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- W2810697327 abstract "Tomato (Solanum lycopersicum L.) is the second most producedand consumed vegetable, next to potato. Pusa early dwarf (PED)variety of tomato is sensitive to salinity and drought which reducesthe yield and fruit quality in tomato. In the present study,tomato transgenics were developed with osmotin-like protein(OLP) and chitinase (Chi11) genes via in vitro and in plantatransformation methods. Transgene integration and transcriptlevels were confirmed by multiplex PCR, DNA blot, and multiplexreverse transcriptase PCR. Homozygous T2 transgenics,when evaluated for salt and drought stress, showed enhancedtolerance compared to wild-type plants. Transgenics showedenhanced root biomass under normal conditions. Transformantsalso displayed higher proline content, K+, relative water content,chlorophyll fluorescence, total biomass, vascular conductivity,and fruit yield compared to the wild-type plants under stressconditions. Co-immunoprecipitation revealed that Chi11 co-expresseswith phosphofructokinase2 (PFK2), which may play arole in enhanced root biomass. qPCR analysis resulted in highertranscript levels of OLP, Chi11, and PFK2 in transgenics as comparedto the wild-type plants." @default.
- W2810697327 created "2018-07-10" @default.
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- W2810697327 date "2017-02-01" @default.
- W2810697327 modified "2023-09-24" @default.
- W2810697327 title "Development of transgenic tomato (Solanum lycoperscicum L.) by heterologous expression of osmotin-like protein (OLP) and chitinase (Chi11) genes for salt and drought stress tolerance" @default.
- W2810697327 hasPublicationYear "2017" @default.
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