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• W2810565547 abstract "Glycerol is one of the most important by-products of alcohol fermentation, and depending on its concentration it can contribute to wine flavor intensity and aroma volatility. Here, we evaluated the potential of utilizing the natural genetic variation of non-coding regions in budding yeast to identify allelic variants that could modulate glycerol phenotype during wine fermentation. For this we utilized four Saccharomyces cerevisiae strains (WE - Wine/European, SA - Sake, NA - North American, and WA - West African), which were previously profiled for genome-wide Allele Specific Expression (ASE) levels. The glycerol yields under Synthetic Wine Must (SWM) fermentations differed significantly between strains; WA produced the highest glycerol yields while SA produced the lowest yields. Subsequently, from our ASE database, we identified two candidate genes involved in alcoholic fermentation pathways, ADH3 and GPD1, exhibiting significant expression differences between strains. A reciprocal hemizygosity assay demonstrated that hemizygotes expressing GPD1WA , GPD1SA , ADH3WA and ADH3SA alleles had significantly greater glycerol yields compared to GPD1WE and ADH3WE . We further analyzed the gene expression profiles for each GPD1 variant under SWM, demonstrating that the expression of GPD1WE occurred earlier and was greater compared to the other alleles. This result indicates that the level, timing, and condition of expression differ between regulatory regions in the various genetic backgrounds. Furthermore, promoter allele swapping demonstrated that these allele expression patterns were transposable across genetic backgrounds; however, glycerol yields did not differ between wild type and modified strains, suggesting a strong trans effect on GPD1 gene expression. In this line, Gpd1 protein levels in parental strains, particularly Gpd1pWE, did not necessarily correlate with gene expression differences, but rather with glycerol yield where low Gpd1pWE levels were detected. This suggests that GPD1WE is influenced by recessive negative post-transcriptional regulation which is absent in the other genetic backgrounds. This dissection of regulatory mechanisms in GPD1 allelic variants demonstrates the potential to exploit natural alleles to improve glycerol production in wine fermentation and highlights the difficulties of trait improvement due to alternative trans-regulation and gene-gene interactions in the different genetic background." @default.
• W2810565547 created "2018-07-10" @default.
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• W2810565547 date "2018-07-03" @default.
• W2810565547 modified "2023-10-16" @default.
• W2810565547 title "GPD1 and ADH3 Natural Variants Underlie Glycerol Yield Differences in Wine Fermentation" @default.
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• W2810565547 cites W1848905263 @default.
• W2810565547 cites W1915104237 @default.
• W2810565547 cites W1964410962 @default.
• W2810565547 cites W1965408541 @default.
• W2810565547 cites W1977547738 @default.
• W2810565547 cites W1977848450 @default.
• W2810565547 cites W1995340594 @default.
• W2810565547 cites W2001228080 @default.
• W2810565547 cites W2004622897 @default.
• W2810565547 cites W2005019160 @default.
• W2810565547 cites W2007193968 @default.
• W2810565547 cites W2011652579 @default.
• W2810565547 cites W2012354488 @default.
• W2810565547 cites W2017208073 @default.
• W2810565547 cites W2017784165 @default.
• W2810565547 cites W2027980770 @default.
• W2810565547 cites W2030820568 @default.
• W2810565547 cites W2039279598 @default.
• W2810565547 cites W2039288158 @default.
• W2810565547 cites W2043474387 @default.
• W2810565547 cites W2055345776 @default.
• W2810565547 cites W2063347034 @default.
• W2810565547 cites W2074368204 @default.
• W2810565547 cites W2075396966 @default.
• W2810565547 cites W2087948270 @default.
• W2810565547 cites W2093842279 @default.
• W2810565547 cites W2094248448 @default.
• W2810565547 cites W2095295625 @default.
• W2810565547 cites W2097715142 @default.
• W2810565547 cites W2098249598 @default.
• W2810565547 cites W2103869992 @default.
• W2810565547 cites W2104262114 @default.
• W2810565547 cites W2106245349 @default.
• W2810565547 cites W2110037042 @default.
• W2810565547 cites W2110876945 @default.
• W2810565547 cites W2116595900 @default.
• W2810565547 cites W2117247186 @default.
• W2810565547 cites W2121331980 @default.
• W2810565547 cites W2121547736 @default.
• W2810565547 cites W2127072693 @default.
• W2810565547 cites W2127298691 @default.
• W2810565547 cites W2132488713 @default.
• W2810565547 cites W2132815796 @default.
• W2810565547 cites W2139522960 @default.
• W2810565547 cites W2140412033 @default.
• W2810565547 cites W2140870757 @default.
• W2810565547 cites W2146506602 @default.
• W2810565547 cites W2157330233 @default.
• W2810565547 cites W2157613837 @default.
• W2810565547 cites W2159100436 @default.
• W2810565547 cites W2163819860 @default.
• W2810565547 cites W2165762336 @default.
• W2810565547 cites W2167120442 @default.
• W2810565547 cites W2176343348 @default.
• W2810565547 cites W2301143347 @default.
• W2810565547 cites W2336675821 @default.
• W2810565547 cites W2340761255 @default.
• W2810565547 cites W2434478718 @default.
• W2810565547 cites W2528598248 @default.
• W2810565547 cites W2587992888 @default.
• W2810565547 cites W2624169519 @default.
• W2810565547 cites W2793210640 @default.
• W2810565547 cites W2798064943 @default.
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• W2810565547 doi "https://doi.org/10.3389/fmicb.2018.01460" @default.
• W2810565547 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6037841" @default.
• W2810565547 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30018610" @default.
• W2810565547 hasPublicationYear "2018" @default.
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