FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2810306332> ?p ?o ?g. }

• W2810306332 endingPage "1038" @default.
• W2810306332 startingPage "1031" @default.
• W2810306332 abstract "Background: Effective digestive enzymes are crucial for successful islet isolation. Supplemental proteases are essential because they synergize with collagenase for effective pancreatic digestion. The activity of these enzymes is critically dependent on the presence of Ca 2+ ions at a concentration of 5–10 mM. The present study aimed to determine the Ca 2+ concentration during human islet isolation and to ascertain whether the addition of supplementary Ca 2+ is required to maintain an optimal Ca 2+ concentration during the various phases of the islet isolation process. Methods: Human islets were isolated according to standard methods and isolation parameters. Islet quality control and the number of isolations fulfilling standard transplantation criteria were evaluated. Ca 2+ was determined by using standard clinical chemistry routines. Islet isolation was performed with or without addition of supplementary Ca 2+ to reach a Ca 2+ of 5 mM. Results: Ca 2+ concentration was markedly reduced in bicarbonate-based buffers, especially if additional bicarbonate was used to adjust the pH as recommended by the Clinical Islet Transplantation Consortium. A major reduction in Ca 2+ concentration was also observed during pancreatic enzyme perfusion, digestion, and harvest. Additional Ca 2+ supplementation of media used for dissolving the enzymes and during digestion, perfusion, and harvest was necessary in order to obtain the concentration recommended for optimal enzyme activity and efficient liberation of a large number of islets from the human pancreas. Conclusions: Ca 2+ is to a large extent consumed during clinical islet isolation, and in the absence of supplementation, the concentration fell below that recommended for optimal enzyme activity. Ca 2+ supplementation of the media used during human pancreas digestion is necessary to maintain the concentration recommended for optimal enzyme activity. Addition of Ca 2+ to the enzyme blend has been implemented in the standard isolation protocols in the Nordic Network for Clinical Islet Transplantation." @default.
• W2810306332 created "2018-07-10" @default.
• W2810306332 creator A5003029542 @default.
• W2810306332 creator A5017913712 @default.
• W2810306332 creator A5020673711 @default.
• W2810306332 creator A5023864554 @default.
• W2810306332 creator A5024948038 @default.
• W2810306332 creator A5047686333 @default.
• W2810306332 creator A5052216601 @default.
• W2810306332 creator A5055291545 @default.
• W2810306332 creator A5055673489 @default.
• W2810306332 creator A5066063403 @default.
• W2810306332 creator A5086842971 @default.
• W2810306332 creator A5091837697 @default.
• W2810306332 date "2018-06-26" @default.
• W2810306332 modified "2023-10-06" @default.
• W2810306332 title "Calcium" @default.
• W2810306332 cites W1189869135 @default.
• W2810306332 cites W1497181681 @default.
• W2810306332 cites W1595914069 @default.
• W2810306332 cites W2002483584 @default.
• W2810306332 cites W2027054547 @default.
• W2810306332 cites W2035582064 @default.
• W2810306332 cites W2040919076 @default.
• W2810306332 cites W2066795948 @default.
• W2810306332 cites W2073282815 @default.
• W2810306332 cites W2081502711 @default.
• W2810306332 cites W2081972846 @default.
• W2810306332 cites W2092702917 @default.
• W2810306332 cites W2318943386 @default.
• W2810306332 cites W2341414956 @default.
• W2810306332 cites W2539044895 @default.
• W2810306332 cites W2549765016 @default.
• W2810306332 cites W2700444418 @default.
• W2810306332 doi "https://doi.org/10.1177/0963689718779350" @default.
• W2810306332 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6158545" @default.
• W2810306332 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29945463" @default.
• W2810306332 hasPublicationYear "2018" @default.
• W2810306332 type Work @default.
• W2810306332 sameAs 2810306332 @default.
• W2810306332 citedByCount "7" @default.
• W2810306332 countsByYear W28103063322020 @default.
• W2810306332 countsByYear W28103063322021 @default.
• W2810306332 countsByYear W28103063322022 @default.
• W2810306332 countsByYear W28103063322023 @default.
• W2810306332 crossrefType "journal-article" @default.
• W2810306332 hasAuthorship W2810306332A5003029542 @default.
• W2810306332 hasAuthorship W2810306332A5017913712 @default.
• W2810306332 hasAuthorship W2810306332A5020673711 @default.
• W2810306332 hasAuthorship W2810306332A5023864554 @default.
• W2810306332 hasAuthorship W2810306332A5024948038 @default.
• W2810306332 hasAuthorship W2810306332A5047686333 @default.
• W2810306332 hasAuthorship W2810306332A5052216601 @default.
• W2810306332 hasAuthorship W2810306332A5055291545 @default.
• W2810306332 hasAuthorship W2810306332A5055673489 @default.
• W2810306332 hasAuthorship W2810306332A5066063403 @default.
• W2810306332 hasAuthorship W2810306332A5086842971 @default.
• W2810306332 hasAuthorship W2810306332A5091837697 @default.
• W2810306332 hasBestOaLocation W28103063321 @default.
• W2810306332 hasConcept C126322002 @default.
• W2810306332 hasConcept C134018914 @default.
• W2810306332 hasConcept C146957229 @default.
• W2810306332 hasConcept C165220095 @default.
• W2810306332 hasConcept C16685009 @default.
• W2810306332 hasConcept C181199279 @default.
• W2810306332 hasConcept C182220744 @default.
• W2810306332 hasConcept C185592680 @default.
• W2810306332 hasConcept C2778394429 @default.
• W2810306332 hasConcept C2778764654 @default.
• W2810306332 hasConcept C2779306644 @default.
• W2810306332 hasConcept C2781112554 @default.
• W2810306332 hasConcept C2781357212 @default.
• W2810306332 hasConcept C2911091166 @default.
• W2810306332 hasConcept C43617362 @default.
• W2810306332 hasConcept C519063684 @default.
• W2810306332 hasConcept C55493867 @default.
• W2810306332 hasConcept C71924100 @default.
• W2810306332 hasConceptScore W2810306332C126322002 @default.
• W2810306332 hasConceptScore W2810306332C134018914 @default.
• W2810306332 hasConceptScore W2810306332C146957229 @default.
• W2810306332 hasConceptScore W2810306332C165220095 @default.
• W2810306332 hasConceptScore W2810306332C16685009 @default.
• W2810306332 hasConceptScore W2810306332C181199279 @default.
• W2810306332 hasConceptScore W2810306332C182220744 @default.
• W2810306332 hasConceptScore W2810306332C185592680 @default.
• W2810306332 hasConceptScore W2810306332C2778394429 @default.
• W2810306332 hasConceptScore W2810306332C2778764654 @default.
• W2810306332 hasConceptScore W2810306332C2779306644 @default.
• W2810306332 hasConceptScore W2810306332C2781112554 @default.
• W2810306332 hasConceptScore W2810306332C2781357212 @default.
• W2810306332 hasConceptScore W2810306332C2911091166 @default.
• W2810306332 hasConceptScore W2810306332C43617362 @default.
• W2810306332 hasConceptScore W2810306332C519063684 @default.
• W2810306332 hasConceptScore W2810306332C55493867 @default.
• W2810306332 hasConceptScore W2810306332C71924100 @default.
• W2810306332 hasFunder F4320323044 @default.
• W2810306332 hasIssue "7" @default.
• W2810306332 hasLocation W28103063321 @default.

• next