FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2810285918> ?p ?o ?g. }

• W2810285918 endingPage "472" @default.
• W2810285918 startingPage "461" @default.
• W2810285918 abstract "G-quadruplexes (G4) are nucleic acid secondary structures frequently assumed by G-rich sequences located mostly at telomeres and proto-oncogenes promoters. Recently, we identified, in canine <i>KIT</i> (v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog) promoter, two G-rich sequences able to fold into G4: d_kit1 and d_kit2_A16. In this study, an anthraquinone (AQ1) and an anthracene derivative (AN6), known to stabilize the G4 structures of the corresponding human h_kit1 and h_kit2, were tested on the canine G4 and in two canine mast cell tumor (MCT) cell lines (C2 and NI-1) to verify their capability to down-regulate <i>KIT</i> expression. The cytotoxicity of AQ1 and AN6 was determined using the Alamar Blue test; also the constitutive expression of <i>KIT</i> and other proto-oncogenes containing G4 structures in their promoter (<i>BCL2</i>, <i>VEGFα</i>, <i>VEGFR2</i>, <i>KRAS</i>, and <i>TERT</i>) was assessed by quantitative real-time polymerase chain reaction (qRT-PCR). Then the time- and dose-dependent effects of both ligands on target gene expression were assessed by qRT-PCR. All target genes were constitutively expressed up to 96 hours of culture. Both ligands decreased <i>KIT</i> mRNA levels and c-kit protein amount, and AN6 was comparatively fairly more effective. DNA interaction studies and a dual-luciferase gene reporter assay performed on a noncancerous canine cell line (Madin-Darby Canine Kidney cells) proved that this down-regulation was the result of the interaction of AN6 with <i>KIT</i> proximal promoter. Interestingly, our results only partially overlap with those previously obtained in human cell lines, where AQ1 was found as the most effective compound. These preliminary data might suggest AN6 as a promising candidate for the selective targeting of canine <i>KIT</i>-dependent tumors." @default.
• W2810285918 created "2018-07-10" @default.
• W2810285918 creator A5001638640 @default.
• W2810285918 creator A5052906679 @default.
• W2810285918 creator A5053470485 @default.
• W2810285918 creator A5055297886 @default.
• W2810285918 creator A5061831978 @default.
• W2810285918 creator A5066082205 @default.
• W2810285918 creator A5066139935 @default.
• W2810285918 creator A5075876145 @default.
• W2810285918 date "2018-10-01" @default.
• W2810285918 modified "2023-10-12" @default.
• W2810285918 title "Targeting Canine <i>KIT</i> Promoter by Candidate DNA G-Quadruplex Ligands" @default.
• W2810285918 cites W1894349587 @default.
• W2810285918 cites W1914649288 @default.
• W2810285918 cites W1970574292 @default.
• W2810285918 cites W1974663878 @default.
• W2810285918 cites W1983042146 @default.
• W2810285918 cites W1990635146 @default.
• W2810285918 cites W1991797965 @default.
• W2810285918 cites W1995500704 @default.
• W2810285918 cites W1998838965 @default.
• W2810285918 cites W2007634776 @default.
• W2810285918 cites W2008601164 @default.
• W2810285918 cites W2016968345 @default.
• W2810285918 cites W2026370504 @default.
• W2810285918 cites W2027696068 @default.
• W2810285918 cites W2043729017 @default.
• W2810285918 cites W2044245959 @default.
• W2810285918 cites W2045169349 @default.
• W2810285918 cites W2050913592 @default.
• W2810285918 cites W2052429686 @default.
• W2810285918 cites W2059306569 @default.
• W2810285918 cites W2063733156 @default.
• W2810285918 cites W2065939410 @default.
• W2810285918 cites W2071442155 @default.
• W2810285918 cites W2079409234 @default.
• W2810285918 cites W2080563014 @default.
• W2810285918 cites W2085854162 @default.
• W2810285918 cites W2087520481 @default.
• W2810285918 cites W2106986615 @default.
• W2810285918 cites W2107277218 @default.
• W2810285918 cites W2119449687 @default.
• W2810285918 cites W2124572106 @default.
• W2810285918 cites W2124582915 @default.
• W2810285918 cites W2129522390 @default.
• W2810285918 cites W2148660588 @default.
• W2810285918 cites W2149908540 @default.
• W2810285918 cites W2150951551 @default.
• W2810285918 cites W2153879694 @default.
• W2810285918 cites W2163134391 @default.
• W2810285918 cites W2170552391 @default.
• W2810285918 cites W2289936101 @default.
• W2810285918 cites W2312596574 @default.
• W2810285918 cites W2326168525 @default.
• W2810285918 cites W2409531891 @default.
• W2810285918 cites W2626836216 @default.
• W2810285918 cites W2762665016 @default.
• W2810285918 cites W350315305 @default.
• W2810285918 cites W4240013025 @default.
• W2810285918 cites W943910173 @default.
• W2810285918 doi "https://doi.org/10.1124/jpet.118.248997" @default.
• W2810285918 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30275152" @default.
• W2810285918 hasPublicationYear "2018" @default.
• W2810285918 type Work @default.
• W2810285918 sameAs 2810285918 @default.
• W2810285918 citedByCount "5" @default.
• W2810285918 countsByYear W28102859182018 @default.
• W2810285918 countsByYear W28102859182019 @default.
• W2810285918 countsByYear W28102859182020 @default.
• W2810285918 countsByYear W28102859182021 @default.
• W2810285918 crossrefType "journal-article" @default.
• W2810285918 hasAuthorship W2810285918A5001638640 @default.
• W2810285918 hasAuthorship W2810285918A5052906679 @default.
• W2810285918 hasAuthorship W2810285918A5053470485 @default.
• W2810285918 hasAuthorship W2810285918A5055297886 @default.
• W2810285918 hasAuthorship W2810285918A5061831978 @default.
• W2810285918 hasAuthorship W2810285918A5066082205 @default.
• W2810285918 hasAuthorship W2810285918A5066139935 @default.
• W2810285918 hasAuthorship W2810285918A5075876145 @default.
• W2810285918 hasBestOaLocation W28102859181 @default.
• W2810285918 hasConcept C101762097 @default.
• W2810285918 hasConcept C104317684 @default.
• W2810285918 hasConcept C150194340 @default.
• W2810285918 hasConcept C153911025 @default.
• W2810285918 hasConcept C161733203 @default.
• W2810285918 hasConcept C185592680 @default.
• W2810285918 hasConcept C24107716 @default.
• W2810285918 hasConcept C502942594 @default.
• W2810285918 hasConcept C54355233 @default.
• W2810285918 hasConcept C552990157 @default.
• W2810285918 hasConcept C55493867 @default.
• W2810285918 hasConcept C81885089 @default.
• W2810285918 hasConcept C83762044 @default.
• W2810285918 hasConcept C86803240 @default.
• W2810285918 hasConceptScore W2810285918C101762097 @default.
• W2810285918 hasConceptScore W2810285918C104317684 @default.
• W2810285918 hasConceptScore W2810285918C150194340 @default.

• next