Matches in SemOpenAlex for { <https://semopenalex.org/work/W2810347467> ?p ?o ?g. }
- W2810347467 endingPage "844" @default.
- W2810347467 startingPage "837" @default.
- W2810347467 abstract "Conventional direct microscopy with potassium hydroxide (KOH) and culture were found to lack the ability to establish a fast and specific diagnosis of dermatophytosis. A pan-dermatophyte nested-PCR assay was developed using a novel primer pair targeting the translation elongation factor 1-α (Tef-1α) sequences for direct detection and identification of most veterinary relevant dermatophytes in animal samples suspected to dermatophytosis. A total of 140 animal skin and hair samples were subjected to direct microscopy, culture, and ITS-RFLP/ITS-sequencing of culture isolates for the detection and identification of dermatophytosis agents. Nested-PCR sequencing was performed on all the extracted DNAs using a commercial kit after dissolving the specimens by mechanical beating. Nested-PCR was positive in 90% of samples, followed by direct microscopy (85.7%) and culture (75%). The degree of agreement between nested-PCR and direct microscopy (94.4%) was higher than with culture (83.3%). In 105 culture-positive cases, the measures of agreement for the identification of dermatophytosis agents were as follows: 100% between nested-PCR sequencing and ITS-RFLP/ITS-sequencing and 63.8% between nested-PCR sequencing and culture. The developed nested-PCR was faster as well as more sensitive and specific than conventional methods for detection and identification of dermatophytes in clinical samples, which was particularly suitable for epidemiological studies." @default.
- W2810347467 created "2018-07-10" @default.
- W2810347467 creator A5010843536 @default.
- W2810347467 creator A5027458237 @default.
- W2810347467 creator A5029665417 @default.
- W2810347467 creator A5046225875 @default.
- W2810347467 creator A5087860103 @default.
- W2810347467 creator A5091614725 @default.
- W2810347467 date "2018-07-11" @default.
- W2810347467 modified "2023-09-25" @default.
- W2810347467 title "Assessment of a pan-dermatophyte nested-PCR compared with conventional methods for direct detection and identification of dermatophytosis agents in animals" @default.
- W2810347467 cites W1557918476 @default.
- W2810347467 cites W1563751765 @default.
- W2810347467 cites W1747217985 @default.
- W2810347467 cites W1768734704 @default.
- W2810347467 cites W1985533724 @default.
- W2810347467 cites W2005731819 @default.
- W2810347467 cites W2033566398 @default.
- W2810347467 cites W2035204961 @default.
- W2810347467 cites W2056901298 @default.
- W2810347467 cites W2070454248 @default.
- W2810347467 cites W2099285518 @default.
- W2810347467 cites W2099992514 @default.
- W2810347467 cites W2109108201 @default.
- W2810347467 cites W2112231022 @default.
- W2810347467 cites W2118097144 @default.
- W2810347467 cites W2119979025 @default.
- W2810347467 cites W2120644786 @default.
- W2810347467 cites W2148500953 @default.
- W2810347467 cites W2163378561 @default.
- W2810347467 cites W2206082779 @default.
- W2810347467 cites W2277570497 @default.
- W2810347467 cites W2410638621 @default.
- W2810347467 cites W2413817970 @default.
- W2810347467 cites W2533504273 @default.
- W2810347467 cites W2735460790 @default.
- W2810347467 cites W2789905074 @default.
- W2810347467 cites W4211087045 @default.
- W2810347467 doi "https://doi.org/10.1111/myc.12821" @default.
- W2810347467 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29944743" @default.
- W2810347467 hasPublicationYear "2018" @default.
- W2810347467 type Work @default.
- W2810347467 sameAs 2810347467 @default.
- W2810347467 citedByCount "13" @default.
- W2810347467 countsByYear W28103474672018 @default.
- W2810347467 countsByYear W28103474672019 @default.
- W2810347467 countsByYear W28103474672020 @default.
- W2810347467 countsByYear W28103474672021 @default.
- W2810347467 countsByYear W28103474672022 @default.
- W2810347467 countsByYear W28103474672023 @default.
- W2810347467 crossrefType "journal-article" @default.
- W2810347467 hasAuthorship W2810347467A5010843536 @default.
- W2810347467 hasAuthorship W2810347467A5027458237 @default.
- W2810347467 hasAuthorship W2810347467A5029665417 @default.
- W2810347467 hasAuthorship W2810347467A5046225875 @default.
- W2810347467 hasAuthorship W2810347467A5087860103 @default.
- W2810347467 hasAuthorship W2810347467A5091614725 @default.
- W2810347467 hasBestOaLocation W28103474672 @default.
- W2810347467 hasConcept C104317684 @default.
- W2810347467 hasConcept C157431215 @default.
- W2810347467 hasConcept C17744445 @default.
- W2810347467 hasConcept C178790620 @default.
- W2810347467 hasConcept C182957341 @default.
- W2810347467 hasConcept C185592680 @default.
- W2810347467 hasConcept C199539241 @default.
- W2810347467 hasConcept C2777220567 @default.
- W2810347467 hasConcept C2777563447 @default.
- W2810347467 hasConcept C49105822 @default.
- W2810347467 hasConcept C54355233 @default.
- W2810347467 hasConcept C86803240 @default.
- W2810347467 hasConcept C89423630 @default.
- W2810347467 hasConceptScore W2810347467C104317684 @default.
- W2810347467 hasConceptScore W2810347467C157431215 @default.
- W2810347467 hasConceptScore W2810347467C17744445 @default.
- W2810347467 hasConceptScore W2810347467C178790620 @default.
- W2810347467 hasConceptScore W2810347467C182957341 @default.
- W2810347467 hasConceptScore W2810347467C185592680 @default.
- W2810347467 hasConceptScore W2810347467C199539241 @default.
- W2810347467 hasConceptScore W2810347467C2777220567 @default.
- W2810347467 hasConceptScore W2810347467C2777563447 @default.
- W2810347467 hasConceptScore W2810347467C49105822 @default.
- W2810347467 hasConceptScore W2810347467C54355233 @default.
- W2810347467 hasConceptScore W2810347467C86803240 @default.
- W2810347467 hasConceptScore W2810347467C89423630 @default.
- W2810347467 hasFunder F4320311405 @default.
- W2810347467 hasFunder F4320323048 @default.
- W2810347467 hasIssue "11" @default.
- W2810347467 hasLocation W28103474671 @default.
- W2810347467 hasLocation W28103474672 @default.
- W2810347467 hasLocation W28103474673 @default.
- W2810347467 hasOpenAccess W2810347467 @default.
- W2810347467 hasPrimaryLocation W28103474671 @default.
- W2810347467 hasRelatedWork W1964769696 @default.
- W2810347467 hasRelatedWork W1970238058 @default.
- W2810347467 hasRelatedWork W1992501950 @default.
- W2810347467 hasRelatedWork W2019810109 @default.
- W2810347467 hasRelatedWork W2116837959 @default.
- W2810347467 hasRelatedWork W2164216328 @default.