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• W2810360150 endingPage "4288" @default.
• W2810360150 startingPage "4276" @default.
• W2810360150 abstract "Naturally occurring mutations found in one of the two Ω-loop substructures in human cytochrome c are associated with low blood platelet count (thrombocytopenia). Both Ω-loops participate in the formation of conformers associated with cytochrome c peroxidase activity and apoptotic function. At alkaline pH values, the Met80 ligand to the ferric heme iron dissociates, and a lysine residue in the 71-85 Ω-loop coordinates to the iron. The alkaline isomerization has been the focus of extensive kinetic studies, and it is established that a deprotonation triggers the release of the Met80 ligand (p Ktrigger). A second deprotonation stabilizes a pentacoordinate heme form (p Ka2). In this study, site-directed variants at the 41 and 48 positions in the 40-57 Ω-loop and at the 81 and 83 positions in the 71-85 Ω-loop reveal that conformational transitions in the 71-85 Ω-loop, leading to the alkaline or peroxidatic conformers, are controlled by the 40-57 Ω-loop. We find that the variants causing thrombocytopenia, G41S and Y48H, lower the p Ktrigger and increase p Ka2. Our results are presented in a mechanistic framework, depicted by a cube, that accounts for the pH dependencies of the equilibrium and kinetic parameters governing the alkaline transition of the native protein and Ω-loop variants. The data are most consistent with the trigger for Met80 replacement by a lysine being a deprotonation within a hydrogen bonded unit that links the two Ω-loops rather than an individual group. Such a proposal aligns with the entatic contribution made by the same unit in controlling the Met80-Fe(III) bond strength." @default.
• W2810360150 created "2018-07-10" @default.
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• W2810360150 date "2018-06-27" @default.
• W2810360150 modified "2023-10-12" @default.
• W2810360150 title "Naturally Occurring Disease-Related Mutations in the 40–57 Ω-Loop of Human Cytochrome <i>c</i> Control Triggering of the Alkaline Isomerization" @default.
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• W2810360150 cites W1719730487 @default.
• W2810360150 cites W1959985516 @default.
• W2810360150 cites W1963907102 @default.
• W2810360150 cites W1968363241 @default.
• W2810360150 cites W1973664438 @default.
• W2810360150 cites W1982658197 @default.
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• W2810360150 doi "https://doi.org/10.1021/acs.biochem.8b00520" @default.
• W2810360150 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29949346" @default.
• W2810360150 hasPublicationYear "2018" @default.
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