blood meal. Larval development was slow, taking no less than 4 days to reach the sausage stage, which measured 95-100 microns in length. Molt to the second stage took place on the 6th day; the differentiation into a long muscular and glandular esophagus, and short intestine measuring approximately one fourth of the total larval length were the most significant changes. At the end of this period 4 small but well demarcated tail papillae were observed. The first third-stage larvae appeared at the 8th day post-infection, measuring 650 to 680 microns in length. Beyond the 10th day larvae with an average length of 750 microns were found in the thorax, head, and mouthparts. Four conspicuous tail papillae characteristic of the genus Mansonella were seen in all third-stage larvae.(ABSTRACT TRUNCATED AT 250 WORDS)" @default.
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- W281027450 date "1990-12-01" @default.
- W281027450 modified "2023-09-23" @default.
- W281027450 title "Development of Mansonella perstans in the surrogate vector Aedes aegypti." @default.
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